2024
A simple, field-applicable method to increase the infectivity of wild isolates of Plasmodium falciparum to mosquito vectors
Ouattara S, Hien D, Nao E, Paré P, Guissou E, Cohuet A, Morlais I, Yerbanga R, Dabiré K, Ouédraogo J, Mouline K, Lefèvre T. A simple, field-applicable method to increase the infectivity of wild isolates of Plasmodium falciparum to mosquito vectors. Malaria Journal 2024, 23: 135. PMID: 38711028, PMCID: PMC11075210, DOI: 10.1186/s12936-024-04969-0.Peer-Reviewed Original ResearchConceptsDirect membrane feeding assaysGametocyte densityHuman-to-mosquito transmissionDonor bloodLow gametocyte densitiesMembrane feeding assaysPlasmodium falciparum gametocytesTransmission-blocking interventionsWhole bloodInfection rateFalciparum gametocytesInfectious reservoirNaive serumAsymptomatic individualsMosquito infectionHuman donorsRemoval of plasmaEndemic countriesSerumPlasmodiumParasite isolatesInfectionBlood treatmentGametocytesPlasma volume
2017
Detection of Plasmodium falciparum male and female gametocytes and determination of parasite sex ratio in human endemic populations by novel, cheap and robust RTqPCR assays
Santolamazza F, Avellino P, Siciliano G, Yao F, Lombardo F, Ouédraogo J, Modiano D, Alano P, Mangano V. Detection of Plasmodium falciparum male and female gametocytes and determination of parasite sex ratio in human endemic populations by novel, cheap and robust RTqPCR assays. Malaria Journal 2017, 16: 468. PMID: 29149898, PMCID: PMC5693539, DOI: 10.1186/s12936-017-2118-z.Peer-Reviewed Original ResearchConceptsRatio of maleFalciparum gametocytesEpidemiological surveyRTqPCR assaysFemale gametocytesLarge-scale epidemiological surveysP. falciparum gametocytesPlasmodium falciparum gametocytesRobust molecular assaySubmicroscopic infectionsGametocyte sex ratioPeripheral bloodBlood samplesEndemic areasNovel markerReal-time qPCRMalaria parasitesPfs25TaqMan assaysGametocytesMolecular assaysHuman subjectsTime qPCRMale gametocytesParasite sex ratio