2001
Regulation of Flavin-Containing Monooxygenase 1 Expression by Ying Yang 1 and Hepatic Nuclear Factors 1 and 4
Luo Z, Hines R. Regulation of Flavin-Containing Monooxygenase 1 Expression by Ying Yang 1 and Hepatic Nuclear Factors 1 and 4. Molecular Pharmacology 2001, 60: 1421-1430. PMID: 11723251, DOI: 10.1124/mol.60.6.1421.Peer-Reviewed Original ResearchAnimalsBase SequenceDNA-Binding ProteinsEnhancer Elements, GeneticErythroid-Specific DNA-Binding FactorsGene Expression Regulation, EnzymologicHepatocyte Nuclear Factor 1Hepatocyte Nuclear Factor 1-alphaHepatocyte Nuclear Factor 1-betaHumansMolecular Sequence DataNuclear ProteinsOxygenasesPromoter Regions, GeneticRabbitsSequence Homology, Nucleic AcidTranscription FactorsTransfectionTumor Cells, CulturedYY1 Transcription Factor
1997
Further Characterization of the Major and Minor RabbitFMO1Promoters and Identification of both Positive and Negative Distal Regulatory Elements
Luo Z, Hines R. Further Characterization of the Major and Minor RabbitFMO1Promoters and Identification of both Positive and Negative Distal Regulatory Elements. Archives Of Biochemistry And Biophysics 1997, 346: 96-104. PMID: 9328289, DOI: 10.1006/abbi.1997.0287.Peer-Reviewed Original ResearchConceptsRegulatory domainRegulatory sequencesRegulatory regionsSpecific DNA/protein complexesDNA/protein complexesUpstream regulatory domainsDistal regulatory elementsNegative regulatory sequencesPositive regulatory domainUpstream regulatory sequencesNegative regulatory regionPositive regulatory regionBasal transcriptional activityProtein complexesTranscription initiationHepG2 nuclear extractsDeletion mutantsMature transcriptsDeletion analysisRegulatory elementsLeader sequenceMinor promoterTransient expressionUpstream promoterTranscriptional activity
1995
Partial Characterization of the Human CYP1A1 Negatively Acting Transcription Factor and Mutational Analysis of Its Cognate DNA Recognition Sequence
Boucher P, Piechocki M, Hines R. Partial Characterization of the Human CYP1A1 Negatively Acting Transcription Factor and Mutational Analysis of Its Cognate DNA Recognition Sequence. Molecular And Cellular Biology 1995, 15: 5144-5151. PMID: 7651431, PMCID: PMC230761, DOI: 10.1128/mcb.15.9.5144.Peer-Reviewed Original ResearchBase SequenceBinding SitesBinding, CompetitiveCells, CulturedCytochrome P-450 Enzyme SystemDNA Mutational AnalysisGene Expression Regulation, EnzymologicHumansMolecular Sequence DataNuclear ProteinsOxygenasesProtein BindingRegulatory Sequences, Nucleic AcidSequence Homology, Nucleic AcidTranscription FactorsIn vitro binding and functional studies comparing the human CYPIAI negative regulatory element with the orthologous sequences from rodent genes
Boucher P, Hines R. In vitro binding and functional studies comparing the human CYPIAI negative regulatory element with the orthologous sequences from rodent genes. Carcinogenesis 1995, 16: 383-392. PMID: 7859371, DOI: 10.1093/carcin/16.2.383.Peer-Reviewed Original ResearchConceptsNegative regulatory elementSpecific DNA-protein complexesDNA-protein complexesTransient expression assaysRegulatory elementsNuclear proteinsMouse fragmentsHeterologous promoterExpression assaysCell linesElectrophoretic mobility shift assaysMouse nuclear proteinMouse CYP1A1 geneSouthwestern blot analysisMobility shift assaysCYP1A1 geneNegative regulatory activitySimilar protection patternMouse hepatoma cell lineRodent cell linesCytosine-rich sequencesCytosine-rich regionsHuman CYP1A1 geneOrthologous sequencesRodent genes
1993
Specific nuclear protein binding to a negative regulatory element on the human CYP1A1 gene
Boucher P, Ruch R, Hines R. Specific nuclear protein binding to a negative regulatory element on the human CYP1A1 gene. Journal Of Biological Chemistry 1993, 268: 17384-17391. PMID: 8349622, DOI: 10.1016/s0021-9258(19)85346-2.Peer-Reviewed Original ResearchConceptsNegative regulatory activityNuclear proteinsElectrophoretic mobility shift assaysCellular trans-acting factorsTrans-acting factorsRegulatory activityStrand-specific mannerCo-transfection studiesMethylation interference experimentsMobility shift assaysNegative regulatory elementSpecific nuclear proteinCytosine-rich sequencesHuman CYP1A1 genePromoter/enhancerRegulatory elementsShift assaysTransient expressionReporter geneDNase IChimeric plasmidsCYP1A1 promoterImportant sequencesCYP1A1 genePalindromes