2015
Dead enzymes in the aldehyde dehydrogenase gene family: role in drug metabolism and toxicology
Jackson BC, Thompson DC, Charkoftaki G, Vasiliou V. Dead enzymes in the aldehyde dehydrogenase gene family: role in drug metabolism and toxicology. Expert Opinion On Drug Metabolism & Toxicology 2015, 11: 1839-1847. PMID: 26558415, PMCID: PMC4937717, DOI: 10.1517/17425255.2016.1108406.Peer-Reviewed Original ResearchConceptsAldehyde dehydrogenase gene familiesDehydrogenase gene familyDead enzymesGene familyActive enzymeProtein-protein interactionsTotal enzyme populationNon-enzymatic functionsALDH proteinsAvailability of substratesSubcellular spaceGene productsKey residuesProtein recordsCatalytic activityEnzyme populationPathophysiological functionsAllosteric modulationEnzymeBiological actionsProteinBiological activityComputational analysisALDHFamily
2012
ALDH1A Isozymes are Markers of Human Melanoma Stem Cells and Potential Therapeutic Targets
Luo Y, Dallaglio K, Chen Y, Robinson WA, Robinson SE, McCarter MD, Wang J, Gonzalez R, Thompson DC, Norris DA, Roop DR, Vasiliou V, Fujita M. ALDH1A Isozymes are Markers of Human Melanoma Stem Cells and Potential Therapeutic Targets. Stem Cells 2012, 30: 2100-2113. PMID: 22887839, PMCID: PMC3448863, DOI: 10.1002/stem.1193.Peer-Reviewed Original ResearchMeSH KeywordsAldehyde DehydrogenaseAldehyde Dehydrogenase 1 FamilyAldehyde OxidoreductasesAnimalsApoptosisCell Transformation, NeoplasticDacarbazineDrug Resistance, NeoplasmFemaleGene Expression Regulation, NeoplasticGene SilencingHumansIsoenzymesMelanomaMiceMice, Inbred NODMice, SCIDNeoplasm TransplantationNeoplastic Stem CellsResponse ElementsRetinal DehydrogenaseRNA, Small InterferingSkin NeoplasmsTemozolomideTretinoinConceptsCancer stem cellsPositive melanoma cellsMelanoma cellsTherapeutic targetBiomarkers of CSCsHuman melanomaPatient-derived tumor specimensMelanoma cancer stem cellsNOD/SCID miceALDH-negative cellsHigh aldehyde dehydrogenase (ALDH) activityALDH isozymesNonobese diabetic/Potential therapeutic targetDrug-induced cell deathAttractive therapeutic targetNew molecular targetsHuman melanoma cellsStem cellsMelanoma stem cellsAldehyde dehydrogenase activityHuman melanoma stem cellsNSG miceCell cycle arrestImmunodeficiency miceCharacterization of aldehyde dehydrogenase isozymes in ovarian cancer tissues and sphere cultures
Saw YT, Yang J, Ng SK, Liu S, Singh S, Singh M, Welch WR, Tsuda H, Fong WP, Thompson D, Vasiliou V, Berkowitz RS, Ng SW. Characterization of aldehyde dehydrogenase isozymes in ovarian cancer tissues and sphere cultures. BMC Cancer 2012, 12: 329. PMID: 22852552, PMCID: PMC3458927, DOI: 10.1186/1471-2407-12-329.Peer-Reviewed Original ResearchConceptsClear cell tumorsWestern blot analysisCell tumorsOvarian tissueALDH isozymesCancer cellsClear cell subtypeSphere cultureOvarian cancer tissuesQuantitative reverse transcription polymerase chain reactionNormal ovarian tissuesOvarian epithelial tumorsReverse transcription-polymerase chain reactionHuman ovarian cancerOvarian tumor tissuesTranscription-polymerase chain reactionOvarian cancer cellsBlot analysisHuman cancer stem cellsCancer stem cellsOvarian cancer pathophysiologyMRNA expression levelsResultsImmunohistochemical stainingCancer cell linesMucinous tumors