2000
Survival of Ehrlichia chaffeensis in refrigerated, ADSOL‐treated RBCs
McKechnie D, Slater K, Childs J, Massung R, Paddock C. Survival of Ehrlichia chaffeensis in refrigerated, ADSOL‐treated RBCs. Transfusion 2000, 40: 1041-1047. PMID: 10988303, DOI: 10.1046/j.1537-2995.2000.40091041.x.Peer-Reviewed Original ResearchConceptsE. chaffeensisNucleated cellsTransfusion-acquired infectionEhrlichia chaffeensisMonocytic cell line THP-1Cell line THP-1Infected cell linesCytocentrifuged slidesTransfusion transmissionPacked RBC unitsPCR positivityE. chaffeensis DNASupernatant fractionCell culturesWeekly examinationsRBC unitsStudy designRBC productsTHP-1DH82 cellsPCR resultsCell linesChaffeensisInfectionSuccessful reisolation
1999
Molecular Cloning and Characterization of the Ehrlichia chaffeensis Variable-Length PCR Target: an Antigen-Expressing Gene That Exhibits Interstrain Variation
Sumner J, Childs J, Paddock C. Molecular Cloning and Characterization of the Ehrlichia chaffeensis Variable-Length PCR Target: an Antigen-Expressing Gene That Exhibits Interstrain Variation. Journal Of Clinical Microbiology 1999, 37: 1447-1453. PMID: 10203503, PMCID: PMC84798, DOI: 10.1128/jcm.37.5.1447-1453.1999.Peer-Reviewed Original ResearchConceptsOpen reading frameAmino acid sequenceApparent molecular massE. chaffeensisGenomic libraryMolecular cloningReading frameAcid sequenceDifferentiation of isolatesPCR targetsClose homologyMolecular massNucleotide sequencingImmunoreactive proteinDifferent isolatesDNA samplesInterstrain variationGenesRepeat unitsMolecular databasesCell culturesAmblyomma americanum ticksSequencePCR detectionChaffeensis