1996
Identification of tissue-specific DNase I hypersensitive sites in the rabbit flavin-containing monooxygenase form 2 gene.
Shehin-Johnson S, Palmer K, Hines R. Identification of tissue-specific DNase I hypersensitive sites in the rabbit flavin-containing monooxygenase form 2 gene. Drug Metabolism And Disposition 1996, 24: 891-8. PMID: 8869825.Peer-Reviewed Original ResearchConceptsDNase IHypersensitive sitesTissue-specific transcription factorsLambda EMBL3 genomic libraryPolyomavirus enhancer activator 3Base pairsTissue-specific DNase I hypersensitive siteDNase I hypersensitive sitesTissue-specific DNase IEMBL3 genomic libraryPrimer extension analysisTranscription start siteClassical TATA boxTissue-specific mannerHypersensitive domainGenomic clonesGenomic libraryMethylated regionsTranscription factorsCDNA sequenceKilobase pairsStart siteTATA boxExtension analysisType II cell populations
1993
Specific nuclear protein binding to a negative regulatory element on the human CYP1A1 gene
Boucher P, Ruch R, Hines R. Specific nuclear protein binding to a negative regulatory element on the human CYP1A1 gene. Journal Of Biological Chemistry 1993, 268: 17384-17391. PMID: 8349622, DOI: 10.1016/s0021-9258(19)85346-2.Peer-Reviewed Original ResearchConceptsNegative regulatory activityNuclear proteinsElectrophoretic mobility shift assaysCellular trans-acting factorsTrans-acting factorsRegulatory activityStrand-specific mannerCo-transfection studiesMethylation interference experimentsMobility shift assaysNegative regulatory elementSpecific nuclear proteinCytosine-rich sequencesHuman CYP1A1 genePromoter/enhancerRegulatory elementsShift assaysTransient expressionReporter geneDNase IChimeric plasmidsCYP1A1 promoterImportant sequencesCYP1A1 genePalindromes