2001
Induction of cytochrome P450 1A1 gene expression, oxidative stress, and genotoxicity by carbaryl and thiabendazole in transfected human HepG2 and lymphoblastoid cells
Delescluse C, Ledirac N, Li R, Piechocki M, Hines R, Gidrol X, Rahmani R. Induction of cytochrome P450 1A1 gene expression, oxidative stress, and genotoxicity by carbaryl and thiabendazole in transfected human HepG2 and lymphoblastoid cells. Biochemical Pharmacology 2001, 61: 399-407. PMID: 11226373, DOI: 10.1016/s0006-2952(00)00562-1.Peer-Reviewed Original ResearchMeSH KeywordsCarbarylChloramphenicol O-AcetyltransferaseCholinesterase InhibitorsCytochrome P-450 CYP1A1DNA DamageEndoplasmic Reticulum Chaperone BiPEnzyme InductionGene ExpressionHeat-Shock ProteinsHumansMutagenicity TestsOxidative StressThiabendazoleTranscription, GeneticTransfectionTumor Cells, CulturedConceptsXenobiotic responsive elementStress genesChloramphenicol acetyl transferase (CAT) reporter geneAcetyl transferase reporter geneLevel of transcriptionTransferase reporter geneOxidative stressCell linesHuman hepatoma HepG2 cell lineHuman lymphoblastoid cell linesTranscriptional regulationHepatoma HepG2 cell lineCytochrome P450 1A1 gene expressionReporter CATTranscriptional levelReporter geneXRE sequenceLymphoblastoid cell linesGene expressionFusion constructsIntracellular signalsParental linesCYP1A1 cDNAResponse elementResponsive element
2000
Diflubenzuron, a Benzoyl-Urea Insecticide, Is a Potent Inhibitor of TCDD-Induced CYP1A1 Expression in HepG2 Cells
Ledirac N, Delescluse C, Lesca P, Piechocki M, Hines R, de Sousa G, Pralavorio M, Rahmani R. Diflubenzuron, a Benzoyl-Urea Insecticide, Is a Potent Inhibitor of TCDD-Induced CYP1A1 Expression in HepG2 Cells. Toxicology And Applied Pharmacology 2000, 164: 273-279. PMID: 10799337, DOI: 10.1006/taap.2000.8920.Peer-Reviewed Original ResearchConceptsCYP1A1 expressionEthoxyresorufin-O-deethylase (EROD) activityHepG2 cellsStrong dose-dependent decreaseDose-dependent decreaseB16 murine melanomaHuman hepatoma cells HepG2Receptor antagonismTreatment of HepG2Hepatoma cells HepG2Human CYP1A1 promoterParental HepG2 cellsCYP1A1 inducerNoncytotoxic concentrationsMurine melanomaChloramphenicol acetyltransferase (CAT) reporter geneAdditional studiesCYP1A1 inductionNorthern blot analysisCell linesBlot analysisSynthesis inhibitorH exposurePotent inhibitorAh receptor
1995
In vitro binding and functional studies comparing the human CYPIAI negative regulatory element with the orthologous sequences from rodent genes
Boucher P, Hines R. In vitro binding and functional studies comparing the human CYPIAI negative regulatory element with the orthologous sequences from rodent genes. Carcinogenesis 1995, 16: 383-392. PMID: 7859371, DOI: 10.1093/carcin/16.2.383.Peer-Reviewed Original ResearchConceptsNegative regulatory elementSpecific DNA-protein complexesDNA-protein complexesTransient expression assaysRegulatory elementsNuclear proteinsMouse fragmentsHeterologous promoterExpression assaysCell linesElectrophoretic mobility shift assaysMouse nuclear proteinMouse CYP1A1 geneSouthwestern blot analysisMobility shift assaysCYP1A1 geneNegative regulatory activitySimilar protection patternMouse hepatoma cell lineRodent cell linesCytosine-rich sequencesCytosine-rich regionsHuman CYP1A1 geneOrthologous sequencesRodent genes
1994
Restoration of aryl hydrocarbon (Ah) responsiveness in MDA-MB-231 human breast cancer cells by transient expression of the estrogen receptor
Thomsen J, Wang X, Hines R, Safe S. Restoration of aryl hydrocarbon (Ah) responsiveness in MDA-MB-231 human breast cancer cells by transient expression of the estrogen receptor. Carcinogenesis 1994, 15: 933-937. PMID: 8200098, DOI: 10.1093/carcin/15.5.933.Peer-Reviewed Original ResearchConceptsMDA-MB-231 cellsHuman breast cancer cellsMDA-MB-231 human breast cancer cellsAh-responsivenessBreast cancer cellsNM TCDDHuman estrogen receptor expression plasmidAryl hydrocarbon responsivenessCancer cellsReceptor expression plasmidAryl hydrocarbon receptorProgesterone receptorEstrogen receptorResponsive plasmidCYP1A1 gene expressionHydrocarbon receptorReceptorsCell linesCYP1A1 geneCAT activityExpression of nuclearParallel studyHuman CYP1A1 geneCellsExpression plasmid
1989
Altered Regulation of the Cytochrome P4501A1 Gene: Novel Inducer-Independent Gene Expression in Pulmonary Carcinoma Cell Lines
McLemore T, Adelberg S, Czerwinski M, Hubbard W, Yu S, Storeng R, Wood T, Hines R, Boyd M. Altered Regulation of the Cytochrome P4501A1 Gene: Novel Inducer-Independent Gene Expression in Pulmonary Carcinoma Cell Lines. Journal Of The National Cancer Institute 1989, 81: 1787-1794. PMID: 2555530, DOI: 10.1093/jnci/81.23.1787.Peer-Reviewed Original ResearchConceptsPulmonary carcinoma cell linesLung cancer cell linesCarcinoma cell linesCancer cell linesLung cancerCell linesCYP1A1 gene expressionNon-small cell linesSmall cell lung carcinomaHuman lung cancer cell linesHeavy cigarette smokersHuman pulmonary carcinoma cell linesNon-small cell lung carcinoma cell lineSmall cell lung cancer linesNon-small cellCell lung carcinomaNormal human lungCell lung cancer linesHuman lung cancerCYP1A1 mRNACell lung carcinoma cell lineLung cancer cellsAryl hydrocarbon hydroxylase activityCYP1A1 geneLung carcinoma cell line
1988
Production of growth factors related to fibroblast growth factor and platelet-derived growth factor by human embryonal carcinoma cells
Tiesman J, Meyer A, Hines R, Rizzino A. Production of growth factors related to fibroblast growth factor and platelet-derived growth factor by human embryonal carcinoma cells. In Vitro Cellular & Developmental Biology 1988, 24: 1209-1216. PMID: 3209587, DOI: 10.1007/bf02624192.Peer-Reviewed Original ResearchConceptsPlatelet-derived growth factorGrowth factorSK-Hep-1 cellsEC cellsMouse EC cellsCarcinoma cellsHuman EC cell linesNT2/D1Basic fibroblast growth factorGrowth factor productionHeparin-binding growth factorsEmbryonal carcinoma cellsEC cell linesPDGF A-chainFibroblast growth factorHuman embryonal carcinoma cellsHuman EC cellsMouse embryonal carcinoma cellsFactor productionFGFbCell linesCDNA probeSimilar salt concentrationsCellsCurrent studyInduction of cytochrome P-450IA1 gene expression in human breast tumour cell lines
Pasanen M, Stacey S, Lykkesfeldt A, Briand P, Hines R, Autrup H. Induction of cytochrome P-450IA1 gene expression in human breast tumour cell lines. Chemico-Biological Interactions 1988, 66: 223-232. PMID: 2456161, DOI: 10.1016/0009-2797(88)90073-7.Peer-Reviewed Original ResearchConceptsBreast tumor cell linesHuman breast tumor cell linesTumor cell linesTCDD treatmentCell linesDifferent breast tumor cell linesConcentrations of TCDDZR-75BT-20Cytochrome P-450IA1NM TCDDCAMA-1Basal expression levelsControl cellsExpression levelsMCF-7Northern blot analysisBlot analysisTCDDTreatmentTotal RNAP-450IA1TCDD inductionDiol metabolitesInduction