2024
Noninvasive in vivo photoacoustic detection of malaria with Cytophone in Cameroon
Yadem A, Armstrong J, Sarimollaoglu M, Kiki Massa C, Ndifo J, Menyaev Y, Mbe A, Richards K, Wade M, Zeng Y, Chen R, Zhou Q, Meten E, Ntone R, Tchuedji Y, Ullah S, Galanzha E, Eteki L, Gonsu H, Biris A, Suen J, Boum Y, Zharov V, Parikh S. Noninvasive in vivo photoacoustic detection of malaria with Cytophone in Cameroon. Nature Communications 2024, 15: 9228. PMID: 39455558, PMCID: PMC11511992, DOI: 10.1038/s41467-024-53243-z.Peer-Reviewed Original ResearchConceptsClearance of parasitemiaDetection of malariaMalaria-infected red blood cellsDiagnosed symptomatic casesCross-sectional cohortUncomplicated malariaMalaria diagnosticsMalaria infectionRed blood cellsSymptomatic casesTarget antigenAsymptomatic reservoirCameroonian adultsFlow cytometer platformBlood samplesReservoir of infectionBlood cellsLack sensitivityLongitudinal cohortMolecular assaysMalariaIRBCPoint-of-careCohortQuantitative PCRPersistent and multiclonal malaria parasite dynamics despite extended artemether-lumefantrine treatment in children
Goodwin J, Kajubi R, Wang K, Li F, Wade M, Orukan F, Huang L, Whalen M, Aweeka F, Mwebaza N, Parikh S. Persistent and multiclonal malaria parasite dynamics despite extended artemether-lumefantrine treatment in children. Nature Communications 2024, 15: 3817. PMID: 38714692, PMCID: PMC11076639, DOI: 10.1038/s41467-024-48210-7.Peer-Reviewed Original ResearchConceptsDay 7 lumefantrine concentrationsArtemether-lumefantrine treatmentRing-stage parasitesEarly post-treatmentEarly post-treatment periodArtemether-lumefantrineArtemisinin resistanceDay regimenMulticlonal infectionsEfficacious therapyFollow-upRandomized trialsPersistent clonesTransmission settingsEffective treatmentPost-treatment periodRegimensAntimalarial studiesStandard diagnosticsStandard 3DaysPost-treatmentChildrenTreatmentTherapy
2023
Tracking antimalarial drug resistance using mosquito blood meals: a cross-sectional study
Ehrlich H, Somé A, Bazié T, Ebou C, Dembélé E, Balma R, Goodwin J, Wade M, Bei A, Ouédraogo J, Foy B, Dabiré R, Parikh S. Tracking antimalarial drug resistance using mosquito blood meals: a cross-sectional study. The Lancet Microbe 2023, 4: e461-e469. PMID: 37086737, PMCID: PMC10365133, DOI: 10.1016/s2666-5247(23)00063-0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntimalarialsCross-Sectional StudiesCulicidaeFolic Acid AntagonistsHumansPlasmodium falciparumPolymerase Chain ReactionConceptsMosquito blood mealsAntimalarial drug resistanceSurvey 3Blood-fed mosquitoesBlood samplesSurvey 1Survey 2Blood mealDrug resistanceUltrasensitive quantitative PCRHuman blood samplesCross-sectional studyMargin of equivalenceStrong surveillance systemCross-sectional surveySupplementary Materials sectionMarker of clonalityPragmatic thresholdAntimalarial resistanceDrug susceptibilityInfectious diseasesPlasmodium falciparumNational InstituteTolerabilityMaterial section
2022
Clinical characteristics of Plasmodium falciparum infection among symptomatic patients presenting to a major urban military hospital in Cameroon
Hodson DZ, Mbarga Etoundi Y, Mbatou Nghokeng N, Mohamadou Poulibe R, Magne Djoko S, Goodwin J, Cheteug Nguesta G, Nganso T, Armstrong JN, Andrews JJ, Zhang E, Wade M, Eboumbou Moukoko CE, Boum Y, Parikh S. Clinical characteristics of Plasmodium falciparum infection among symptomatic patients presenting to a major urban military hospital in Cameroon. Malaria Journal 2022, 21: 298. PMID: 36273147, PMCID: PMC9588226, DOI: 10.1186/s12936-022-04315-2.Peer-Reviewed Original ResearchConceptsP. falciparum infectionPopulation attributable risk percentFalciparum infectionPlasmodium falciparum infectionYears of ageClinical characteristicsUrban hospitalMilitary HospitalAttributable risk percentHigher positivity rateLikelihood ratioRapid diagnostic testsMajor urban hospitalRural African settingConclusionsThe prevalenceHigh feverSymptomatic patientsHemoglobin levelsAnemia prevalenceSevere anemiaEmergency departmentVenous samplesClinical surveyPositivity rateWHO definitionTowards rainbow portable Cytophone with laser diodes for global disease diagnostics
Jawad H, Yadem A, Menyaev Y, Sarimollaoglu M, Armstrong J, Watanabe F, Biris A, Stumhofer J, Nedosekin D, Suen J, Parikh S, Zharov V. Towards rainbow portable Cytophone with laser diodes for global disease diagnostics. Scientific Reports 2022, 12: 8671. PMID: 35606373, PMCID: PMC9126638, DOI: 10.1038/s41598-022-11452-w.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsEarly Detection of CancerErythrocytesLasers, SemiconductorMalariaMelanomaMicePlasmodium falciparumPlasmodium yoeliiConceptsPlasmodium yoelii murine modelDetect infected cellsMalaria-infected erythrocytesDiagnosis of melanomaMalaria progressionSickle anemiaMurine modelDiagnosis of cancerMelanoma cellsEarly diagnosisCardiovascular disordersIn vivoEarly diagnosis of cancerInfected cellsIn vitroBlood backgroundDisease markersRed clotsMelanomaHemozoinMarkersSelectable markerBacteremiaCellsAnemia
2021
Mapping partner drug resistance to guide antimalarial combination therapy policies in sub-Saharan Africa
Ehrlich HY, Bei AK, Weinberger DM, Warren JL, Parikh S. Mapping partner drug resistance to guide antimalarial combination therapy policies in sub-Saharan Africa. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2100685118. PMID: 34261791, PMCID: PMC8307356, DOI: 10.1073/pnas.2100685118.Peer-Reviewed Original Research
2016
Malaria in HIV-Infected Children Receiving HIV Protease-Inhibitor- Compared with Non-Nucleoside Reverse Transcriptase Inhibitor-Based Antiretroviral Therapy, IMPAACT P1068s, Substudy to P1060
Hobbs CV, Gabriel EE, Kamthunzi P, Tegha G, Tauzie J, Petzold E, Barlow-Mosha L, H. BH, Li Y, Ilmet T, Kirmse B, Neal J, Parikh S, Deygoo N, Philippe P, Mofenson L, Prescott W, Chen J, Musoke P, Palumbo P, Duffy PE, Borkowsky W, Team F. Malaria in HIV-Infected Children Receiving HIV Protease-Inhibitor- Compared with Non-Nucleoside Reverse Transcriptase Inhibitor-Based Antiretroviral Therapy, IMPAACT P1068s, Substudy to P1060. PLOS ONE 2016, 11: e0165140. PMID: 27936233, PMCID: PMC5147802, DOI: 10.1371/journal.pone.0165140.Peer-Reviewed Original ResearchMeSH KeywordsAntimalarialsCD4 Lymphocyte CountChildChild, PreschoolCoinfectionDrug Therapy, CombinationFemaleHIV InfectionsHIV Protease InhibitorsHIV-1HumansInfantLamivudineLopinavirMalaria, FalciparumMalawiMaleNevirapinePlasmodium falciparumReverse Transcriptase InhibitorsRitonavirViral LoadZidovudineConceptsNon-nucleoside reverse transcriptase inhibitorAntiretroviral therapyReverse transcriptase inhibitorBlood smear microscopyHIV protease inhibitorsPositive BSTranscriptase inhibitorProtease inhibitorsClinical malaria incidenceMalaria parasite carriageMalaria-endemic settingsHIV antiretroviral therapyAnti-malarial treatmentLopinavir-ritonavirIllness visitsParasite carriageMalaria treatmentClinical studiesSmear microscopyLower riskMalaria incidenceFurther evaluationMalaria parasitesHIVMonthsPopulation Pharmacokinetics and Pharmacodynamics of Lumefantrine in Young Ugandan Children Treated With Artemether-Lumefantrine for Uncomplicated Malaria
Tchaparian E, Sambol NC, Arinaitwe E, McCormack SA, Bigira V, Wanzira H, Muhindo M, Creek DJ, Sukumar N, Blessborn D, Tappero JW, Kakuru A, Bergqvist Y, Aweeka FT, Parikh S. Population Pharmacokinetics and Pharmacodynamics of Lumefantrine in Young Ugandan Children Treated With Artemether-Lumefantrine for Uncomplicated Malaria. The Journal Of Infectious Diseases 2016, 214: 1243-1251. PMID: 27471317, PMCID: PMC5034953, DOI: 10.1093/infdis/jiw338.Peer-Reviewed Original ResearchConceptsArtemether-lumefantrineRecurrent parasitemiaLumefantrine exposurePopulation pharmacokineticsUgandan childrenYoung Ugandan childrenPlasmodium falciparum malariaDay 7 concentrationsAge 6 monthsFirst-order absorptionWhole blood concentrationsYoung childrenUncomplicated malariaFalciparum malariaBlood concentrationsTreatment outcomesLumefantrine concentrationsSignificant positive correlationParasitemiaOlder childrenPharmacokineticsLumefantrineMalariaExposure levelsOpen model
2009
Intermittent Preventive Therapy for Malaria in Pregnancy: Is Sulfadoxine–Pyrimethamine the Right Drug?
Parikh S, Rosenthal PJ. Intermittent Preventive Therapy for Malaria in Pregnancy: Is Sulfadoxine–Pyrimethamine the Right Drug? Clinical Pharmacology & Therapeutics 2009, 87: 160-162. PMID: 20107451, DOI: 10.1038/clpt.2009.284.Peer-Reviewed Original Research
2005
Antimalarial Activity of Human Immunodeficiency Virus Type 1 Protease Inhibitors
Parikh S, Gut J, Istvan E, Goldberg DE, Havlir DV, Rosenthal PJ. Antimalarial Activity of Human Immunodeficiency Virus Type 1 Protease Inhibitors. Antimicrobial Agents And Chemotherapy 2005, 49: 2983-2985. PMID: 15980379, PMCID: PMC1168637, DOI: 10.1128/aac.49.7.2983-2985.2005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntimalarialsAspartic Acid EndopeptidasesCulture MediaHIV Protease InhibitorsHumansParasitic Sensitivity TestsPlasmodium falciparumConceptsHuman immunodeficiency virus type 1Protease inhibitorsHuman immunodeficiency virus type 1 (HIV-1) protease inhibitorsImmunodeficiency virus type 1HIV-1 protease inhibitorsVirus type 1Antimalarial activityCultured Plasmodium falciparumDevelopment of parasitesLopinavir therapyType 1Malaria parasitesPlasmodium falciparumLopinavirRelevant concentrationsInhibitorsPotent compoundsSimilar concentrationsParasitesRitonavirTherapyActivityFalciparum
2004
Molecular Evaluation of the Natural History of Asymptomatic Parasitemia in Ugandan Children
Nsobya SL, Parikh S, Kironde F, Lubega G, Kamya MR, Rosenthal PJ, Dorsey G. Molecular Evaluation of the Natural History of Asymptomatic Parasitemia in Ugandan Children. The Journal Of Infectious Diseases 2004, 189: 2220-2226. PMID: 15181569, DOI: 10.1086/421281.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsChild, PreschoolGenotypeHumansInfantMalaria, FalciparumMicroscopyParasitemiaPlasmodium falciparumPolymerase Chain ReactionUgandaConceptsSymptomatic malariaPolymerase chain reactionAsymptomatic parasitemiaDetectable parasitemiaNatural historySubsequent clinical malariaClinical malariaAsymptomatic childrenSymptomatic episodesMalarial parasitemiaUgandan childrenPersistent infectionParasitemiaMalariaFirst monthChain reactionMolecular evaluationChildrenHigh ratePrevalenceSimilar ratesMonthsPersistent strainsEpisodesInfection