Regulation of DHP receptor expression by elements in the 5′-flanking sequence
Liu L, Fan Q, El-Zaru M, Vanderpool K, Hines R, Marsh J. Regulation of DHP receptor expression by elements in the 5′-flanking sequence. AJP Heart And Circulatory Physiology 2000, 278: h1153-h1162. PMID: 10749709, DOI: 10.1152/ajpheart.2000.278.4.h1153.Peer-Reviewed Original ResearchMeSH Keywords5' Untranslated Regions8-Bromo Cyclic Adenosine MonophosphateAdrenergic alpha-AgonistsAdrenergic beta-AgonistsAnimalsBase SequenceCalcium Channels, L-TypeCells, CulturedElectrophoresisGene ExpressionGenes, ReporterGenetic Complementation TestGonadal Steroid HormonesIn Vitro TechniquesIsoproterenolLuciferasesMolecular Sequence DataMuscle Fibers, SkeletalMuscle, Smooth, VascularMyocardiumPhenylephrinePromoter Regions, GeneticRatsRNA, MessengerTestosteroneTranscription, GeneticTransfectionConceptsNeonatal rat cardiac ventricular myocytesVascular smooth muscle cellsRat cardiac ventricular myocytesSmooth muscle cellsCardiac ventricular myocytesCardiac-specific transcription factor Nkx2.5Receptor expressionResponse elementTranscription factor Nkx2.5Hormone responsivenessVentricular myocytesMuscle cellsSubunit gene promoterModulating activityHormone response elementsCAMP response elementPromoter-luciferase reporter gene constructsGene promoterTransient transfection experimentsRatsFusion geneSubunit geneAP-1 siteDevelopmental regulation of the L-type calcium channel α1C subunit expression in heart
Leu L, O'Hara D, Cala S, Poornima I, Hines R, Marsh J. Developmental regulation of the L-type calcium channel α1C subunit expression in heart. Molecular And Cellular Biochemistry 2000, 205: 101-109. PMID: 10821427, DOI: 10.1023/a:1007013900827.Peer-Reviewed Original ResearchConceptsRNase protection assaysProtection assaysNorthern analysisΑ1C subunitSize of proteinsAlternative splicingAbundance of mRNADevelopmental regulationSmaller transcriptTranscript sizeNative proteinKD proteinMolecular massDevelopmental stagesProteinSubunitsAdult ventriclesTranscriptsPostnatal developmentAbundanceExpressionSubunit expressionCalcium channel propertiesChannel propertiesReceptor message