2014
Tolerance to Acetaminophen Hepatotoxicity in the Mouse Model of Autoprotection Is Associated with Induction of Flavin-Containing Monooxygenase-3 (FMO3) in Hepatocytes
Rudraiah S, Rohrer P, Gurevich I, Goedken M, Rasmussen T, Hines R, Manautou J. Tolerance to Acetaminophen Hepatotoxicity in the Mouse Model of Autoprotection Is Associated with Induction of Flavin-Containing Monooxygenase-3 (FMO3) in Hepatocytes. Toxicological Sciences 2014, 141: 263-277. PMID: 24973094, PMCID: PMC4271123, DOI: 10.1093/toxsci/kfu124.Peer-Reviewed Original ResearchConceptsMale C57BL/6J miceAPAP hepatotoxicityFlavin-Containing Monooxygenase 3Protein expressionC57BL/6J miceFemale miceMouse modelMRNA levelsFirst time inductionNovel protective functionSingle doseAPAP treatmentMale miceAPAP cytotoxicityAcetaminophen pretreatmentHepatotoxic doseHigh doseAcetaminophen hepatotoxicityHepatotoxicityMice resultsCell line clonesMRNA expressionFMO3 expressionMiceAPAP
2001
Induction of cytochrome P450 1A1 gene expression, oxidative stress, and genotoxicity by carbaryl and thiabendazole in transfected human HepG2 and lymphoblastoid cells
Delescluse C, Ledirac N, Li R, Piechocki M, Hines R, Gidrol X, Rahmani R. Induction of cytochrome P450 1A1 gene expression, oxidative stress, and genotoxicity by carbaryl and thiabendazole in transfected human HepG2 and lymphoblastoid cells. Biochemical Pharmacology 2001, 61: 399-407. PMID: 11226373, DOI: 10.1016/s0006-2952(00)00562-1.Peer-Reviewed Original ResearchMeSH KeywordsCarbarylChloramphenicol O-AcetyltransferaseCholinesterase InhibitorsCytochrome P-450 CYP1A1DNA DamageEndoplasmic Reticulum Chaperone BiPEnzyme InductionGene ExpressionHeat-Shock ProteinsHumansMutagenicity TestsOxidative StressThiabendazoleTranscription, GeneticTransfectionTumor Cells, CulturedConceptsXenobiotic responsive elementStress genesChloramphenicol acetyl transferase (CAT) reporter geneAcetyl transferase reporter geneLevel of transcriptionTransferase reporter geneOxidative stressCell linesHuman hepatoma HepG2 cell lineHuman lymphoblastoid cell linesTranscriptional regulationHepatoma HepG2 cell lineCytochrome P450 1A1 gene expressionReporter CATTranscriptional levelReporter geneXRE sequenceLymphoblastoid cell linesGene expressionFusion constructsIntracellular signalsParental linesCYP1A1 cDNAResponse elementResponsive element
1998
A Genetic Polymorphism in the Regulatory Sequences of HumanCYP2E1:Association with Increased Chlorzoxazone Hydroxylation in the Presence of Obesity and Ethanol Intake
McCarver D, Byun R, Hines R, Hichme M, Wegenek W. A Genetic Polymorphism in the Regulatory Sequences of HumanCYP2E1:Association with Increased Chlorzoxazone Hydroxylation in the Presence of Obesity and Ethanol Intake. Toxicology And Applied Pharmacology 1998, 152: 276-281. PMID: 9772223, DOI: 10.1006/taap.1998.8532.Peer-Reviewed Original ResearchConceptsMetabolic abilitiesDNA samplesHuman DNA samplesDNA insertionRegulatory sequencesRegulatory regionsXbaI fragmentGenomic probesRegulatory polymorphismsRestriction enzyme XbaIRestriction enzyme digestionSame DNA samplesInsertion mutationsUpstream regionRestriction enzymesSouthern blotFunctional significanceSouthern blottingMutationsEnzyme XbaIEnzyme digestionGenetic polymorphismsVivo CYP2E1 activityFunctional characterization of the human CYP1A1 negative regulatory element: modulation of Ah receptor mediated transcriptional activity.
Piechocki M, Hines R. Functional characterization of the human CYP1A1 negative regulatory element: modulation of Ah receptor mediated transcriptional activity. Carcinogenesis 1998, 19: 771-780. PMID: 9635862, DOI: 10.1093/carcin/19.5.771.Peer-Reviewed Original Research
1997
Variation in Induction of Human Placental CYP2E1: Possible Role in Susceptibility to Fetal Alcohol Syndrome?
Rasheed A, Hines R, McCarver-May D. Variation in Induction of Human Placental CYP2E1: Possible Role in Susceptibility to Fetal Alcohol Syndrome? Toxicology And Applied Pharmacology 1997, 144: 396-400. PMID: 9194424, DOI: 10.1006/taap.1997.8152.Peer-Reviewed Original ResearchConceptsFetal alcohol syndromeAlcohol syndromeIntersubject variationHeavy drinking womenSmall birth weightAbsolute alcohol intakeWestern blot analysisBirth lengthAlcohol intakeDrinking womenHepatic CYP2E1Birth weightTerm placentaHeavy drinkersAlcohol metabolismCYP2E1Placental microsomesHuman placentaHeavy drinkingPlacentaTime pointsPotential mechanismsEnvironmental agentsWomenPregnancy
1995
Developmental regulation of flavin-containing monooxygenase (FMO) isoforms 1 and 2 in pregnant rabbit
Lee M, Smiley S, Kadkhodayan S, Hines R, Williams D. Developmental regulation of flavin-containing monooxygenase (FMO) isoforms 1 and 2 in pregnant rabbit. Chemico-Biological Interactions 1995, 96: 75-85. PMID: 7720106, DOI: 10.1016/0009-2797(94)03584-u.Peer-Reviewed Original ResearchConceptsCortisol levelsRabbit lungsClassical cytochrome P450 inducersPeaks of progesteroneCytochrome P450 inducersPlasma cortisol levelsMaternal kidneyPregnant rabbitsPlasma progesteroneP450 inducersProgesteroneLungMRNA levelsHormonal regulationGestationMacrolide antibioticsTime courseKidneyFMO activityLiverAdministrationFMO2Mammalian flavinDiverse xenobioticsNumber of laboratories
1994
Restoration of aryl hydrocarbon (Ah) responsiveness in MDA-MB-231 human breast cancer cells by transient expression of the estrogen receptor
Thomsen J, Wang X, Hines R, Safe S. Restoration of aryl hydrocarbon (Ah) responsiveness in MDA-MB-231 human breast cancer cells by transient expression of the estrogen receptor. Carcinogenesis 1994, 15: 933-937. PMID: 8200098, DOI: 10.1093/carcin/15.5.933.Peer-Reviewed Original ResearchConceptsMDA-MB-231 cellsHuman breast cancer cellsMDA-MB-231 human breast cancer cellsAh-responsivenessBreast cancer cellsNM TCDDHuman estrogen receptor expression plasmidAryl hydrocarbon responsivenessCancer cellsReceptor expression plasmidAryl hydrocarbon receptorProgesterone receptorEstrogen receptorResponsive plasmidCYP1A1 gene expressionHydrocarbon receptorReceptorsCell linesCYP1A1 geneCAT activityExpression of nuclearParallel studyHuman CYP1A1 geneCellsExpression plasmid
1993
Expression of human cytochrome P450 1A1 in DNA repair deficient and proficient human fibroblasts stably transformed with an inducible expression vector
States J, Quan T, Hines R, Novak R, Runge-Morris M. Expression of human cytochrome P450 1A1 in DNA repair deficient and proficient human fibroblasts stably transformed with an inducible expression vector. Carcinogenesis 1993, 14: 1643-1649. PMID: 8353849, DOI: 10.1093/carcin/14.8.1643.Peer-Reviewed Original ResearchMeSH Keywords7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxideAnimalsBase SequenceBenzo(a)pyreneBenzoflavonesBiotransformationCadmiumCadmium CompoundsChimeraCytochrome P-450 Enzyme SystemDNA RepairEnzyme InductionFibroblastsGene Expression Regulation, EnzymologicGenetic VectorsHumansIsoenzymesMiceMolecular Sequence DataPlasmidsRNA, MessengerSulfatesTransfectionTransformation, GeneticXeroderma PigmentosumConceptsCytochrome P450 1A1P450 1A1Inducible CYP1A1Inducible mouse metallothionein-I promoterLow dosesHuman skin fibroblastsProficient cellsPotent mutagenic agentCYP1A1 expressionCYP1A1 activityHuman cytochrome P450 1A1DNA repairCYP1A1Mouse metallothionein-I promoterCytotoxic effectsMetallothionein-I promoterSkin fibroblastsDifferential cytotoxicityDNA repair-proficient cellsToxic effectsMore cytotoxicCytochrome P450Normal cellsRepairHuman CYP1A1
1991
Differences in 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin‐inducible CYP1A1 expression in human breast carcinoma cell lines involve altered trans‐acting factors
THOMSEN J, NISSEN L, STACEY S, HINES R, AUTRUP H. Differences in 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin‐inducible CYP1A1 expression in human breast carcinoma cell lines involve altered trans‐acting factors. The FEBS Journal 1991, 197: 577-582. PMID: 2029891, DOI: 10.1111/j.1432-1033.1991.tb15946.x.Peer-Reviewed Original Research
1990
Immunochemical and molecular biological studies on human placental cigarette smoke-inducible cytochrome P-450-dependent monooxygenase activities
Pasanen M, Haaparanta T, Sundin M, Sivonen P, Vakakangas K, Raunio H, Hines R, Gustafsson J, Pelkonen O. Immunochemical and molecular biological studies on human placental cigarette smoke-inducible cytochrome P-450-dependent monooxygenase activities. Toxicology 1990, 62: 175-187. PMID: 1693794, DOI: 10.1016/0300-483x(90)90108-s.Peer-Reviewed Original ResearchMeSH Keywords7-Alkoxycoumarin O-DealkylaseAryl Hydrocarbon HydroxylasesBlotting, NorthernCytochrome P-450 CYP1A1Cytochrome P-450 Enzyme InhibitorsCytochrome P-450 Enzyme SystemEnzyme InductionFemaleGene Expression Regulation, EnzymologicHumansImmunoblottingImmunodiffusionMicrosomesNADPH-Ferrihemoprotein ReductaseOxidoreductasesOxygenasesPlacentaPregnancyRNASmokingConceptsMaternal cigarette smokingAryl hydrocarbon hydroxylaseHuman placental microsomesSmoking mothersCigarette smokingPlacental microsomesHuman placentaMonoclonal antibodiesO-deethylase activityMaternal smokingMonooxygenase activitySmoking statusSignificant inhibitory effectPlacental tissueMAb 2ERDE activitySmokingMAb 1Inhibitory effectLiver NADPH-cytochrome PO-deethylaseECDE activitiesPlacentaCYPIA1 geneAntibodies
1987
Aflatoxin B1-4-hydroxylase is associated with cytochrome P3-450 in C57BL6 mouse liver
Koser P, Faletto M, Bansal S, Caballes L, Bresnick E, Hines R, Gurtoo H. Aflatoxin B1-4-hydroxylase is associated with cytochrome P3-450 in C57BL6 mouse liver. Biochemical And Biophysical Research Communications 1987, 142: 872-878. PMID: 3103616, DOI: 10.1016/0006-291x(87)91494-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAroclorsAryl Hydrocarbon HydroxylasesBenzoflavonesBeta-NaphthoflavoneChlorodiphenyl (54% Chlorine)Cytochrome P-450 Enzyme SystemDNAEnzyme InductionFemaleLiverMethylcholanthreneMiceMice, Inbred C57BLMixed Function OxygenasesNucleic Acid HybridizationPolychlorinated BiphenylsRNA, Messenger
1986
Regulation of cytochrome P-450c by glucocorticoids and polycyclic aromatic hydrocarbons in cultured fetal rat hepatocytes
Mathis J, Prough R, Hines R, Bresnick E, Simpson E. Regulation of cytochrome P-450c by glucocorticoids and polycyclic aromatic hydrocarbons in cultured fetal rat hepatocytes. Archives Of Biochemistry And Biophysics 1986, 246: 439-448. PMID: 2870681, DOI: 10.1016/0003-9861(86)90490-x.Peer-Reviewed Original ResearchThe molecular biology of the polycyclic aromatic hydrocarbon inducible cytochrome P‐450; the past is prologue
Iversen P, Hines R, Bresnick E. The molecular biology of the polycyclic aromatic hydrocarbon inducible cytochrome P‐450; the past is prologue. BioEssays 1986, 4: 15-19. PMID: 3790107, DOI: 10.1002/bies.950040105.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCytochrome P-450 Enzyme SystemEnzyme InductionGenesMicrosomes, LiverPolycyclic CompoundsRatsConceptsRegulation of expressionEnzyme systemUbiquitous familySubstrate specificityGene expressionMultiple isozymesMolecular biologyCytochrome PToxic lesionsExogenous compoundsOxidative metabolismMechanisms of metabolismExpressionInducible cytochrome PIsozymesComplete understandingMetabolismBiochemical pharmacologyOrganismsBiologySpeciesRegulationWide varietyCascadeCurrent notions
1985
3-Methylcholanthrene-induced expression of the cytochrome P-450c gene
Foldes R, Hines R, Ho K, Shen M, Nagel K, Bresnick E. 3-Methylcholanthrene-induced expression of the cytochrome P-450c gene. Archives Of Biochemistry And Biophysics 1985, 239: 137-146. PMID: 4004253, DOI: 10.1016/0003-9861(85)90820-3.Peer-Reviewed Original ResearchConceptsNuclear RNA speciesCytochrome P-450c mRNARNA speciesCytochrome P-450c geneP-450c geneClone 46Nuclear transcription systemNuclear RNA precursorsNuclear RNA transportRat serum albumin geneDot hybridization experimentsSerum albumin geneNorthern blot analysisTranscriptional controlRNA transportRNA precursorsNuclear RNARegulation of inductionHybridization experimentsTranscription systemM13 subclonesAlbumin geneCytochrome P-450cRNA inductionSpecies
1984
Induction of cytochrome P450 by xenobiotics.
Bresnick E, Foldes R, Hines R. Induction of cytochrome P450 by xenobiotics. Pharmacological Reviews 1984, 36: 43s-51s. PMID: 6433366.Peer-Reviewed Original Research
1983
Molecular induction by phenobarbital of a rat hepatic form of cytochrome P-450: Expression of a 4-kilobase messenger RNA
Omiecinski C, Hines R, Foldes R, Levy J, Bresnick E. Molecular induction by phenobarbital of a rat hepatic form of cytochrome P-450: Expression of a 4-kilobase messenger RNA. Archives Of Biochemistry And Biophysics 1983, 227: 478-493. PMID: 6141764, DOI: 10.1016/0003-9861(83)90478-2.Peer-Reviewed Original Research