2022
Culture-Independent Detection and Identification of Leptospira Serovars
Matthias M, Lubar A, Acharige S, Chaiboonma K, Pilau N, Marroquin A, Jayasundara D, Agampodi S, Vinetz J. Culture-Independent Detection and Identification of Leptospira Serovars. Microbiology Spectrum 2022, 10: e02475-22. PMID: 36445143, PMCID: PMC9769591, DOI: 10.1128/spectrum.02475-22.Peer-Reviewed Original ResearchConceptsPublic health importanceProspective studyFatal diseaseHealth importanceOutbreak investigationSequence typingDog urine samplesCulture-independent assaysEnvironmental surveillance studyMultilocus sequence typingPulsed-field gel electrophoresisSurveillance studyGroup IIHuman leptospirosisPrognostic testSpecific real-time qPCRClinical settingGroup ILeptospira serovarsReal-time qPCRDiagnostic testsSerum samplesUrine samplesDiagnostic specificityLeptospirosisDiagnostic method-based underestimation of leptospirosis in clinical and research settings; an experience from a large prospective study in a high endemic setting
Warnasekara J, Srimantha S, Kappagoda C, Jayasundara D, Senevirathna I, Matthias M, Agampodi S, Vinetz J. Diagnostic method-based underestimation of leptospirosis in clinical and research settings; an experience from a large prospective study in a high endemic setting. PLOS Neglected Tropical Diseases 2022, 16: e0010331. PMID: 35377883, PMCID: PMC9009773, DOI: 10.1371/journal.pntd.0010331.Peer-Reviewed Original ResearchConceptsMicroscopic agglutination testProspective hospital-based studyLeptospirosis casesQuantitative PCRHigh endemic settingHospital-based studyClinical case definitionLarge prospective studiesDisease burden estimatesPublic health burdenStandard diagnostic testMonths of observationUndifferentiated feverAcute leptospirosisProspective studyCase definitionEndemic settingsHealth burdenTimely diagnosisBurden estimatesDiagnostic valueSignificant human mortalityAgglutination testStudy settingDiagnostic tests
2021
12 Novel clonal groups of Leptospira infecting humans in multiple contrasting epidemiological contexts in Sri Lanka
Jayasundara D, Senavirathna I, Warnasekara J, Gamage C, Siribaddana S, Kularatne SAM, Matthias M, Mariet JF, Picardeau M, Agampodi S, Vinetz J. 12 Novel clonal groups of Leptospira infecting humans in multiple contrasting epidemiological contexts in Sri Lanka. PLOS Neglected Tropical Diseases 2021, 15: e0009272. PMID: 33735202, PMCID: PMC8009393, DOI: 10.1371/journal.pntd.0009272.Peer-Reviewed Original ResearchConceptsClonal groupsAcute kidney injuryL. interrogansMajor clinical challengeGlobal disease threatsLeptospira speciesL. interrogans infectionKidney injuryLiver impairmentCardiovascular involvementL. borgpeterseniiClinical presentationProspective studyClinical severityClinical challengePathogenic Leptospira speciesAnnual epidemicsUbiquitous zoonotic diseaseNew serogroupEpidemiological contextZoonotic diseaseCulture isolationLeptospiral culturesL. weiliiLeptospira diversity