1997
Borrelia burgdorferi enzyme-linked immunosorbent assay for discrimination of OspA vaccination from spirochete infection
Zhang YQ, Mathiesen D, Kolbert CP, Anderson J, Schoen RT, Fikrig E, Persing DH. Borrelia burgdorferi enzyme-linked immunosorbent assay for discrimination of OspA vaccination from spirochete infection. Journal Of Clinical Microbiology 1997, 35: 233-238. PMID: 8968914, PMCID: PMC229545, DOI: 10.1128/jcm.35.1.233-238.1997.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayVaccine failureFalse positivityAntigen preparationsExperimental animalsNatural infectionLyme disease vaccineB. burgdorferiExperimental animal modelsNegative enzyme-linked immunosorbent assayEnzyme-linked immunosorbentWhole-cell sonicateSurface protein AVaccinated subjectsLyme disease casesOuter surface protein AVaccinated personsFalse-positive resultsVaccine trialsOspA vaccinationSpirochete infectionDisease vaccineAnimal modelsHuman Lyme disease casesNegative isolates
1996
Borrelia burgdorferi OspA is an arthropod-specific transmission-blocking Lyme disease vaccine.
de Silva AM, Telford SR, Brunet LR, Barthold SW, Fikrig E. Borrelia burgdorferi OspA is an arthropod-specific transmission-blocking Lyme disease vaccine. Journal Of Experimental Medicine 1996, 183: 271-275. PMID: 8551231, PMCID: PMC2192397, DOI: 10.1084/jem.183.1.271.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, BacterialAntigens, BacterialAntigens, SurfaceArachnid VectorsBacterial Outer Membrane ProteinsBacterial VaccinesBorrelia burgdorferi GroupDigestive SystemFluorescent Antibody TechniqueImmunotherapy, AdoptiveIxodesLipoproteinsLyme DiseaseMiceMice, Inbred C3HSalivary GlandsConceptsOspA antibodiesTick attachmentUnfed nymphal ticksLyme disease vaccineB. burgdorferi infectionBlood mealMechanism of protectionTick blood mealSpirochete infectionBurgdorferi infectionDisease vaccineGland invasionExpression of OspAOuter surface proteinsSpirochetal agentLyme diseaseMiceSalivary gland invasionAntibodiesSalivary glandsBorrelia burgdorferiIxodes ticksOspA expressionTick gutVaccine