2012
Amine Oxidases and Reductases
Hines R, Prough R. Amine Oxidases and Reductases. 2012, 1-27. DOI: 10.1002/9780470921920.edm012.Peer-Reviewed Original ResearchNatural environmental chemicalsCytochrome P450-dependent monooxygenasesP450-dependent monooxygenasesAmine oxidationVariety of compoundsParticular reactionAmine oxidaseEnvironmental chemicalsEnzymeGenesMonooxygenasesProteinReductaseEndogenous aminesVariety of drugsOxidationAminesCompoundsOxidaseReactionChemicalsVariety
2008
Regulatory Polymorphisms and their Contribution to Interindividual Differences in the Expression of Enzymes Influencing Drug and Toxicant Disposition
Hines R, Koukouritaki S, Poch M, Stephens M. Regulatory Polymorphisms and their Contribution to Interindividual Differences in the Expression of Enzymes Influencing Drug and Toxicant Disposition. Drug Metabolism Reviews 2008, 40: 263-301. PMID: 18464046, DOI: 10.1080/03602530801952682.Peer-Reviewed Original ResearchConceptsRegulatory polymorphismsRegulatory sequencesHuman proteinsSelection pressureGene expressionCo-dominant traitEvolutionary perspectiveXenobiotic metabolismEnvironmental changesToxicant dispositionUnexpected paucityCurrent understandingHeterozygous stateTraitsPolymorphismSuch variationExpressionKnowledge gapsGenesProteinFitnessSequenceMetabolismVariationDrug disposition
2007
Mechanisms Regulating Human FMO3 Transcription
Klick D, Hines R. Mechanisms Regulating Human FMO3 Transcription. Drug Metabolism Reviews 2007, 39: 419-442. PMID: 17786630, DOI: 10.1080/03602530701498612.Peer-Reviewed Original ResearchConceptsGC-box binding proteinsHepG2 cellsPromoter characterizationLiver nuclear proteinsNuclear proteinsTransient expressionFMO enzymesDevelopmental expressionBinding proteinTranscriptionAdult regulationProteinSpecific mechanismsEnzymeExpressionCellsNFYMajor roleYY1USF1Oxidative drugsHeterodimersMonooxygenasesReporterIsoforms
2005
Haplotype and functional analysis of four flavin-containing monooxygenase isoform 2 (FMO2) polymorphisms in Hispanics
Krueger S, Siddens L, Henderson M, Andreasen E, Tanguay R, Pereira C, Cabacungan E, Hines R, Ardlie K, Williams D. Haplotype and functional analysis of four flavin-containing monooxygenase isoform 2 (FMO2) polymorphisms in Hispanics. Pharmacogenetics And Genomics 2005, 15: 245-256. PMID: 15864117, PMCID: PMC1351039, DOI: 10.1097/01213011-200504000-00008.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAntithyroid AgentsDNA PrimersDNA, ComplementaryEthylenethioureaGenetic VectorsGenotypeHaplotypesHispanic or LatinoHomozygoteHumansMethimazoleMutagenesis, Site-DirectedMutationOxygenasesPharmacogeneticsPolymerase Chain ReactionPolymorphism, GeneticPolymorphism, Single NucleotidePolymorphism, Single-Stranded ConformationalTemperatureConceptsSingle nucleotide polymorphismsInactive proteinProtein variantsSingle-strand conformation polymorphismFunctional analysisActive proteinStrand conformation polymorphismNucleotide polymorphismsFunctional impactProteinAllelesHaplotype determinationConformation polymorphismMajor alleleHaplotypesPolymorphismFMO2Minor alleleFlavinVariants
2003
Trichloroethylene Decreases Heat Shock Protein 90 Interactions with Endothelial Nitric Oxide Synthase: Implications for Endothelial Cell Proliferation
Ou J, Ou Z, McCarver D, Hines R, Oldham K, Ackerman A, Pritchard K. Trichloroethylene Decreases Heat Shock Protein 90 Interactions with Endothelial Nitric Oxide Synthase: Implications for Endothelial Cell Proliferation. Toxicological Sciences 2003, 73: 90-97. PMID: 12657742, DOI: 10.1093/toxsci/kfg062.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCalcimycinCattleCell DivisionCells, CulturedEndothelial CellsEnzyme InhibitorsHSP90 Heat-Shock ProteinsIonophoresNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IIIPhosphorylationPrecipitin TestsSuperoxidesTrichloroethyleneVascular Endothelial Growth Factor AConceptsEndothelial cell proliferationCell proliferationNormal blood vessel growthHeat shock protein 90Shock protein 90Serine 1179Hsp90 interactionEndothelial cellsProtein 90Blood vessel growthENOS functionImportant roleProteinActivation stateInhibited VEGFVessel growthGrowth factorBalance of NONew insightsProliferationVascular endothelial growth factorNitric oxideEndothelial growth factorEndothelial nitric oxide synthaseEnvironmental pollutants
2000
Developmental regulation of the L-type calcium channel α1C subunit expression in heart
Leu L, O'Hara D, Cala S, Poornima I, Hines R, Marsh J. Developmental regulation of the L-type calcium channel α1C subunit expression in heart. Molecular And Cellular Biochemistry 2000, 205: 101-109. PMID: 10821427, DOI: 10.1023/a:1007013900827.Peer-Reviewed Original ResearchConceptsRNase protection assaysProtection assaysNorthern analysisΑ1C subunitSize of proteinsAlternative splicingAbundance of mRNADevelopmental regulationSmaller transcriptTranscript sizeNative proteinKD proteinMolecular massDevelopmental stagesProteinSubunitsAdult ventriclesTranscriptsPostnatal developmentAbundanceExpressionSubunit expressionCalcium channel propertiesChannel propertiesReceptor message
1995
Characterization of hepatic flavin monooxygenase from the marine teleost turbot (Scophthalmus maximus L.)
Peters L, Livingstone D, Shenin-Johnson S, Hines R, Schlenk D. Characterization of hepatic flavin monooxygenase from the marine teleost turbot (Scophthalmus maximus L.). Xenobiotica 1995, 25: 121-131. PMID: 7618340, DOI: 10.3109/00498259509061838.Peer-Reviewed Original ResearchConceptsFlavin monooxygenaseNADPH-dependent flavin monooxygenaseFMO-1Full-length cDNA probeFMO-2TMA N-oxideGene expressionMarine teleostsMature fishNorthern analysisCDNA probeXenobiotic metabolismDMA N-oxideImmunoreactive proteinOsmoregulationConversion of trimethylamineN-oxidase activityMonooxygenaseMichaelis-Menten kineticsSingle bandTurbotPiperonyl butoxideTeleostsFishProtein