2004
Two distinct classes of CCAAT box elements that bind nuclear factor-Y/α-actinin-4: potential role in human CYP1A1 regulation
Poch M, Al-Kassim L, Smolinski S, Hines R. Two distinct classes of CCAAT box elements that bind nuclear factor-Y/α-actinin-4: potential role in human CYP1A1 regulation. Toxicology And Applied Pharmacology 2004, 199: 239-250. PMID: 15364540, DOI: 10.1016/j.taap.2003.12.023.Peer-Reviewed Original ResearchMeSH KeywordsActininBlotting, SouthwesternCCAAT-Binding FactorChromatinCytochrome P-450 CYP1A1Electrophoresis, Polyacrylamide GelElectrophoretic Mobility Shift AssayEnzyme InhibitorsGene Expression Regulation, EnzymologicHeLa CellsHumansPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPrecipitin TestsProtein BindingTranscription Factors
2003
Trichloroethylene Decreases Heat Shock Protein 90 Interactions with Endothelial Nitric Oxide Synthase: Implications for Endothelial Cell Proliferation
Ou J, Ou Z, McCarver D, Hines R, Oldham K, Ackerman A, Pritchard K. Trichloroethylene Decreases Heat Shock Protein 90 Interactions with Endothelial Nitric Oxide Synthase: Implications for Endothelial Cell Proliferation. Toxicological Sciences 2003, 73: 90-97. PMID: 12657742, DOI: 10.1093/toxsci/kfg062.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCalcimycinCattleCell DivisionCells, CulturedEndothelial CellsEnzyme InhibitorsHSP90 Heat-Shock ProteinsIonophoresNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IIIPhosphorylationPrecipitin TestsSuperoxidesTrichloroethyleneVascular Endothelial Growth Factor AConceptsEndothelial cell proliferationCell proliferationNormal blood vessel growthHeat shock protein 90Shock protein 90Serine 1179Hsp90 interactionEndothelial cellsProtein 90Blood vessel growthENOS functionImportant roleProteinActivation stateInhibited VEGFVessel growthGrowth factorBalance of NONew insightsProliferationVascular endothelial growth factorNitric oxideEndothelial growth factorEndothelial nitric oxide synthaseEnvironmental pollutantsGenetics and Susceptibility to Toxic Chemicals: Do You (or Should You) Know Your Genetic Profile?
Lash L, Hines R, Gonzalez F, Zacharewski T, Rothstein M. Genetics and Susceptibility to Toxic Chemicals: Do You (or Should You) Know Your Genetic Profile? Journal Of Pharmacology And Experimental Therapeutics 2003, 305: 403-409. PMID: 12606672, DOI: 10.1124/jpet.102.039925.Peer-Reviewed Original ResearchConceptsSpecific enzyme systemsExperimental Biology meetingTransgenic technologyGenetic determinantsEnzyme systemGenetic profileToxic chemicalsGeneticsDivision of ToxicologyComplex interplayHuman healthIndividual susceptibilityPharmacogenomicsExperimental therapeuticsPhysiologySusceptibilityChemicalsDivisionTherapeutics
2000
Diflubenzuron, a Benzoyl-Urea Insecticide, Is a Potent Inhibitor of TCDD-Induced CYP1A1 Expression in HepG2 Cells
Ledirac N, Delescluse C, Lesca P, Piechocki M, Hines R, de Sousa G, Pralavorio M, Rahmani R. Diflubenzuron, a Benzoyl-Urea Insecticide, Is a Potent Inhibitor of TCDD-Induced CYP1A1 Expression in HepG2 Cells. Toxicology And Applied Pharmacology 2000, 164: 273-279. PMID: 10799337, DOI: 10.1006/taap.2000.8920.Peer-Reviewed Original ResearchConceptsCYP1A1 expressionEthoxyresorufin-O-deethylase (EROD) activityHepG2 cellsStrong dose-dependent decreaseDose-dependent decreaseB16 murine melanomaHuman hepatoma cells HepG2Receptor antagonismTreatment of HepG2Hepatoma cells HepG2Human CYP1A1 promoterParental HepG2 cellsCYP1A1 inducerNoncytotoxic concentrationsMurine melanomaChloramphenicol acetyltransferase (CAT) reporter geneAdditional studiesCYP1A1 inductionNorthern blot analysisCell linesBlot analysisSynthesis inhibitorH exposurePotent inhibitorAh receptor