1990
Immunochemical and molecular biological studies on human placental cigarette smoke-inducible cytochrome P-450-dependent monooxygenase activities
Pasanen M, Haaparanta T, Sundin M, Sivonen P, Vakakangas K, Raunio H, Hines R, Gustafsson J, Pelkonen O. Immunochemical and molecular biological studies on human placental cigarette smoke-inducible cytochrome P-450-dependent monooxygenase activities. Toxicology 1990, 62: 175-187. PMID: 1693794, DOI: 10.1016/0300-483x(90)90108-s.Peer-Reviewed Original ResearchMeSH Keywords7-Alkoxycoumarin O-DealkylaseAryl Hydrocarbon HydroxylasesBlotting, NorthernCytochrome P-450 CYP1A1Cytochrome P-450 Enzyme InhibitorsCytochrome P-450 Enzyme SystemEnzyme InductionFemaleGene Expression Regulation, EnzymologicHumansImmunoblottingImmunodiffusionMicrosomesNADPH-Ferrihemoprotein ReductaseOxidoreductasesOxygenasesPlacentaPregnancyRNASmokingConceptsMaternal cigarette smokingAryl hydrocarbon hydroxylaseHuman placental microsomesSmoking mothersCigarette smokingPlacental microsomesHuman placentaMonoclonal antibodiesO-deethylase activityMaternal smokingMonooxygenase activitySmoking statusSignificant inhibitory effectPlacental tissueMAb 2ERDE activitySmokingMAb 1Inhibitory effectLiver NADPH-cytochrome PO-deethylaseECDE activitiesPlacentaCYPIA1 geneAntibodies
1988
Induction of cytochrome P-450IA1 gene expression in human breast tumour cell lines
Pasanen M, Stacey S, Lykkesfeldt A, Briand P, Hines R, Autrup H. Induction of cytochrome P-450IA1 gene expression in human breast tumour cell lines. Chemico-Biological Interactions 1988, 66: 223-232. PMID: 2456161, DOI: 10.1016/0009-2797(88)90073-7.Peer-Reviewed Original ResearchConceptsBreast tumor cell linesHuman breast tumor cell linesTumor cell linesTCDD treatmentCell linesDifferent breast tumor cell linesConcentrations of TCDDZR-75BT-20Cytochrome P-450IA1NM TCDDCAMA-1Basal expression levelsControl cellsExpression levelsMCF-7Northern blot analysisBlot analysisTCDDTreatmentTotal RNAP-450IA1TCDD inductionDiol metabolitesInduction