2013
SERUM ANTIBODIES TO BORRELIA BURGDORFERI, ANAPLASMA PHAGOCYTOPHILUM, AND BABESIA MICROTI IN RECAPTURED WHITE-FOOTED MICE
Magnarelli LA, Williams SC, Norris SJ, Fikrig E. SERUM ANTIBODIES TO BORRELIA BURGDORFERI, ANAPLASMA PHAGOCYTOPHILUM, AND BABESIA MICROTI IN RECAPTURED WHITE-FOOTED MICE. Journal Of Wildlife Diseases 2013, 49: 294-302. PMID: 23568904, PMCID: PMC3712766, DOI: 10.7589/2012-06-172.Peer-Reviewed Original ResearchConceptsB. microtiA. phagocytophilumB. burgdorferiBabesia microtiSolid-phase enzyme-linked immunosorbent assayAnaplasma phagocytophilumMouse immune systemEnzyme-linked immunosorbent assayIndirect fluorescent antibodyAntibody statusAntibody titersBorrelia burgdorferi sensu strictoSerum antibodiesTotal antibodyAntibody prevalenceNegative statusBurgdorferi sensu strictoImmune systemPatent infectionsEnd pointMiceImmunosorbent assayAntibodiesBorrelia burgdorferiFluorescent antibody
2010
SEASONAL PREVALENCE OF SERUM ANTIBODIES TO WHOLE CELL AND RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI AND ANAPLASMA PHAGOCYTOPHILUM IN WHITE-TAILED DEER IN CONNECTICUT
Magnarelli LA, Williams SC, Fikrig E. SEASONAL PREVALENCE OF SERUM ANTIBODIES TO WHOLE CELL AND RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI AND ANAPLASMA PHAGOCYTOPHILUM IN WHITE-TAILED DEER IN CONNECTICUT. Journal Of Wildlife Diseases 2010, 46: 781-790. PMID: 20688684, DOI: 10.7589/0090-3558-46.3.781.Peer-Reviewed Original ResearchConceptsRecombinant antigensInfected Ixodes scapularis ticksCoexistence of antibodiesAnaplasma phagocytophilumRespective causative agentsTick-borne infectionsEnzyme-linked immunosorbent assayIndirect fluorescent antibodyHuman granulocytic anaplasmosisPolyvalent enzyme-linked immunosorbent assaysIxodes scapularis ticksWhole blood samplesAntibody titersCurrent infectionWhole-cell B. burgdorferiBorrelia burgdorferi sensu strictoSerum antibodiesTotal antibodyBlood samplesHigher antibodyBurgdorferi sensu strictoSpecific recombinant antigensLyme borreliosisDeer seraImmunosorbent assay
2006
ANTIBODIES TO WHOLE-CELL OR RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI, ANAPLASMA PHAGOCYTOPHILUM, AND BABESIA MICROTI IN WHITE-FOOTED MICE
Magnarelli LA, Stafford KC, IJdo JW, Fikrig E. ANTIBODIES TO WHOLE-CELL OR RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI, ANAPLASMA PHAGOCYTOPHILUM, AND BABESIA MICROTI IN WHITE-FOOTED MICE. Journal Of Wildlife Diseases 2006, 42: 732-738. PMID: 17255439, DOI: 10.7589/0090-3558-42.4.732.Peer-Reviewed Original ResearchMeSH KeywordsAnaplasma phagocytophilumAnimalsAnimals, WildAntibodies, BacterialAntibodies, ProtozoanAntigens, BacterialAntigens, ProtozoanBabesia microtiBabesiosisBorrelia burgdorferiConnecticutEhrlichiosisEnzyme-Linked Immunosorbent AssayLyme DiseasePeromyscusRodent DiseasesSeroepidemiologic StudiesTick-Borne DiseasesConceptsEnzyme-linked immunosorbent assayB. burgdorferiOverall antibody prevalence rateBabesia microtiBorrelia burgdorferiAntibody prevalence ratesAnaplasma phagocytophilumA. phagocytophilumIndirect fluorescent antibodyWhite-footed miceWhole cell antigenTick-infested areasWhole-cell B. burgdorferiCurrent infectionPrevalence ratesMouse serumAntigenImmunosorbent assaySerum samplesRecombinant antigensAntibodiesSerumB. microtiMiceFluorescent antibody
2004
A comparison of serologic tests for the detection of serum antibodies to whole-cell and recombinant Borrelia burgdorferi antigens in cattle.
Magnarelli LA, Bushmich SL, Sherman BA, Fikrig E. A comparison of serologic tests for the detection of serum antibodies to whole-cell and recombinant Borrelia burgdorferi antigens in cattle. Canadian Veterinary Journal 2004, 45: 667-73. PMID: 15368740, PMCID: PMC546445.Peer-Reviewed Original ResearchMeSH KeywordsAnaplasma phagocytophilumAnimalsAntibodies, BacterialAntigens, BacterialBlotting, WesternBorrelia burgdorferiCattleCattle DiseasesConnecticutEnzyme-Linked Immunosorbent AssayFemaleFluorescent Antibody Technique, IndirectLyme DiseaseMaleMolecular WeightRecombinant ProteinsReproducibility of ResultsSensitivity and SpecificityTick InfestationsConceptsPolyvalent enzyme-linked immunosorbentVlsE antigenBorrelia burgdorferi antigensRecombinant antigensAntibody staining methodsHealthy dairyB. burgdorferi antigensIndirect fluorescent-antibody staining methodsCattle seraHigh specificityLeptospira interrogans serovarsBrucella spWhole cell antigenSerum samplesWestern blot procedureP39 antigenUSE OF RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI AND ANAPLASMA PHAGOCYTOPHILUM IN ENZYME-LINKED IMMUNOSORBENT ASSAYS TO DETECT ANTIBODIES IN WHITE-TAILED DEER
Magnarelli LA, IJdo JW, Ramakrishnan U, Henderson DW, Stafford KC, Fikrig E. USE OF RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI AND ANAPLASMA PHAGOCYTOPHILUM IN ENZYME-LINKED IMMUNOSORBENT ASSAYS TO DETECT ANTIBODIES IN WHITE-TAILED DEER. Journal Of Wildlife Diseases 2004, 40: 249-258. PMID: 15362824, DOI: 10.7589/0090-3558-40.2.249.Peer-Reviewed Original Research
2002
Comparative reactivity of human sera to recombinant VlsE and other Borrelia burgdorferi antigens in class-specific enzyme-linked immunosorbent assays for Lyme borreliosis
Magnarelli LA, Lawrenz M, Norris SJ, Fikrig E. Comparative reactivity of human sera to recombinant VlsE and other Borrelia burgdorferi antigens in class-specific enzyme-linked immunosorbent assays for Lyme borreliosis. Journal Of Medical Microbiology 2002, 51: 649-655. PMID: 12171295, DOI: 10.1099/0022-1317-51-8-649.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayIgG antibodiesErythema migransIgM antibodiesLyme borreliosisPhysician-diagnosed erythema migransOnset of illnessVlsE antigenB. burgdorferi infectionBorrelia burgdorferi antigensEnzyme-linked immunosorbentHuman granulocytic ehrlichiosisHuman serumOuter surface protein CRheumatoid arthritisBorrelia burgdorferi sensu strictoOral infectionBurgdorferi infectionFalse-positive reactionsBurgdorferi sensu strictoSera crossLouse-borneLaboratory diagnosisNormal serumGranulocytic ehrlichiosisAntibodies to granulocytic ehrlichiae in cattle from Connecticut
Magnarelli LA, Ijdo JW, Sherman B, Bushmich SL, Levy SA, Fikrig E. Antibodies to granulocytic ehrlichiae in cattle from Connecticut. Journal Of Medical Microbiology 2002, 51: 326-331. PMID: 11926738, DOI: 10.1099/0022-1317-51-4-326.Peer-Reviewed Original ResearchConceptsIndirect fluorescent antibody
2001
Reactivity of dog sera to whole-cell or recombinant antigens of Borrelia burgdorferi by ELISA and immunoblot analysis
Magnarelli LA, Levy SA, Ijdo JW, Wu C, Padula SJ, Fikrig E. Reactivity of dog sera to whole-cell or recombinant antigens of Borrelia burgdorferi by ELISA and immunoblot analysis. Journal Of Medical Microbiology 2001, 50: 889-895. PMID: 11599738, DOI: 10.1099/0022-1317-50-10-889.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayWhole cell antigenRecombinant antigensB. burgdorferiNon-vaccinated dogsPolyvalent enzyme-linked immunosorbent assaysCanine borreliosisTick-infested areasTotal immunoglobulinWhole-cell B. burgdorferiAntibody titresBorrelia burgdorferi sensu strictoVaccinated dogsJoint disordersImmune responseBurgdorferi sensu strictoAntigenImmunosorbent assaySerumDogsNatural infectionReactivity of serum samples of dogs and horses tested by use of class-specific recombinant-based enzyme-linked immunosorbent assays for detection of granulocytic ehrlichiosis.
Magnarelli LA, Ijdo JW, Van Andel AE, Wu C, Oliver JH, Fikrig E. Reactivity of serum samples of dogs and horses tested by use of class-specific recombinant-based enzyme-linked immunosorbent assays for detection of granulocytic ehrlichiosis. American Journal Of Veterinary Research 2001, 62: 1365-9. PMID: 11560261, DOI: 10.2460/ajvr.2001.62.1365.Peer-Reviewed Original ResearchConceptsIgG antibodiesSerum samplesHGE agentGranulocytic ehrlichiosisClient-owned dogsDog serum samplesHuman granulocytic ehrlichiosis (HGE) agentEnzyme-linked immunosorbentPositive resultsWestern blot analysisTick-infested areasEhrlichia phagocytophila genogroupTotal immunoglobulinHorse serum samplesPromyelocytic leukemia cellsClinical relevanceHuman promyelocytic leukemia cellsProtein antigensIgMELISADogsLeukemia cellsAntibodiesBlot analysisAntigen
2000
Detection of Ehrlichia chaffeensis DNA inAmblyomma americanum Ticks in Connecticut and Rhode Island
Ijdo J, Wu C, Magnarelli L, Stafford K, Anderson J, Fikrig E. Detection of Ehrlichia chaffeensis DNA inAmblyomma americanum Ticks in Connecticut and Rhode Island. Journal Of Clinical Microbiology 2000, 38: 4655-4656. PMID: 11101616, PMCID: PMC87657, DOI: 10.1128/jcm.38.12.4655-4656.2000.Peer-Reviewed Original ResearchThe Emergence of Another Tickborne Infection in the 12-Town Area around Lyme, Connecticut: Human Granulocytic Ehrlichiosis
IJdo J, Meek J, Cartter M, Magnarelli L, Wu C, Tenuta S, Fikrig E, Ryder R. The Emergence of Another Tickborne Infection in the 12-Town Area around Lyme, Connecticut: Human Granulocytic Ehrlichiosis. The Journal Of Infectious Diseases 2000, 181: 1388-1393. PMID: 10751139, DOI: 10.1086/315389.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisTickborne infectionProspective population-based surveillanceGranulocytic ehrlichiosisCommon tickborne infectionPopulation-based surveillancePrimary care providersSubset of seraIndirect fluorescent antibody methodIllness suggestiveCare providersLaboratory evidenceImportant causeProbable casesFluorescent antibody methodImmunoblot assayLyme diseaseInfectionAntibody methodIncidenceEhrlichiosisLymeMorbidityDiseaseSuggestive
1999
ANTIBODIES TO GRANULOCYTIC EHRLICHIAE IN WHITE-FOOTED AND COTTON MICE IN EASTERN UNITED STATES
Magnarelli L, Stafford K, IJdo J, Fikrig E, Oliver J, Hutcheson H, Boone J. ANTIBODIES TO GRANULOCYTIC EHRLICHIAE IN WHITE-FOOTED AND COTTON MICE IN EASTERN UNITED STATES. Journal Of Wildlife Diseases 1999, 35: 259-265. PMID: 10231752, DOI: 10.7589/0090-3558-35.2.259.Peer-Reviewed Original ResearchConceptsIndirect fluorescent antibodyNCH-1 strainHuman granulocytic ehrlichiosis (HGE) agentGranulocytic ehrlichiaeWestern blot analysisP. gossypinusSerologic assaysImmune responseWestern blot procedureEtiologic agentMouse serumSurveillance programSerum samplesMiceSerumAntibodiesEndemic sitesFluorescent antibodyBlot analysisWhite-footed miceIFA methodTitersEhrlichiaeP. leucopusAmelia IslandINFECTIONS OF GRANULOCYTIC EHRLICHIAE AND BORRELIA BURGDORFERI IN WHITE-TAILED DEER IN CONNECTICUT
Magnarelli L, IJdo J, Stafford K, Fikrig E. INFECTIONS OF GRANULOCYTIC EHRLICHIAE AND BORRELIA BURGDORFERI IN WHITE-TAILED DEER IN CONNECTICUT. Journal Of Wildlife Diseases 1999, 35: 266-274. PMID: 10231753, DOI: 10.7589/0090-3558-35.2.266.Peer-Reviewed Original Research
1998
Reactivity of Human Sera to Different Strains of Granulocytic Ehrlichiae in Immunodiagnostic Assays
Magnarelli L, IJdo J, Dumler J, Heimer R, Fikrig E. Reactivity of Human Sera to Different Strains of Granulocytic Ehrlichiae in Immunodiagnostic Assays. The Journal Of Infectious Diseases 1998, 178: 1835-1838. PMID: 9815246, DOI: 10.1086/314516.Peer-Reviewed Original ResearchConceptsNCH-1 strainIndirect IFAHuman granulocytic ehrlichiosis (HGE) agentHuman granulocytic ehrlichiosisWestern blot analysisInfected neutrophilsPromyelocytic leukemia cellsHuman promyelocytic leukemia cellsAntibody detectionGranulocytic ehrlichiosisEhrlichia equiDifferent strainsHuman isolatesLeukemia cellsSerumImmunodiagnostic assaysBlot analysisGranulocytic ehrlichiaeIFA analysisHuman serumIFAEhrlichiaeAssaysSeropositivityPatientsHuman Exposure to a Granulocytic Ehrlichia and Other Tick-Borne Agents in Connecticut
Magnarelli L, Ijdo J, Anderson J, Padula S, Flavell R, Fikrig E. Human Exposure to a Granulocytic Ehrlichia and Other Tick-Borne Agents in Connecticut. Journal Of Clinical Microbiology 1998, 36: 2823-2827. PMID: 9738027, PMCID: PMC105071, DOI: 10.1128/jcm.36.10.2823-2827.1998.Peer-Reviewed Original ResearchConceptsHuman monocytic ehrlichiosisWestern blot analysisTick-borne agentsProbable human casesTick-borne infectionsHuman granulocytic ehrlichiosis (HGE) agentBlot analysisSerologic testingMarked leukopeniaSeropositive specimensPositive seraHGE agentLyme borreliosisBabesia microtiHuman casesMonocytic ehrlichiosisEhrlichia equiBorrelia burgdorferiGranulocytic EhrlichiaEhrlichia chaffeensisFrequent detectionHuman exposurePatientsInfectionAntibodies
1997
Antibodies to Ehrlichia equi in dogs from the northeastern United States.
Magnarelli L, IJdo J, Anderson J, Madigan J, Dumler J, Fikrig E. Antibodies to Ehrlichia equi in dogs from the northeastern United States. Journal Of The American Veterinary Medical Association 1997, 211: 1134-7. PMID: 9364226, DOI: 10.2460/javma.1997.211.09.1134.Peer-Reviewed Original ResearchConceptsE equiIndirect fluorescent antibodyWestern blot analysisSerologic testing methodsFluorescent antibodyBlot analysisTick-infested areasSeropositive dogsIll dogsMarked leukopeniaNormal dogsSubclinical infectionSerologic analysisEtiologic agentLaboratory diagnosisEnd pointGranulocytic ehrlichiosisEhrlichia equiDogsAntibodiesHuman isolatesBorrelia burgdorferiDog serumMultiple pathogensEhrlichiosis
1995
Coexistence of antibodies to tick-borne pathogens of babesiosis, ehrlichiosis, and Lyme borreliosis in human sera
Magnarelli LA, Dumler JS, Anderson JF, Johnson RC, Fikrig E. Coexistence of antibodies to tick-borne pathogens of babesiosis, ehrlichiosis, and Lyme borreliosis in human sera. Journal Of Clinical Microbiology 1995, 33: 3054-3057. PMID: 8576376, PMCID: PMC228637, DOI: 10.1128/jcm.33.11.3054-3057.1995.Peer-Reviewed Original ResearchConceptsLyme borreliosisTick-borne agentsTick-borne pathogensSerum samplesCoexistence of antibodiesB. microti infectionIndirect fluorescent antibodyMaximal antibody titersConnecticut patientsErythema migransSerologic evidenceSerologic testingClinical pictureAntibody titersTotal immunoglobulinMicroti infectionClinical diagnosisEnd pointBabesia microtiBorrelia burgdorferiEhrlichia chaffeensisBorreliosisFluorescent antibodyAntibodiesE. chaffeensis