2015
Oxidative stress-responsive transcription factor NRF2 is not indispensable for the human hepatic Flavin-containing monooxygenase-3 (FMO3) gene expression in HepG2 cells
Rudraiah S, Gu X, Hines R, Manautou J. Oxidative stress-responsive transcription factor NRF2 is not indispensable for the human hepatic Flavin-containing monooxygenase-3 (FMO3) gene expression in HepG2 cells. Toxicology In Vitro 2015, 31: 54-59. PMID: 26616280, PMCID: PMC4695222, DOI: 10.1016/j.tiv.2015.11.016.Peer-Reviewed Original ResearchConceptsGene expressionFlavin-containing monooxygenasesStress-responsive transcription factor Nrf2Stress transcription factorsCytosolic regulatory proteinsHepG2 cellsPromoter-luciferase reporter constructsNrf2 target gene expressionGene regulation studiesCo-transfection studiesTarget gene expressionReporter gene activityHeme oxygenase-1Transcription factor Nrf2Luciferase reporter constructsTranscriptional regulationGene regulationKelch-like ECHGene activityTranscription factorsRegulatory proteinsRegulatory pathwaysReporter constructsExpression vectorRegulation studies
2005
MOLECULAR MECHANISMS REGULATING HUMAN CYP4B1 LUNG-SELECTIVE EXPRESSION
Poch M, Cutler N, Yost G, Hines R. MOLECULAR MECHANISMS REGULATING HUMAN CYP4B1 LUNG-SELECTIVE EXPRESSION. Drug Metabolism And Disposition 2005, 33: 1174-1184. PMID: 15900016, DOI: 10.1124/dmd.105.004523.Peer-Reviewed Original ResearchConceptsDistal regulatory elementsRegulatory elementsTransient expressionCompetitive electrophoretic mobility shift assaysDNA/protein binding assaysKrüppel-like factor (KLF) familyElectrophoretic mobility shift assaysTranscription factor recognition sequenceChromatin immunoprecipitation assaysTranscription factor sitesProximal elementMobility shift assaysHepG2 hepatoblastoma cellsProtein binding assaysSp1 bindingSp1 elementImmunoprecipitation assaysNuclear proteinsKidney-derived cellsFactor familyShift assaysFactor sitesMutagenesis studiesRegulatory mechanismsMolecular mechanismsSP1 AND SP3 REGULATE BASAL TRANSCRIPTION OF THE HUMAN CYP2F1 GENE
Wan J, Carr B, Cutler N, Lanza D, Hines R, Yost G. SP1 AND SP3 REGULATE BASAL TRANSCRIPTION OF THE HUMAN CYP2F1 GENE. Drug Metabolism And Disposition 2005, 33: 1244-1253. PMID: 15860659, DOI: 10.1124/dmd.105.004069.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBinding SitesCell Line, TumorCytochrome P-450 Enzyme SystemCytochrome P450 Family 2DNAGene Expression RegulationGenes, ReporterHumansLuciferasesLungMolecular Sequence DataPromoter Regions, GeneticRepressor ProteinsSp1 Transcription FactorSp3 Transcription FactorTranscription, GeneticTranscriptional ActivationTransfectionConceptsSp3 expression vectorsExpression vectorCYP2F1 geneDrosophila Schneider line 2 cellsSchneider line 2 cellsSpecific protein-DNA complexesSp1 expression vectorSL-2 cellsProtein-DNA complexesProtein-DNA bindingTATA-less promoter regionA549 cellsCell nuclear extractsCompetitive electrophoretic mobilityBasal transcription elementProximal promoter elementsLuciferase reporter constructsSp1-like sitesSp3 factorsSp3 proteinsSelective transcriptionSupershift studiesGene transcriptionPromoter elementsTranscription elements
2003
Characterization of the Human Lung CYP2F1 Gene and Identification of a Novel Lung-specific Binding Motif*
Carr B, Wan J, Hines R, Yost G. Characterization of the Human Lung CYP2F1 Gene and Identification of a Novel Lung-specific Binding Motif*. Journal Of Biological Chemistry 2003, 278: 15473-15483. PMID: 12598524, DOI: 10.1074/jbc.m300319200.Peer-Reviewed Original ResearchConceptsCYP2F1 geneCompetitive electrophoretic mobility shift assaysBacterial artificial chromosome (BAC) clonesElectrophoretic mobility shift assaysDNA-protein complexesE-box motifArtificial chromosome clonesPrimer extension analysisTranscription start siteTissue-specific expressionDNA consensus siteMobility shift assaysTissue-specific regulationUntranslated exon 1Transient transfection studiesLuciferase reporter constructsBox motifChromosome clonesConsensus sitesE-boxNuclear proteinsStart siteExtension analysisCDNA endsUpstream sequences
1999
ROLE OF CANONICAL GLUCOCORTICOID RESPONSIVE ELEMENTS IN MODULATING EXPRESSION OF GENES REGULATED BY THE ARYLHYDROCARBON RECEPTOR*
LINDER M, FALKNER K, SRINIVASAN G, HINES R, PROUGH R. ROLE OF CANONICAL GLUCOCORTICOID RESPONSIVE ELEMENTS IN MODULATING EXPRESSION OF GENES REGULATED BY THE ARYLHYDROCARBON RECEPTOR*. Drug Metabolism Reviews 1999, 31: 247-271. PMID: 10065375, DOI: 10.1081/dmr-100101917.Peer-Reviewed Original Research
1989
Glucocorticoid regulation of the rat cytochrome P450c (P450IA1) gene: Receptor binding within intron I
Mathis J, Houser W, Bresnick E, Cidlowski J, Hines R, Prough R, Simpson E. Glucocorticoid regulation of the rat cytochrome P450c (P450IA1) gene: Receptor binding within intron I. Archives Of Biochemistry And Biophysics 1989, 269: 93-105. PMID: 2916850, DOI: 10.1016/0003-9861(89)90090-8.Peer-Reviewed Original ResearchConceptsGlucocorticoid receptorConcentrations of dexamethasoneUntreated cellsMRNA contentFetal rat hepatocytesHalf-maximal increaseAddition of dexamethasonePresence of dexamethasoneCultured fetal rat hepatocytesGlucocorticoid-responsive elementRat hepatocytesDexamethasoneCytochrome P450cGlucocorticoid regulationHepG2 hepatoma cellsGlucocorticoidsAcetyltransferase activityHepatic cytochromeChloramphenicol acetyltransferase activityReceptorsNorthern blot analysisBlot analysisHepatoma cellsReceptor fraction
1988
Identification of multiple regulatory elements on the human cytochrome P450IA1 gene
Hines R, Mathis J, Jacob C. Identification of multiple regulatory elements on the human cytochrome P450IA1 gene. Carcinogenesis 1988, 9: 1599-1605. PMID: 3409463, DOI: 10.1093/carcin/9.9.1599.Peer-Reviewed Original ResearchInduction of cytochrome P-450IA1 gene expression in human breast tumour cell lines
Pasanen M, Stacey S, Lykkesfeldt A, Briand P, Hines R, Autrup H. Induction of cytochrome P-450IA1 gene expression in human breast tumour cell lines. Chemico-Biological Interactions 1988, 66: 223-232. PMID: 2456161, DOI: 10.1016/0009-2797(88)90073-7.Peer-Reviewed Original ResearchConceptsBreast tumor cell linesHuman breast tumor cell linesTumor cell linesTCDD treatmentCell linesDifferent breast tumor cell linesConcentrations of TCDDZR-75BT-20Cytochrome P-450IA1NM TCDDCAMA-1Basal expression levelsControl cellsExpression levelsMCF-7Northern blot analysisBlot analysisTCDDTreatmentTotal RNAP-450IA1TCDD inductionDiol metabolitesInduction
1985
3-Methylcholanthrene-induced expression of the cytochrome P-450c gene
Foldes R, Hines R, Ho K, Shen M, Nagel K, Bresnick E. 3-Methylcholanthrene-induced expression of the cytochrome P-450c gene. Archives Of Biochemistry And Biophysics 1985, 239: 137-146. PMID: 4004253, DOI: 10.1016/0003-9861(85)90820-3.Peer-Reviewed Original ResearchConceptsNuclear RNA speciesCytochrome P-450c mRNARNA speciesCytochrome P-450c geneP-450c geneClone 46Nuclear transcription systemNuclear RNA precursorsNuclear RNA transportRat serum albumin geneDot hybridization experimentsSerum albumin geneNorthern blot analysisTranscriptional controlRNA transportRNA precursorsNuclear RNARegulation of inductionHybridization experimentsTranscription systemM13 subclonesAlbumin geneCytochrome P-450cRNA inductionSpecies
1983
Molecular induction by phenobarbital of a rat hepatic form of cytochrome P-450: Expression of a 4-kilobase messenger RNA
Omiecinski C, Hines R, Foldes R, Levy J, Bresnick E. Molecular induction by phenobarbital of a rat hepatic form of cytochrome P-450: Expression of a 4-kilobase messenger RNA. Archives Of Biochemistry And Biophysics 1983, 227: 478-493. PMID: 6141764, DOI: 10.1016/0003-9861(83)90478-2.Peer-Reviewed Original Research