2017
Determination of Human Hepatic CYP2C8 and CYP1A2 Age-Dependent Expression to Support Human Health Risk Assessment for Early Ages
Song G, Sun X, Hines R, McCarver D, Lake B, Osimitz T, Creek M, Clewell H, Yoon M. Determination of Human Hepatic CYP2C8 and CYP1A2 Age-Dependent Expression to Support Human Health Risk Assessment for Early Ages. Drug Metabolism And Disposition 2017, 45: dmd.116.074583. PMID: 28228413, DOI: 10.1124/dmd.116.074583.Peer-Reviewed Original ResearchConceptsCYP2C8 expressionOntogeny dataMonths postnatal ageProtein levelsLiver microsomal samplesAge-dependent expressionMultiple cytochrome P450Weeks' gestationPostnatal agePostnatal dayYoung infantsPostnatal samplesFetal samplesMicrosomal samplesCYP1A2 expressionQuantitative Western blottingPyrethroid metabolismCYP2C8Western blottingHealth risk assessmentHuman health risk assessmentRisk assessmentAgeCarboxylesterase enzymesCytochrome P450
2003
Genetics and Susceptibility to Toxic Chemicals: Do You (or Should You) Know Your Genetic Profile?
Lash L, Hines R, Gonzalez F, Zacharewski T, Rothstein M. Genetics and Susceptibility to Toxic Chemicals: Do You (or Should You) Know Your Genetic Profile? Journal Of Pharmacology And Experimental Therapeutics 2003, 305: 403-409. PMID: 12606672, DOI: 10.1124/jpet.102.039925.Peer-Reviewed Original ResearchConceptsSpecific enzyme systemsExperimental Biology meetingTransgenic technologyGenetic determinantsEnzyme systemGenetic profileToxic chemicalsGeneticsDivision of ToxicologyComplex interplayHuman healthIndividual susceptibilityPharmacogenomicsExperimental therapeuticsPhysiologySusceptibilityChemicalsDivisionTherapeutics
2002
GENETIC POLYMORPHISMS OF FLAVIN-CONTAINING MONOOXYGENASE (FMO)
Krueger S, Williams D, Yueh M, Martin S, Hines R, Raucy J, Dolphin C, Shephard E, Phillips I. GENETIC POLYMORPHISMS OF FLAVIN-CONTAINING MONOOXYGENASE (FMO). Drug Metabolism Reviews 2002, 34: 523-532. PMID: 12214664, DOI: 10.1081/dmr-120005653.Peer-Reviewed Original ResearchConceptsFlavin-Containing MonooxygenaseAdult human liverPercent of individualsPlethora of drugsTrimethylamine N-oxygenationDrug metabolismHuman liverGenetic polymorphismsHispanic descentPremature stop codonPolymorphic expressionLungPreliminary evidenceFunctional FMO2African descentXenobiotic toxicityMammalian flavinN-oxygenationFMO2TrimethylaminuriaEthylene thioureaPolymorphismAllelesPatientsExpression
2001
Molecular regulation of genes encoding xenobiotic-metabolizing enzymes: mechanisms involving endogenous factors.
Hines R, Luo Z, Cresteil T, Ding X, Prough R, Fitzpatrick J, Ripp S, Falkner K, Ge N, Levine A, Elferink C. Molecular regulation of genes encoding xenobiotic-metabolizing enzymes: mechanisms involving endogenous factors. Drug Metabolism And Disposition 2001, 29: 623-33. PMID: 11302926.Peer-Reviewed Original ResearchConceptsDevelopmental-specific expressionSignal transduction pathwaysMolecular regulationTransduction pathwaysCommon signal transduction pathwayReceptor-mediated signal transduction pathwaysRegulation of xenobioticGenome levelTranscription factorsMolecular mechanismsMolecular biologyEndogenous factorsGenesRegulatory systemXenobiotic-metabolizing enzymesEnzymeRegulationExpressionCytochrome P450Fundamental rolePathwayRapid proliferationTissueBiologyExperimental therapeutics
1995
Developmental regulation of flavin-containing monooxygenase (FMO) isoforms 1 and 2 in pregnant rabbit
Lee M, Smiley S, Kadkhodayan S, Hines R, Williams D. Developmental regulation of flavin-containing monooxygenase (FMO) isoforms 1 and 2 in pregnant rabbit. Chemico-Biological Interactions 1995, 96: 75-85. PMID: 7720106, DOI: 10.1016/0009-2797(94)03584-u.Peer-Reviewed Original ResearchConceptsCortisol levelsRabbit lungsClassical cytochrome P450 inducersPeaks of progesteroneCytochrome P450 inducersPlasma cortisol levelsMaternal kidneyPregnant rabbitsPlasma progesteroneP450 inducersProgesteroneLungMRNA levelsHormonal regulationGestationMacrolide antibioticsTime courseKidneyFMO activityLiverAdministrationFMO2Mammalian flavinDiverse xenobioticsNumber of laboratoriesCharacterization of hepatic flavin monooxygenase from the marine teleost turbot (Scophthalmus maximus L.)
Peters L, Livingstone D, Shenin-Johnson S, Hines R, Schlenk D. Characterization of hepatic flavin monooxygenase from the marine teleost turbot (Scophthalmus maximus L.). Xenobiotica 1995, 25: 121-131. PMID: 7618340, DOI: 10.3109/00498259509061838.Peer-Reviewed Original ResearchConceptsFlavin monooxygenaseNADPH-dependent flavin monooxygenaseFMO-1Full-length cDNA probeFMO-2TMA N-oxideGene expressionMarine teleostsMature fishNorthern analysisCDNA probeXenobiotic metabolismDMA N-oxideImmunoreactive proteinOsmoregulationConversion of trimethylamineN-oxidase activityMonooxygenaseMichaelis-Menten kineticsSingle bandTurbotPiperonyl butoxideTeleostsFishProtein
1994
The Mammalian Flavin-Containing Monooxygenases: Molecular Characterization and Regulation of Expression
Hines R, Cashman J, Philpot R, Williams D, Ziegler D. The Mammalian Flavin-Containing Monooxygenases: Molecular Characterization and Regulation of Expression. Toxicology And Applied Pharmacology 1994, 125: 1-6. PMID: 8128486, DOI: 10.1006/taap.1994.1042.Peer-Reviewed Original ResearchConceptsFlavin-containing monooxygenaseRegulation of expressionSpecies-specific differencesFlavin-Containing MonooxygenasesMixture of cisSubstrate specificityMammalian speciesToxicant susceptibilitySoft nucleophilesMolecular characterizationEnzyme's abilitySecondary alkylMultiple tissuesEarly stepsActive derivativesEnzyme systemUnique stereoselectivityFlavin enzymesHuman FMO3Toxic intermediatesFMO expressionThiocarbamideSpeciesChemical toxicityCytochrome P450