2015
Oxidative stress-responsive transcription factor NRF2 is not indispensable for the human hepatic Flavin-containing monooxygenase-3 (FMO3) gene expression in HepG2 cells
Rudraiah S, Gu X, Hines R, Manautou J. Oxidative stress-responsive transcription factor NRF2 is not indispensable for the human hepatic Flavin-containing monooxygenase-3 (FMO3) gene expression in HepG2 cells. Toxicology In Vitro 2015, 31: 54-59. PMID: 26616280, PMCID: PMC4695222, DOI: 10.1016/j.tiv.2015.11.016.Peer-Reviewed Original ResearchConceptsGene expressionFlavin-containing monooxygenasesStress-responsive transcription factor Nrf2Stress transcription factorsCytosolic regulatory proteinsHepG2 cellsPromoter-luciferase reporter constructsNrf2 target gene expressionGene regulation studiesCo-transfection studiesTarget gene expressionReporter gene activityHeme oxygenase-1Transcription factor Nrf2Luciferase reporter constructsTranscriptional regulationGene regulationKelch-like ECHGene activityTranscription factorsRegulatory proteinsRegulatory pathwaysReporter constructsExpression vectorRegulation studies
2008
CYP3A4 regulation by DBP and E4BP4 transcription factors
Maas K, Le M, Hines R. CYP3A4 regulation by DBP and E4BP4 transcription factors. The FASEB Journal 2008, 22: 921.7-921.7. DOI: 10.1096/fasebj.22.1_supplement.921.7.Peer-Reviewed Original ResearchD-element binding proteinDose-dependent mannerCYP3A4 promoter activityPromoter activityLuciferase expressionCYP3A4 regulationPostnatal liverLuciferase reporterProtein 4Luciferase activityHepG2 cellsE4BP4Binding proteinDevelopmental expression patternsResponsive elementExpression patternsHigh affinityProximal promoterTranscription factorsExpressionActivity
2007
Identification and Functional Analysis of a Novel Human CYP2E1 Far Upstream Enhancer
Shadley J, Divakaran K, Munson K, Hines R, Douglas K, McCarver D. Identification and Functional Analysis of a Novel Human CYP2E1 Far Upstream Enhancer. Molecular Pharmacology 2007, 71: 1630-1639. PMID: 17353354, DOI: 10.1124/mol.106.031302.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBinding SitesCells, CulturedCytochrome P-450 CYP2E1Enhancer Elements, GeneticGATA4 Transcription FactorGene Expression Regulation, EnzymologicGenetic VariationHepatocytesHumansMolecular Sequence DataPromoter Regions, GeneticRepetitive Sequences, Nucleic AcidSteroidogenic Factor 1Trans-ActivatorsTranscriptional ActivationConceptsElectrophoretic mobility shift assaysEnhancer sequencesCompetitive electrophoretic mobility shift assaysSupershift electrophoretic mobility shift assaysFunctional regulatory elementsGATA family membersMobility shift assaysGreater luciferase activityOrphan nuclear receptorSteroidogenic factor 1Luciferase reporter activityGATA sequencesFetoprotein transcription factorGATA familyChromatin immunoprecipitationTranscription factorsNuclear proteinsRegulatory elementsShift assaysRegulatory mechanismsFunctional analysisPromoter constructsDirect repeatsReporter activityUpstream regionRegulation of CYP3A4 Expression by PAR Transcription Factors
Mantei K, Le M, Klick D, Vyhlidal C, Hines R. Regulation of CYP3A4 Expression by PAR Transcription Factors. The FASEB Journal 2007, 21: a1183-a1183. DOI: 10.1096/fasebj.21.6.a1183-a.Peer-Reviewed Original ResearchD-element binding proteinCYP3A4 expressionHepatic leukemia factorCYP3A4 promoter activityAdult human liverAbundant cytochrome P450CYP3A4 promoterHuman liverPostnatal liverProtein 4DBP expressionPAR familyEmbryonic factorsHepG2 cellsTranscription factorsCytochrome P450LiverFunctional significanceCo-transfection experimentsBinding proteinPromoter activityExpressionReporter constructsFactorsFactor family
2003
Genetic Variability at the Human FMO1 Locus: Significance of a Basal Promoter Yin Yang 1 Element Polymorphism (FMO1*6)
Hines R, Luo Z, Hopp K, Cabacungan E, Koukouritaki S, McCarver D. Genetic Variability at the Human FMO1 Locus: Significance of a Basal Promoter Yin Yang 1 Element Polymorphism (FMO1*6). Journal Of Pharmacology And Experimental Therapeutics 2003, 306: 1210-1218. PMID: 12829732, DOI: 10.1124/jpet.103.053686.Peer-Reviewed Original ResearchConceptsSingle nucleotide polymorphismsFlavin-containing monooxygenasesGenetic variabilityIntron 1 splice donor siteElectrophoretic mobility shift assaysYin Yang 1 (YY1) transcription factorTransient expression assaysCore binding sequenceATG start codonMobility shift assaysSplice donor siteCommon single nucleotide polymorphismsYY1 bindingStructural geneTranscription factorsStart codonShift assaysExonic sequencesChromosome 1q23Binding sequenceExpression assaysPromoter activityVariety of toxicantsBase pairsNucleotide polymorphisms
2001
Molecular regulation of genes encoding xenobiotic-metabolizing enzymes: mechanisms involving endogenous factors.
Hines R, Luo Z, Cresteil T, Ding X, Prough R, Fitzpatrick J, Ripp S, Falkner K, Ge N, Levine A, Elferink C. Molecular regulation of genes encoding xenobiotic-metabolizing enzymes: mechanisms involving endogenous factors. Drug Metabolism And Disposition 2001, 29: 623-33. PMID: 11302926.Peer-Reviewed Original ResearchConceptsDevelopmental-specific expressionSignal transduction pathwaysMolecular regulationTransduction pathwaysCommon signal transduction pathwayReceptor-mediated signal transduction pathwaysRegulation of xenobioticGenome levelTranscription factorsMolecular mechanismsMolecular biologyEndogenous factorsGenesRegulatory systemXenobiotic-metabolizing enzymesEnzymeRegulationExpressionCytochrome P450Fundamental rolePathwayRapid proliferationTissueBiologyExperimental therapeutics
1996
Identification of tissue-specific DNase I hypersensitive sites in the rabbit flavin-containing monooxygenase form 2 gene.
Shehin-Johnson S, Palmer K, Hines R. Identification of tissue-specific DNase I hypersensitive sites in the rabbit flavin-containing monooxygenase form 2 gene. Drug Metabolism And Disposition 1996, 24: 891-8. PMID: 8869825.Peer-Reviewed Original ResearchConceptsDNase IHypersensitive sitesTissue-specific transcription factorsLambda EMBL3 genomic libraryPolyomavirus enhancer activator 3Base pairsTissue-specific DNase I hypersensitive siteDNase I hypersensitive sitesTissue-specific DNase IEMBL3 genomic libraryPrimer extension analysisTranscription start siteClassical TATA boxTissue-specific mannerHypersensitive domainGenomic clonesGenomic libraryMethylated regionsTranscription factorsCDNA sequenceKilobase pairsStart siteTATA boxExtension analysisType II cell populations