25 Electron Microscopic Double and Triple Labeling Immunocytochemistry in Elucidation of Synaptological Interactions between Ovarian Steroid-Sensitive Neurons and Circuits
Leranth C, Naftolin F, Shanabrough M, Horvath T. 25 Electron Microscopic Double and Triple Labeling Immunocytochemistry in Elucidation of Synaptological Interactions between Ovarian Steroid-Sensitive Neurons and Circuits. International Review Of Neurobiology 1994, 22: 403-434. DOI: 10.1016/b978-0-12-185292-4.50031-0.Peer-Reviewed Original ResearchDouble immunostaining techniqueHormone-releasing hormone (LHRH) neuronsSteroid-sensitive neuronsImmunostaining techniquesRetrograde tracer techniqueTriple-labeling immunocytochemistryHormone neuronsDouble immunostainingTissue antigensDifferent anestheticsAnatomical basisSame satisfactory resultsTissue preparationsNeuronsElectron microscopicElectron-dense labelKetaminePentobarbitalAnestheticsImmunostainingAntigenDegenerationMethoxyfluraneChloropentAntibodies