2005
Discovery of Novel Flavin-Containing Monooxygenase 3 (FMO3) Single Nucleotide Polymorphisms and Functional Analysis of Upstream Haplotype Variants
Koukouritaki S, Poch M, Cabacungan E, McCarver D, Hines R. Discovery of Novel Flavin-Containing Monooxygenase 3 (FMO3) Single Nucleotide Polymorphisms and Functional Analysis of Upstream Haplotype Variants. Molecular Pharmacology 2005, 68: 383-392. PMID: 15858076, DOI: 10.1124/mol.105.012062.Peer-Reviewed Original ResearchConceptsFlavin-containing monooxygenasesFunctional analysisSingle nucleotide polymorphism (SNP) discoveryMembrane interaction domainHaplotype variantsSite-directed mutagenesisConsensus splice sitesFMO3 expressionUpstream SNPsGene functionPolymorphism discoveryNear complete lossRossmann foldFAD bindingInteraction domainSingle nucleotide polymorphismsRegion haplotypesHaplotype 8Exon SNPsFMO enzymesPromoter activityIntron SNPSequence analysisSNP frequenciesLuciferase construct
2003
Characterization of the Human Lung CYP2F1 Gene and Identification of a Novel Lung-specific Binding Motif*
Carr B, Wan J, Hines R, Yost G. Characterization of the Human Lung CYP2F1 Gene and Identification of a Novel Lung-specific Binding Motif*. Journal Of Biological Chemistry 2003, 278: 15473-15483. PMID: 12598524, DOI: 10.1074/jbc.m300319200.Peer-Reviewed Original ResearchConceptsCYP2F1 geneCompetitive electrophoretic mobility shift assaysBacterial artificial chromosome (BAC) clonesElectrophoretic mobility shift assaysDNA-protein complexesE-box motifArtificial chromosome clonesPrimer extension analysisTranscription start siteTissue-specific expressionDNA consensus siteMobility shift assaysTissue-specific regulationUntranslated exon 1Transient transfection studiesLuciferase reporter constructsBox motifChromosome clonesConsensus sitesE-boxNuclear proteinsStart siteExtension analysisCDNA endsUpstream sequencesGenotyping and site-directed mutagenesis of a cytochrome P450 meander Pro-X-Arg motif critical to CYP4B1 catalysis
Zheng Y, Henne K, Charmley P, Kim R, McCarver D, Cabacungan E, Hines R, Rettie A. Genotyping and site-directed mutagenesis of a cytochrome P450 meander Pro-X-Arg motif critical to CYP4B1 catalysis. Toxicology And Applied Pharmacology 2003, 186: 119-126. PMID: 12639503, DOI: 10.1016/s0041-008x(02)00028-5.Peer-Reviewed Original ResearchConceptsHeme bindingArg motifAmino acid sequence alignmentNonhuman primate speciesPrimate speciesRedox partner interactionsSite-directed mutagenesisCYP4B1 geneRestriction enzyme fragment length polymorphismArg triadFragment length polymorphismRabbit CYP4B1Sequence alignmentHuman allelesN-terminalCatalytic functionCommon laboratory animalsAmino acidsPro residuesLength polymorphismMeander regionHuman populationDistal ArgGenesGlu-Arg
1994
A Nomenclature for the Mammalian Flavin-Containing Monooxygenase Gene Family Based on Amino Acid Sequence Identities
Lawton M, Cashman J, Cresteil T, Dolphin C, Elfarra A, Hines R, Hodgson E, Kimura T, Ozols J, Phillips I, Philpot R, Poulsen L, Rettie A, Shephard E, Williams D, Ziegler D. A Nomenclature for the Mammalian Flavin-Containing Monooxygenase Gene Family Based on Amino Acid Sequence Identities. Archives Of Biochemistry And Biophysics 1994, 308: 254-257. PMID: 8311461, DOI: 10.1006/abbi.1994.1035.Peer-Reviewed Original ResearchConceptsAmino acid sequenceGene familyAcid sequenceMammalian FMOsMammalian flavinFMO gene familySingle gene familyGene designationIndividual genesOrthologous formsPercentage identitySpecies linesGenesEnzymeFlavinSequenceFMO4FamilyUnambiguous identificationRelated formsFMO1OrthologsFMO2FMO3Nomenclature