2019
Non-typeable Haemophilus influenzae isolates from patients with chronic obstructive pulmonary disease contain new phase-variable modA methyltransferase alleles controlling phasevarions
Atack JM, Murphy TF, Pettigrew MM, Seib KL, Jennings MP. Non-typeable Haemophilus influenzae isolates from patients with chronic obstructive pulmonary disease contain new phase-variable modA methyltransferase alleles controlling phasevarions. Scientific Reports 2019, 9: 15963. PMID: 31685916, PMCID: PMC6828955, DOI: 10.1038/s41598-019-52429-6.Peer-Reviewed Original ResearchConceptsNon-typeable Haemophilus influenzaeChronic obstructive pulmonary diseaseObstructive pulmonary diseaseMiddle ear infectionPulmonary diseaseEar infectionsNTHi strainsHaemophilus influenzaeExacerbation of COPDCOPDMiddle earPatientsAllele distributionInfectionInfluenzaeBacterial pathogensDiseaseMultiple allelic variantsAllelic variantsGene expressionAllelesEpigenetic mechanismsLarge panelExacerbationExpression
2018
Azithromycin Pharmacodynamics against Persistent Haemophilus influenzae in Chronic Obstructive Pulmonary Disease
Tsuji BT, Fisher J, Boadi-Yeboah R, Holden PN, Sethi S, Pettigrew MM, Murphy TF. Azithromycin Pharmacodynamics against Persistent Haemophilus influenzae in Chronic Obstructive Pulmonary Disease. Antimicrobial Agents And Chemotherapy 2018, 62: 10.1128/aac.01995-17. PMID: 29180527, PMCID: PMC5786777, DOI: 10.1128/aac.01995-17.Peer-Reviewed Original ResearchConceptsChronic obstructive pulmonary disease patientsObstructive pulmonary disease patientsChronic obstructive pulmonary diseaseHollow-fiber infection modelStrains of NTHiPulmonary disease patientsObstructive pulmonary diseasePulmonary diseaseConcentration-dependent activityDisease patientsPharmacodynamic profilePharmacodynamic responseSame patientHaemophilus influenzaeInfection modelPatientsAzithromycinPersistent strainsDaysAirwayNTHiPharmacodynamicsInfluenzaeDiseaseHaemophilus
2016
Effect of Fluoroquinolones and Macrolides on Eradication and Resistance of Haemophilus influenzae in Chronic Obstructive Pulmonary Disease
Pettigrew MM, Tsuji BT, Gent JF, Kong Y, Holden PN, Sethi S, Murphy TF. Effect of Fluoroquinolones and Macrolides on Eradication and Resistance of Haemophilus influenzae in Chronic Obstructive Pulmonary Disease. Antimicrobial Agents And Chemotherapy 2016, 60: 4151-4158. PMID: 27139476, PMCID: PMC4914697, DOI: 10.1128/aac.00301-16.Peer-Reviewed Original ResearchConceptsChronic obstructive pulmonary diseaseObstructive pulmonary diseaseH. influenzae isolatesH. influenzaePulmonary diseaseFluoroquinolone administrationInfluenzae isolatesDevelopment of resistanceHaemophilus influenzaeEradication of carriageEffects of macrolidesQuinolone resistance-determining regionsMICs of antibioticsEffects of fluoroquinolonesResistance-determining regionMacrolide administrationCOPD patientsEffects of antibioticsAzithromycin MICsAntibiotic useSusceptibility breakpointsAntibiotic susceptibilityMacrolide susceptibilityInfluenzaeAdministration
2015
Epidemiological Markers for Interactions Among Streptococcus pneumoniae, Haemophilus influenzae, and Staphylococcus aureus in Upper Respiratory Tract Carriage
Lewnard JA, Givon-Lavi N, Huppert A, Pettigrew MM, Regev-Yochay G, Dagan R, Weinberger DM. Epidemiological Markers for Interactions Among Streptococcus pneumoniae, Haemophilus influenzae, and Staphylococcus aureus in Upper Respiratory Tract Carriage. The Journal Of Infectious Diseases 2015, 213: 1596-1605. PMID: 26704617, PMCID: PMC4837906, DOI: 10.1093/infdis/jiv761.Peer-Reviewed Original ResearchMeSH KeywordsCarrier StateChild, PreschoolFemaleHaemophilus InfectionsHaemophilus influenzaeHeptavalent Pneumococcal Conjugate VaccineHumansInfantIsraelLongitudinal StudiesMalePneumococcal InfectionsRespiratory Tract InfectionsRisk FactorsStaphylococcal InfectionsStaphylococcus aureusStreptococcus pneumoniaeVaccines, ConjugateConceptsPneumococcal serotype replacementH. influenzaeSerotype replacementS. pneumoniaeHaemophilus influenzaeStreptococcus pneumoniaePneumococcal conjugate vaccine trialUpper respiratory tract carriagePneumococcal vaccine introductionS. aureus carriageMonths of ageStaphylococcus aureusPCV7 recipientsAureus carriageVaccine introductionVaccine trialsCarriage patternsConfounding factorsClinical interventionsInfluenzaeEpidemiological markersBedouin childrenPneumoniaeCarriageS. aureusExpression of IgA Proteases by Haemophilus influenzae in the Respiratory Tract of Adults With Chronic Obstructive Pulmonary Disease
Murphy TF, Kirkham C, Jones MM, Sethi S, Kong Y, Pettigrew MM. Expression of IgA Proteases by Haemophilus influenzae in the Respiratory Tract of Adults With Chronic Obstructive Pulmonary Disease. The Journal Of Infectious Diseases 2015, 212: 1798-1805. PMID: 25995193, PMCID: PMC4633762, DOI: 10.1093/infdis/jiv299.Peer-Reviewed Original ResearchConceptsChronic obstructive pulmonary diseaseObstructive pulmonary diseaseH. influenzaeIgA protease genePulmonary diseaseHuman airwaysHaemophilus influenzaeIgA proteaseH. influenzae infectionsHuman respiratory epithelial cellsHuman IgA1Respiratory epithelial cellsInfluenzae infectionRespiratory tractIgA protease activitySputum samplesClinical settingInfluenzaeEpithelial cellsInfectionAirwayIgA1 fragmentsDiseaseIgA1Protease gene
2009
Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis Media Causing Pathogens
Thomas JC, Pettigrew MM. Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis Media Causing Pathogens. Methods In Molecular Biology 2009, 493: 179-190. PMID: 18839348, PMCID: PMC3581352, DOI: 10.1007/978-1-59745-523-7_11.Peer-Reviewed Original ResearchConceptsOtitis mediaMolecular epidemiologyMultilocus sequence typingMoraxella catarrhalisHaemophilus influenzaeStreptococcus pneumoniaeSequence typingPulsed-Field Gel ElectrophoresisEpidemiologyBacterial pathogensField gel electrophoresisType of studyResearch settingsCharacterization of isolatesPathogensCatarrhalisInfluenzaePneumoniae
2008
Microbial Interactions during Upper Respiratory Tract Infections - Volume 14, Number 10—October 2008 - Emerging Infectious Diseases journal - CDC
Pettigrew MM, Gent JF, Revai K, Patel JA, Chonmaitree T. Microbial Interactions during Upper Respiratory Tract Infections - Volume 14, Number 10—October 2008 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2008, 14: 1584-1591. PMID: 18826823, PMCID: PMC2609881, DOI: 10.3201/eid1410.080119.Peer-Reviewed Original ResearchMeSH KeywordsChild, PreschoolCohort StudiesFemaleHaemophilus InfectionsHaemophilus influenzaeHumansInfantMaleMoraxella catarrhalisMoraxellaceae InfectionsNasopharynxOtitis MediaProspective StudiesRespiratory Tract InfectionsStaphylococcal InfectionsStaphylococcus aureusStreptococcus pneumoniaeSuperinfectionConceptsUpper respiratory tract infectionH. influenzaeInfectious Diseases journal - CDCRespiratory tract infectionsS. pneumoniae colonizationHealthy children 6Months of ageS. aureusProspective cohortTract infectionsPneumoniae colonizationVaccination strategiesAntimicrobial therapyMoraxella catarrhalisChildren 6M. catarrhalisS. pneumoniaeHaemophilus influenzaeStreptococcus pneumoniaeBacterial infectionsSpecific bacterial speciesInfluenzaeLikelihood of colonizationCatarrhalisStaphylococcus aureus
2004
Prevalence of the hifBC, hmw1A, hmw2A, hmwC, and hia Genes in Haemophilus influenzae Isolates
Ecevit IZ, McCrea KW, Pettigrew MM, Sen A, Marrs CF, Gilsdorf JR. Prevalence of the hifBC, hmw1A, hmw2A, hmwC, and hia Genes in Haemophilus influenzae Isolates. Journal Of Clinical Microbiology 2004, 42: 3065-3072. PMID: 15243061, PMCID: PMC446296, DOI: 10.1128/jcm.42.7.3065-3072.2004.Peer-Reviewed Original ResearchConceptsAcute otitis mediaOtitis mediaH. influenzaeHia geneHaemophilus influenzaeMiddle earRespiratory tract sitesH. influenzae infectionsAdhesin genesClinical H. influenzaeThroat isolatesInfluenzae infectionNTHi isolatesNasopharyngeal colonizationNTHi strainsType b strainsClinical isolatesPrevalenceInfluenzaeB strainsEpithelial cellsHMW adhesinsType BVirulence factorsHibSupplementary figure: Diversity and sharing of Haemophilus influenzae strains colonizing healthy children attending day-care centers
FARJO R, FOXMAN B, PATEL M, ZHANG L, PETTIGREW M, MCCOY S, MARRS C, GILSDORF J. Supplementary figure: Diversity and sharing of Haemophilus influenzae strains colonizing healthy children attending day-care centers. The Pediatric Infectious Disease Journal 2004, 23: e2. DOI: 10.1097/00006454-200401000-00014.Peer-Reviewed Original ResearchDiversity and sharing of Haemophilus influenzae strains colonizing healthy children attending day-care centers
FARJO RS, FOXMAN B, PATEL MJ, ZHANG L, PETTIGREW MM, MCCOY SI, MARRS CF, GILSDORF JR. Diversity and sharing of Haemophilus influenzae strains colonizing healthy children attending day-care centers. The Pediatric Infectious Disease Journal 2004, 23: 41-46. PMID: 14743045, DOI: 10.1097/01.inf.0000106981.89572.d1.Peer-Reviewed Original ResearchMeSH KeywordsCarrier StateChild Day Care CentersChild, PreschoolCohort StudiesColony Count, MicrobialDNA, BacterialElectrophoresis, Gel, Pulsed-FieldFemaleGenotypeHaemophilus InfectionsHaemophilus influenzaeHumansIncidenceMaleMass ScreeningMichiganOtitis MediaPolymerase Chain ReactionReference ValuesRisk AssessmentSerotypingConceptsDay care centersH. influenzaeHealthy childrenH. influenzae organismsAcute otitis mediaH. influenzae colonizationH. influenzae strainsUnique strainsEnterobacterial repetitive intergenic consensus PCRThroat culturesOtitis mediaInitial screening techniqueNasopharyngeal colonizationPrevalence ratesColonizing strainsHaemophilus influenzaeHeterogeneity of strainInfluenzaeConsensus PCRRate of colonizationChildrenField gel electrophoresisSame strainPathogenic microorganismsEvidence of sharing
2002
Identification of the Lipooligosaccharide Biosynthesis Gene lic2B as a Putative Virulence Factor in Strains of Nontypeable Haemophilus influenzae That Cause Otitis Media
Pettigrew M, Foxman B, Marrs C, Gilsdorf J. Identification of the Lipooligosaccharide Biosynthesis Gene lic2B as a Putative Virulence Factor in Strains of Nontypeable Haemophilus influenzae That Cause Otitis Media. Infection And Immunity 2002, 70: 3551-3556. PMID: 12065495, PMCID: PMC128108, DOI: 10.1128/iai.70.7.3551-3556.2002.Peer-Reviewed Original ResearchConceptsMiddle ear isolatesNT H. influenzaeOtitis mediaH. influenzaeEar isolatesThroat isolatesHaemophilus influenzaeMiddle earAcute otitis mediaDay careNontypeable Haemophilus influenzaeVirulence factorsSpecific virulence factorsType B isolatesOM pathogenesisPutative virulence factorsH. influenzae geneImportant causeClinical isolatesPathogenic processesInfluenzaeB isolatesAcid levelsAmino acid levelsNontypeableUse of Pulsed-Field Gel Electrophoresis, Enterobacterial Repetitive Intergenic Consensus Typing, and Automated Ribotyping To Assess Genomic Variability among Strains of Nontypeable Haemophilus influenzae
Pettigrew M, Foxman B, Ecevit Z, Marrs C, Gilsdorf J. Use of Pulsed-Field Gel Electrophoresis, Enterobacterial Repetitive Intergenic Consensus Typing, and Automated Ribotyping To Assess Genomic Variability among Strains of Nontypeable Haemophilus influenzae. Journal Of Clinical Microbiology 2002, 40: 660-662. PMID: 11825990, PMCID: PMC153392, DOI: 10.1128/jcm.40.2.660-662.2002.Peer-Reviewed Original Research