2017
Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples
Quick J, Grubaugh ND, Pullan ST, Claro IM, Smith AD, Gangavarapu K, Oliveira G, Robles-Sikisaka R, Rogers TF, Beutler NA, Burton DR, Lewis-Ximenez LL, de Jesus JG, Giovanetti M, Hill SC, Black A, Bedford T, Carroll MW, Nunes M, Alcantara LC, Sabino EC, Baylis SA, Faria NR, Loose M, Simpson JT, Pybus OG, Andersen KG, Loman NJ. Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples. Nature Protocols 2017, 12: 1261-1276. PMID: 28538739, PMCID: PMC5902022, DOI: 10.1038/nprot.2017.066.Peer-Reviewed Original Research
2016
Isolation of a Novel Insect-Specific Flavivirus from Culiseta melanura in the Northeastern United States
Misencik MJ, Grubaugh ND, Andreadis TG, Ebel GD, Armstrong PM. Isolation of a Novel Insect-Specific Flavivirus from Culiseta melanura in the Northeastern United States. Vector-Borne And Zoonotic Diseases 2016, 16: 181-190. PMID: 26807512, PMCID: PMC4779966, DOI: 10.1089/vbz.2015.1889.Peer-Reviewed Original ResearchConceptsInsect-specific flavivirusesOpen reading framePhylogenetic analysisDistinct evolutionary lineagesAmino acid sequence identityObvious geographic structureLimited genetic variationNovel insect-specific flavivirusEvolutionary lineagesHost associationsGeographic structureComplete genomeGenetic variationField-collected mosquitoesReading frameMelanura larvaeNucleotide sequenceSequence identityLarval stagesSampled populationsNortheastern United StatesGenus FlavivirusCalbertado virusNS5 geneViral sequences