2016
Role of Chromatin Structural Changes in Regulating Human CYP3A Ontogeny
Giebel N, Shadley J, McCarver D, Dorko K, Gramignoli R, Strom S, Yan K, Simpson P, Hines R. Role of Chromatin Structural Changes in Regulating Human CYP3A Ontogeny. Drug Metabolism And Disposition 2016, 44: 1027-1037. PMID: 26921389, PMCID: PMC4931893, DOI: 10.1124/dmd.116.069344.Peer-Reviewed Original ResearchMeSH KeywordsAdultAge FactorsAgedAged, 80 and overBinding SitesChildChild, PreschoolChromatinChromatin Assembly and DisassemblyCytochrome P-450 CYP3AGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicGestational AgeHepatocytesHistonesHumansInfantLiverMiddle AgedNucleic Acid ConformationPromoter Regions, GeneticProtein ConformationStructure-Activity RelationshipTranscription, Genetic
2007
Identification and Functional Analysis of a Novel Human CYP2E1 Far Upstream Enhancer
Shadley J, Divakaran K, Munson K, Hines R, Douglas K, McCarver D. Identification and Functional Analysis of a Novel Human CYP2E1 Far Upstream Enhancer. Molecular Pharmacology 2007, 71: 1630-1639. PMID: 17353354, DOI: 10.1124/mol.106.031302.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBinding SitesCells, CulturedCytochrome P-450 CYP2E1Enhancer Elements, GeneticGATA4 Transcription FactorGene Expression Regulation, EnzymologicGenetic VariationHepatocytesHumansMolecular Sequence DataPromoter Regions, GeneticRepetitive Sequences, Nucleic AcidSteroidogenic Factor 1Trans-ActivatorsTranscriptional ActivationConceptsElectrophoretic mobility shift assaysEnhancer sequencesCompetitive electrophoretic mobility shift assaysSupershift electrophoretic mobility shift assaysFunctional regulatory elementsGATA family membersMobility shift assaysGreater luciferase activityOrphan nuclear receptorSteroidogenic factor 1Luciferase reporter activityGATA sequencesFetoprotein transcription factorGATA familyChromatin immunoprecipitationTranscription factorsNuclear proteinsRegulatory elementsShift assaysRegulatory mechanismsFunctional analysisPromoter constructsDirect repeatsReporter activityUpstream region
2005
MOLECULAR MECHANISMS REGULATING HUMAN CYP4B1 LUNG-SELECTIVE EXPRESSION
Poch M, Cutler N, Yost G, Hines R. MOLECULAR MECHANISMS REGULATING HUMAN CYP4B1 LUNG-SELECTIVE EXPRESSION. Drug Metabolism And Disposition 2005, 33: 1174-1184. PMID: 15900016, DOI: 10.1124/dmd.105.004523.Peer-Reviewed Original ResearchConceptsDistal regulatory elementsRegulatory elementsTransient expressionCompetitive electrophoretic mobility shift assaysDNA/protein binding assaysKrüppel-like factor (KLF) familyElectrophoretic mobility shift assaysTranscription factor recognition sequenceChromatin immunoprecipitation assaysTranscription factor sitesProximal elementMobility shift assaysHepG2 hepatoblastoma cellsProtein binding assaysSp1 bindingSp1 elementImmunoprecipitation assaysNuclear proteinsKidney-derived cellsFactor familyShift assaysFactor sitesMutagenesis studiesRegulatory mechanismsMolecular mechanismsSP1 AND SP3 REGULATE BASAL TRANSCRIPTION OF THE HUMAN CYP2F1 GENE
Wan J, Carr B, Cutler N, Lanza D, Hines R, Yost G. SP1 AND SP3 REGULATE BASAL TRANSCRIPTION OF THE HUMAN CYP2F1 GENE. Drug Metabolism And Disposition 2005, 33: 1244-1253. PMID: 15860659, DOI: 10.1124/dmd.105.004069.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBinding SitesCell Line, TumorCytochrome P-450 Enzyme SystemCytochrome P450 Family 2DNAGene Expression RegulationGenes, ReporterHumansLuciferasesLungMolecular Sequence DataPromoter Regions, GeneticRepressor ProteinsSp1 Transcription FactorSp3 Transcription FactorTranscription, GeneticTranscriptional ActivationTransfectionConceptsSp3 expression vectorsExpression vectorCYP2F1 geneDrosophila Schneider line 2 cellsSchneider line 2 cellsSpecific protein-DNA complexesSp1 expression vectorSL-2 cellsProtein-DNA complexesProtein-DNA bindingTATA-less promoter regionA549 cellsCell nuclear extractsCompetitive electrophoretic mobilityBasal transcription elementProximal promoter elementsLuciferase reporter constructsSp1-like sitesSp3 factorsSp3 proteinsSelective transcriptionSupershift studiesGene transcriptionPromoter elementsTranscription elements
2003
Characterization of the Human Lung CYP2F1 Gene and Identification of a Novel Lung-specific Binding Motif*
Carr B, Wan J, Hines R, Yost G. Characterization of the Human Lung CYP2F1 Gene and Identification of a Novel Lung-specific Binding Motif*. Journal Of Biological Chemistry 2003, 278: 15473-15483. PMID: 12598524, DOI: 10.1074/jbc.m300319200.Peer-Reviewed Original ResearchConceptsCYP2F1 geneCompetitive electrophoretic mobility shift assaysBacterial artificial chromosome (BAC) clonesElectrophoretic mobility shift assaysDNA-protein complexesE-box motifArtificial chromosome clonesPrimer extension analysisTranscription start siteTissue-specific expressionDNA consensus siteMobility shift assaysTissue-specific regulationUntranslated exon 1Transient transfection studiesLuciferase reporter constructsBox motifChromosome clonesConsensus sitesE-boxNuclear proteinsStart siteExtension analysisCDNA endsUpstream sequences
1996
Identification of Multiple Rabbit Flavin-Containing Monooxygenase Form 1 (FMO1) Gene Promoters and Observation of Tissue-Specific DNase I Hypersensitive Sites
Luo Z, Hines R. Identification of Multiple Rabbit Flavin-Containing Monooxygenase Form 1 (FMO1) Gene Promoters and Observation of Tissue-Specific DNase I Hypersensitive Sites. Archives Of Biochemistry And Biophysics 1996, 336: 251-260. PMID: 8954572, DOI: 10.1006/abbi.1996.0555.Peer-Reviewed Original Research
1995
Partial Characterization of the Human CYP1A1 Negatively Acting Transcription Factor and Mutational Analysis of Its Cognate DNA Recognition Sequence
Boucher P, Piechocki M, Hines R. Partial Characterization of the Human CYP1A1 Negatively Acting Transcription Factor and Mutational Analysis of Its Cognate DNA Recognition Sequence. Molecular And Cellular Biology 1995, 15: 5144-5151. PMID: 7651431, PMCID: PMC230761, DOI: 10.1128/mcb.15.9.5144.Peer-Reviewed Original ResearchBase SequenceBinding SitesBinding, CompetitiveCells, CulturedCytochrome P-450 Enzyme SystemDNA Mutational AnalysisGene Expression Regulation, EnzymologicHumansMolecular Sequence DataNuclear ProteinsOxygenasesProtein BindingRegulatory Sequences, Nucleic AcidSequence Homology, Nucleic AcidTranscription Factors