Alessandra Orfano
Associate Research Scientist in Epidemiology (Microbial Diseases); Affiliated Faculty, Yale Institute for Global Health
Research & Publications
Biography
Coauthors
Selected Publications
- Repeatability and reproducibility of a handheld quantitative G6PD diagnosticLey B, Satyagraha A, Kibria MG, Armstrong J, Bancone G, Bei AK, Bizilj G, Brito M, Ding XC, Domingo GJ, von Fricken ME, Gornsawun G, Lam B, Menard D, Monteiro W, Ongarello S, Pal S, Panggalo LV, Parikh S, Pfeffer DA, Price RN, da Silva Orfano A, Wade M, Wojnarski M, Worachet K, Yar A, Alam MS, Howes RE. Repeatability and reproducibility of a handheld quantitative G6PD diagnostic. PLOS Neglected Tropical Diseases 2022, 16: e0010174. PMID: 35176015, PMCID: PMC8853557, DOI: 10.1371/journal.pntd.0010174.
- Phenotypic traits of individuals in a long-term colony of Anopheles (Nyssorhynchus) aquasalis (Diptera: Culicidae) show variable susceptibility to Plasmodium and suggest cryptic speciation.Rodrigues NB, Villegas LEM, Duarte APM, Orfanó AS, Dos Anjos Costa B, Nacif Pimenta R, Lacerda MVG, Monteiro WM, Secundino NFC, Pimenta PFP. Phenotypic traits of individuals in a long-term colony of Anopheles (Nyssorhynchus) aquasalis (Diptera: Culicidae) show variable susceptibility to Plasmodium and suggest cryptic speciation. Acta Tropica 2021, 224: 106129. PMID: 34509458, DOI: 10.1016/j.actatropica.2021.106129.
- Dengue and Zika virus infection patterns vary among Aedes aegypti field populations from Belo Horizonte, a Brazilian endemic cityGodoy RSM, dos Santos Felix L, da Silva Orfanó A, Chaves BA, Nogueira PM, dos Anjos Costa B, Soares AS, Oliveira CCA, Nacif-Pimenta R, Silva BM, Duarte AP, de Lacerda M, Monteiro WM, Secundino NFC, Pimenta PFP. Dengue and Zika virus infection patterns vary among Aedes aegypti field populations from Belo Horizonte, a Brazilian endemic city. PLOS Neglected Tropical Diseases 2021, 15: e0009839. PMID: 34727099, PMCID: PMC8562804, DOI: 10.1371/journal.pntd.0009839.
- Brazilian Aedes aegypti as a Competent Vector for Multiple Complex Arboviral Coinfections.Rodrigues NB, Godoy RSM, Orfano AS, Chaves BA, Campolina TB, Costa BDA, Félix LDS, Silva BM, Norris DE, Pimenta PFP, Secundino NFC. Brazilian Aedes aegypti as a Competent Vector for Multiple Complex Arboviral Coinfections. The Journal Of Infectious Diseases 2021, 224: 101-108. PMID: 33544850, DOI: 10.1093/infdis/jiab066.
- Paratransgenic manipulation of a tsetse microRNA alters the physiological homeostasis of the fly’s midgut environmentYang L, Weiss BL, Williams AE, Aksoy E, de Silva Orfano A, Son JH, Wu Y, Vigneron A, Karakus M, Aksoy S. Paratransgenic manipulation of a tsetse microRNA alters the physiological homeostasis of the fly’s midgut environment. PLOS Pathogens 2021, 17: e1009475. PMID: 34107000, PMCID: PMC8216540, DOI: 10.1371/journal.ppat.1009475.
- Ultrastructure of the Antennae and Sensilla of Nyssomyia intermedia (Diptera: Psychodidae), Vector of American Cutaneous Leishmaniasis.Fernandes FF, Barletta ABF, Orfanó AS, Pinto LC, Nacif-Pimenta R, Miranda JC, Secundino NFC, Bahia AC, Pimenta PFP. Ultrastructure of the Antennae and Sensilla of Nyssomyia intermedia (Diptera: Psychodidae), Vector of American Cutaneous Leishmaniasis. Journal Of Medical Entomology 2020, 57: 1722-1734. PMID: 32761144, PMCID: PMC7899269, DOI: 10.1093/jme/tjaa124.
- Microanatomy of the American Malaria Vector Anopheles aquasalis (Diptera: Culicidae: Anophelinae) Midgut: Ultrastructural and Histochemical Observations.Baia-da-Silva DC, Orfanó AS, Nacif-Pimenta R, de Melo FF, Guerra MGVB, Lacerda MVG, Monteiro WM, Pimenta PFP. Microanatomy of the American Malaria Vector Anopheles aquasalis (Diptera: Culicidae: Anophelinae) Midgut: Ultrastructural and Histochemical Observations. Journal Of Medical Entomology 2019, 56: 1636-1649. PMID: 31321415, PMCID: PMC6821279, DOI: 10.1093/jme/tjz114.
- Differential responses of epithelial cells from urinary and biliary tract to eggs of Schistosoma haematobium and S. mansoniNacif-Pimenta R, da Silva Orfanó A, Mosley IA, Karinshak SE, Ishida K, Mann VH, Coelho PMZ, da Costa JMC, Hsieh MH, Brindley PJ, Rinaldi G. Differential responses of epithelial cells from urinary and biliary tract to eggs of Schistosoma haematobium and S. mansoni. Scientific Reports 2019, 9: 10731. PMID: 31341177, PMCID: PMC6656753, DOI: 10.1038/s41598-019-46917-y.
- Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) Delays Egg Hatching and Larval Development of ProgenyChaves BA, Vieira A, Silveira KRD, da Costa Paz A, da Costa Vaz E, Araujo RGP, Rodrigues NB, Campolina TB, da Silva Orfano A, Nacif-Pimenta R, Villegas LEM, de Melo F, de Mello Silva B, Monteiro WM, Guerra M, de Lacerda M, Norris DE, Secundino NFC, Pimenta PFP. Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) Delays Egg Hatching and Larval Development of Progeny. Journal Of Medical Entomology 2019, 56: 1739-1744. PMID: 31278737, PMCID: PMC6821368, DOI: 10.1093/jme/tjz110.
- The Midgut Muscle Network of Anopheles aquasalis (Culicidae, Anophelinae): Microanatomy and Structural Modification After Blood Meal and Plasmodium vivax (Haemosporida, Plasmodiidae) Infection.Baia-da-Silva DC, Orfanó AS, Nacif-Pimenta R, de Melo FF, Simões S, Cabral I, Lacerda MVG, Guerra MDGB, Monteiro WM, Secundino NFC, Pimenta PFP. The Midgut Muscle Network of Anopheles aquasalis (Culicidae, Anophelinae): Microanatomy and Structural Modification After Blood Meal and Plasmodium vivax (Haemosporida, Plasmodiidae) Infection. Journal Of Medical Entomology 2019, 56: 421-431. PMID: 30508123, DOI: 10.1093/jme/tjy199.
- Growth arrested live-attenuated Leishmania infantum KHARON1 null mutants display cytokinesis defect and protective immunity in mice.Santi AMM, Lanza JS, Tunes LG, Fiuza JA, Roy G, Orfanó ADS, de Carvalho AT, Frézard F, Barros ALB, Murta SMF, do Monte-Neto RL. Growth arrested live-attenuated Leishmania infantum KHARON1 null mutants display cytokinesis defect and protective immunity in mice. Scientific Reports 2018, 8: 11627. PMID: 30072701, PMCID: PMC6072785, DOI: 10.1038/s41598-018-30076-7.
- Coinfection with Zika Virus (ZIKV) and Dengue Virus Results in Preferential ZIKV Transmission by Vector Bite to Vertebrate Host.Chaves BA, Orfano AS, Nogueira PM, Rodrigues NB, Campolina TB, Nacif-Pimenta R, Pires ACAM, Júnior ABV, Paz ADC, Vaz EBDC, Guerra MDGVB, Silva BM, de Melo FF, Norris DE, de Lacerda MVG, Pimenta PFP, Secundino NFC. Coinfection with Zika Virus (ZIKV) and Dengue Virus Results in Preferential ZIKV Transmission by Vector Bite to Vertebrate Host. The Journal Of Infectious Diseases 2018, 218: 563-571. PMID: 29659904, PMCID: PMC6047447, DOI: 10.1093/infdis/jiy196.
- The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis.Baia-da-Silva DC, Alvarez LCS, Lizcano OV, Costa FTM, Lopes SCP, Orfanó AS, Pascoal DO, Nacif-Pimenta R, Rodriguez IC, Guerra MDGVB, Lacerda MVG, Secundino NFC, Monteiro WM, Pimenta PFP. The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis. Parasites & Vectors 2018, 11: 148. PMID: 29510729, PMCID: PMC5840820, DOI: 10.1186/s13071-018-2752-5.
- Promising approach to reducing Malaria transmission by ivermectin: Sporontocidal effect against Plasmodium vivax in the South American vectors Anopheles aquasalis and Anopheles darlingi.Pinilla YT, C P Lopes S, S Sampaio V, Andrade FS, Melo GC, Orfanó AS, Secundino NFC, Guerra MGVB, Lacerda MVG, Kobylinski KC, Escobedo-Vargas KS, López-Sifuentes VM, Stoops CA, Baldeviano GC, Tarning J, Vasquez GM, Pimenta PFP, Monteiro WM. Promising approach to reducing Malaria transmission by ivermectin: Sporontocidal effect against Plasmodium vivax in the South American vectors Anopheles aquasalis and Anopheles darlingi. PLoS Neglected Tropical Diseases 2018, 12: e0006221. PMID: 29444080, PMCID: PMC5828505, DOI: 10.1371/journal.pntd.0006221.
- Zika virus infection modulates the bacterial diversity associated with Aedes aegypti as revealed by metagenomic analysis.Villegas LEM, Campolina TB, Barnabe NR, Orfano AS, Chaves BA, Norris DE, Pimenta PFP, Secundino NFC. Zika virus infection modulates the bacterial diversity associated with Aedes aegypti as revealed by metagenomic analysis. PloS One 2018, 13: e0190352. PMID: 29293631, PMCID: PMC5749803, DOI: 10.1371/journal.pone.0190352.
- Bacterial diversity of wild-caught Lutzomyia longipalpis (a vector of zoonotic visceral leishmaniasis in Brazil) under distinct physiological conditions by metagenomics analysis.Pires ACAM, Villegas LEM, Campolina TB, Orfanó AS, Pimenta PFP, Secundino NFC. Bacterial diversity of wild-caught Lutzomyia longipalpis (a vector of zoonotic visceral leishmaniasis in Brazil) under distinct physiological conditions by metagenomics analysis. Parasites & Vectors 2017, 10: 627. PMID: 29284535, PMCID: PMC5747039, DOI: 10.1186/s13071-017-2593-7.
- Zika virus transmission to mouse ear by mosquito bite: a laboratory model that replicates the natural transmission process.Secundino NFC, Chaves BA, Orfano AS, Silveira KRD, Rodrigues NB, Campolina TB, Nacif-Pimenta R, Villegas LEM, Silva BM, Lacerda MVG, Norris DE, Pimenta PFP. Zika virus transmission to mouse ear by mosquito bite: a laboratory model that replicates the natural transmission process. Parasites & Vectors 2017, 10: 346. PMID: 28728607, PMCID: PMC5520231, DOI: 10.1186/s13071-017-2286-2.
- Species-specific escape of Plasmodium sporozoites from oocysts of avian, rodent, and human malarial parasites.Orfano AS, Nacif-Pimenta R, Duarte AP, Villegas LM, Rodrigues NB, Pinto LC, Campos KM, Pinilla YT, Chaves B, Barbosa Guerra MG, Monteiro WM, Smith RC, Molina-Cruz A, Lacerda MV, Secundino NF, Jacobs-Lorena M, Barillas-Mury C, Pimenta PF. Species-specific escape of Plasmodium sporozoites from oocysts of avian, rodent, and human malarial parasites. Malaria Journal 2016, 15: 394. PMID: 27480269, PMCID: PMC4969971, DOI: 10.1186/s12936-016-1451-y.
- Plasmodium yoelii nigeriensis (N67) Is a Robust Animal Model to Study Malaria Transmission by South American Anopheline Mosquitoes.Orfano AS, Duarte AP, Molina-Cruz A, Pimenta PF, Barillas-Mury C. Plasmodium yoelii nigeriensis (N67) Is a Robust Animal Model to Study Malaria Transmission by South American Anopheline Mosquitoes. PloS One 2016, 11: e0167178. PMID: 27911924, PMCID: PMC5135088, DOI: 10.1371/journal.pone.0167178.
- An overview of malaria transmission from the perspective of Amazon Anopheles vectors.Pimenta PF, Orfano AS, Bahia AC, Duarte AP, Ríos-Velásquez CM, Melo FF, Pessoa FA, Oliveira GA, Campos KM, Villegas LM, Rodrigues NB, Nacif-Pimenta R, Simões RC, Monteiro WM, Amino R, Traub-Cseko YM, Lima JB, Barbosa MG, Lacerda MV. An overview of malaria transmission from the perspective of Amazon Anopheles vectors. Memórias Do Instituto Oswaldo Cruz 2015, 110: 23-47. PMID: 25742262, PMCID: PMC4371216, DOI: 10.1590/0074-02760140266.
- Schistosoma mansoni in Susceptible and Resistant Snail Strains Biomphalaria tenagophila: In Vivo Tissue Response and In Vitro Hemocyte InteractionsNacif-Pimenta R, de Mattos AC, da Silva Orfanó A, Barbosa L, Pimenta PF, Coelho PM. Schistosoma mansoni in Susceptible and Resistant Snail Strains Biomphalaria tenagophila: In Vivo Tissue Response and In Vitro Hemocyte Interactions. PLOS ONE 2012, 7: e45637. PMID: 23049828, PMCID: PMC3458097, DOI: 10.1371/journal.pone.0045637.
- The JAK-STAT pathway controls Plasmodium vivax load in early stages of Anopheles aquasalis infection.Bahia AC, Kubota MS, Tempone AJ, Araújo HR, Guedes BA, Orfanó AS, Tadei WP, Ríos-Velásquez CM, Han YS, Secundino NF, Barillas-Mury C, Pimenta PF, Traub-Csekö YM. The JAK-STAT pathway controls Plasmodium vivax load in early stages of Anopheles aquasalis infection. PLoS Neglected Tropical Diseases 2011, 5: e1317. PMID: 22069502, PMCID: PMC3206008, DOI: 10.1371/journal.pntd.0001317.