2017
Genetic Evidence for Erythrocyte Receptor Glycophorin B Expression Levels Defining a Dominant Plasmodium falciparum Invasion Pathway into Human Erythrocytes
Dankwa S, Chaand M, Kanjee U, Jiang RHY, Nobre LV, Goldberg JM, Bei AK, Moechtar MA, Grüring C, Ahouidi AD, Ndiaye D, Dieye TN, Mboup S, Weekes MP, Duraisingh MT. Genetic Evidence for Erythrocyte Receptor Glycophorin B Expression Levels Defining a Dominant Plasmodium falciparum Invasion Pathway into Human Erythrocytes. Infection And Immunity 2017, 85: 10.1128/iai.00074-17. PMID: 28760933, PMCID: PMC5607420, DOI: 10.1128/iai.00074-17.Peer-Reviewed Original ResearchConceptsInvasion pathwaysGlycophorin CGlycophorin BExpression levelsShort hairpin RNA transductionLigand-receptor interactionsErythroid progenitor cellsKnockout linesGenetic evidenceBioinformatics analysisKnockdown cellsProteomic profilingTranscript levelsMultiple proteinsHuman erythrocytesExtensive variationParasite invasionProgenitor cellsInvasion ligandsB expression levelsLaboratory strainsGPC receptorsCulture-adapted strainsPathwayReceptor usage
2010
A flow cytometry‐based assay for measuring invasion of red blood cells by Plasmodium falciparum
Bei AK, DeSimone TM, Badiane AS, Ahouidi AD, Dieye T, Ndiaye D, Sarr O, Ndir O, Mboup S, Duraisingh MT. A flow cytometry‐based assay for measuring invasion of red blood cells by Plasmodium falciparum. American Journal Of Hematology 2010, 85: 234-237. PMID: 20196166, PMCID: PMC3089760, DOI: 10.1002/ajh.21642.Peer-Reviewed Original ResearchConceptsParasite invasionMalaria parasite Plasmodium falciparumParasite Plasmodium falciparumPlasmodium falciparumFlow cytometry-based assayParasite multiplication rateFlow cytometry-based methodParasite developmentCytometry-based assayCytometry-based methodRed blood cellsDisease severityDye SYBR Green IRing stageVariable invasionMultiplication rateBlood cellsInvasionSYBR Green IHuman erythrocytesFalciparumGreen IImportant determinantErythrocytesParasitemia
2009
Erythrocyte invasion profiles are associated with a common invasion ligand polymorphism in Senegalese isolates of Plasmodium falciparum
LANTOS PM, AHOUIDI AD, BEI AK, JENNINGS CV, SARR O, NDIR O, WIRTH DF, MBOUP S, DURAISINGH MT. Erythrocyte invasion profiles are associated with a common invasion ligand polymorphism in Senegalese isolates of Plasmodium falciparum. Parasitology 2009, 136: 1-9. PMID: 19126266, DOI: 10.1017/s0031182008005167.Peer-Reviewed Original ResearchConceptsErythrocyte receptorSialic acid-independent invasionBlood group O patientsDifferent erythrocyte receptorsGroup O patientsSenegalese isolatesParasite multiplication ratePlasmodium falciparum parasitesMultiple ligand-receptor interactionsO patientsFalciparum parasitesLarge sequence deletionsVariant expression levelParasite isolatesPlasmodium falciparumErythrocyte invasionSelectivity indexReceptorsExpression levelsMajor polymorphismsInvasionInvasion pathwaysPossible determinantsParasitesHuman erythrocytes