2010
Direct inhibition of hypocretin/orexin neurons in the lateral hypothalamus by nociceptin/orphanin FQ blocks stress-induced analgesia in rats
Gerashchenko D, Horvath TL, Xie X. Direct inhibition of hypocretin/orexin neurons in the lateral hypothalamus by nociceptin/orphanin FQ blocks stress-induced analgesia in rats. Neuropharmacology 2010, 60: 543-549. PMID: 21195099, PMCID: PMC3031765, DOI: 10.1016/j.neuropharm.2010.12.026.Peer-Reviewed Original ResearchMeSH KeywordsAnalgesiaAnalysis of VarianceAnimalsCell CountHypothalamic Area, LateralImmunohistochemistryIntracellular Signaling Peptides and ProteinsMaleMicroinjectionsMicroscopy, ElectronNeuronsNeuropeptidesOpioid PeptidesOrexinsPain MeasurementPain PerceptionProto-Oncogene Proteins c-fosRatsRats, Sprague-DawleyReceptors, OpioidRestraint, PhysicalStatistics, NonparametricStress, PhysiologicalConceptsStress-induced analgesiaHcrt neuronsLateral hypothalamusPerifornical areaNociceptin/orphanin FQ systemHypocretin/orexin neuronsNociceptin/orphanin FQHcrt neuronal activityDirect inhibitionThermal pain thresholdThermal nociceptive testsHypocretin/orexinFos immunohistochemistryOrexin neuronsBilateral microinjectionIntracerebroventricular injectionPain thresholdNociceptive testsOrphanin FQOFQ receptorMouse modelNeuronal activityBrain areasHypothalamusNeuronsCorticosterone Regulates Synaptic Input Organization of POMC and NPY/AgRP Neurons in Adult Mice
Gyengesi E, Liu ZW, D'Agostino G, Gan G, Horvath TL, Gao XB, Diano S. Corticosterone Regulates Synaptic Input Organization of POMC and NPY/AgRP Neurons in Adult Mice. Endocrinology 2010, 151: 5395-5402. PMID: 20843996, PMCID: PMC2954711, DOI: 10.1210/en.2010-0681.Peer-Reviewed Original ResearchConceptsNPY/AgRP neuronsSynaptic input organizationSham-operated controlsAgRP neuronsADX miceCorticosterone replacementNeuropeptide YArcuate nucleusSynaptic arrangementsInput organizationPair-fed control animalsPutative excitatory synapsesPutative inhibitory synapsesHypothalamic arcuate nucleusEffects of adrenalectomySham-operated animalsNumber of synapsesPOMC neuronsProtein (AgRP) neuronsOrexigenic actionMembrane potentialSynaptic analysisInhibitory synapsesExcitatory synapsesFood intakeEnhanced anorexigenic signaling in lean obesity resistant syndecan-3 null mice
Zheng Q, Zhu J, Shanabrough M, Borok E, Benoit SC, Horvath TL, Clegg DJ, Reizes O. Enhanced anorexigenic signaling in lean obesity resistant syndecan-3 null mice. Neuroscience 2010, 171: 1032-1040. PMID: 20923696, PMCID: PMC2991621, DOI: 10.1016/j.neuroscience.2010.09.060.Peer-Reviewed Original ResearchMeSH KeywordsAgouti-Related ProteinAlpha-MSHAnalysis of VarianceAnimalsBody WeightDose-Response Relationship, DrugEatingFeeding BehaviorFood DeprivationGene Expression RegulationMaleMiceMice, KnockoutNeuronsNeuropeptide YParaventricular Hypothalamic NucleusProto-Oncogene Proteins c-fosSignal TransductionSyndecan-3Time FactorsConceptsMelanocortin agonist melanotan IISyndecan-3 null miceParaventricular nucleusBody weightNull miceHypothalamic target neuronsNeuropeptide α-MSHRisk of diabetesC-Fos immunoreactivityHypothalamic paraventricular nucleusBody weight regulationWild-type miceTypes of cancerAnorexigenic αAgRP neuronsHormone neuronsHypothalamic circuitsNeuropeptide YAnorexigenic signalingNeuropeptide responsesCardiovascular diseaseFood intakeTarget neuronsMelanotan IIType miceEarly-Life Experience Reduces Excitation to Stress-Responsive Hypothalamic Neurons and Reprograms the Expression of Corticotropin-Releasing Hormone
Korosi A, Shanabrough M, McClelland S, Liu ZW, Borok E, Gao XB, Horvath TL, Baram TZ. Early-Life Experience Reduces Excitation to Stress-Responsive Hypothalamic Neurons and Reprograms the Expression of Corticotropin-Releasing Hormone. Journal Of Neuroscience 2010, 30: 703-713. PMID: 20071535, PMCID: PMC2822406, DOI: 10.1523/jneurosci.4214-09.2010.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnalysis of VarianceAnimalsAnimals, NewbornChromatin ImmunoprecipitationCorticotropin-Releasing HormoneExcitatory Amino Acid AntagonistsFemaleGene Expression Regulation, DevelopmentalMaleMaternal DeprivationMicroscopy, Electron, TransmissionNeuronsParaventricular Hypothalamic NucleusPatch-Clamp TechniquesPhysical StimulationPregnancyRatsRats, Sprague-DawleyRepressor ProteinsRNA, MessengerSodium Channel BlockersStress, PsychologicalSynaptic PotentialsTetrodotoxinVesicular Glutamate Transport Protein 2ConceptsCorticotropin-releasing hormoneNeuron-restrictive silencer factorCRH neuronsHypothalamic neuronsCRH expressionEarly life experiencesMiniature excitatory synaptic currentsHypothalamic CRH neuronsExcitatory synaptic currentsCRH gene expressionGlutamate vesicular transporterCRH gene transcriptionTranscriptional repressor neuron-restrictive silencer factorExcitatory innervationExperience-induced neuroplasticityInhibitory synapsesRat pupsExcitatory synapsesSynaptic currentsPersistent suppressionVesicular transportersCognitive functionNeuronsSilencer factorMaternal care
2006
Orexin neuronal changes in the locus coeruleus of the aging rhesus macaque
Downs JL, Dunn MR, Borok E, Shanabrough M, Horvath TL, Kohama SG, Urbanski HF. Orexin neuronal changes in the locus coeruleus of the aging rhesus macaque. Neurobiology Of Aging 2006, 28: 1286-1295. PMID: 16870307, DOI: 10.1016/j.neurobiolaging.2006.05.025.Peer-Reviewed Original ResearchConceptsLateral hypothalamic areaPoor sleep qualityLocus coeruleusNoradrenergic locus coeruleusSleep qualityNeuron numberRhesus macaquesAge-related decreaseTyrosine hydroxylase mRNAAge-related lossMale rhesus macaquesOrexin innervationOrexin neuronsOrexin neuropeptidesAge-related declineB immunoreactivityHypothalamic areaNeuronal changesElderly humansAxon densityLC contributeHydroxylase mRNANonhuman primatesOld animalsAdult animals
2004
Sex differences in adult suprachiasmatic nucleus neurons emerging late prenatally in rats
Abizaid A, Mezei G, Sotonyi P, Horvath TL. Sex differences in adult suprachiasmatic nucleus neurons emerging late prenatally in rats. European Journal Of Neuroscience 2004, 19: 2488-2496. PMID: 15128402, DOI: 10.1111/j.0953-816x.2004.03359.x.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnalysis of VarianceAnimalsAnimals, NewbornArginine VasopressinBromodeoxyuridineCalbindin 1CalbindinsCell CountEmbryo, MammalianFemaleGeniculate BodiesGlial Fibrillary Acidic ProteinImmunohistochemistryMaleNeuronsNeuropeptide YPregnancyPrenatal Exposure Delayed EffectsRatsRats, Sprague-DawleyS100 Calcium Binding Protein GSex CharacteristicsSuprachiasmatic NucleusTestosterone PropionateConceptsSuprachiasmatic nucleusGonadal steroidsFemale ratsLate gestationSex differencesPregnant female ratsVasoactive intestinal peptideGestational day 18Postnatal day 60BrdU-labeled cellsCalbindin-D28KSuprachiasmatic nucleus neuronsCircadian rhythmIntestinal peptideNucleus neuronsDouble immunocytochemistryGonadal functionTestosterone propionateBrain sectionsRat fetusesDay 18Day 60Cell groupsRatsSCN cells
1996
Morphological and pharmacological evidence for neuropeptide Y-galanin interaction in the rat hypothalamus
Horvath TL, Naftolin F, Leranth C, Sahu A, Kalra SP. Morphological and pharmacological evidence for neuropeptide Y-galanin interaction in the rat hypothalamus. Endocrinology 1996, 137: 3069-3077. PMID: 8770933, DOI: 10.1210/endo.137.7.8770933.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsArcuate Nucleus of HypothalamusAxonsCerebral VentriclesColchicineDendritesEstradiolEstrusFemaleGalaninHypothalamusHypothalamus, AnteriorImmunoenzyme TechniquesImmunohistochemistryLuteinizing HormoneMicroscopy, ImmunoelectronNeuronsNeuropeptide YOvariectomyParaventricular Hypothalamic NucleusPreoptic AreaProgesteroneRatsRats, Sprague-DawleySignal TransductionSubstance PSupraoptic NucleusConceptsNeuropeptide YMedial preoptic areaLH releaseGAL neuronsArcuate nucleusIntraventricular injectionParaventricular nucleusPreoptic areaSupraoptic nucleusSynaptic connectionsBasal LH releaseNPY-immunoreactive axonsPituitary hormone secretionPituitary gonadotropin releaseRelease of gonadotropinsMicroscopic examinationHypothalamic peptidergic systemsPeroxidase-antiperoxidase techniqueAvidin-biotin peroxidaseLight microscopic examinationMechanism of actionDiaminobenzidine reactionGAL releaseNPY inputNPY administration