2010
Construction and maintenance of randomized retroviral expression libraries for transmembrane protein engineering
Marlatt SA, Kong Y, Cammett TJ, Korbel G, Noonan JP, DiMaio D. Construction and maintenance of randomized retroviral expression libraries for transmembrane protein engineering. Protein Engineering Design And Selection 2010, 24: 311-320. PMID: 21149273, PMCID: PMC3038463, DOI: 10.1093/protein/gzq112.Peer-Reviewed Original ResearchConceptsSignificant genetic bottleneckRetroviral expression libraryDeep DNA sequencingAmino acid segmentNovel biological activitiesGenetic bottleneckTransmembrane domainMammalian cellsLibrary sequencesRandom mutagenesisProtein engineeringExpression libraryDeep sequencingDifferent proteinsSmall proteinsGenetic selectionAcid segmentRetroviral libraryDNA sequencingSequencing resultsRandomized libraryProteinPowerful approachSequencingDiversity
2009
Calculating complexity of large randomized libraries
Kong Y. Calculating complexity of large randomized libraries. Journal Of Theoretical Biology 2009, 259: 641-645. PMID: 19376134, DOI: 10.1016/j.jtbi.2009.04.008.Peer-Reviewed Original Research