2022
Use of host lipids by the Lyme disease spirochete may lead to biomarkers
Arora G, Hart T, Fikrig E. Use of host lipids by the Lyme disease spirochete may lead to biomarkers. Journal Of Clinical Investigation 2022, 132: e158254. PMID: 35289311, PMCID: PMC8920323, DOI: 10.1172/jci158254.Peer-Reviewed Original ResearchConceptsB. burgdorferi infectionLyme diseaseBurgdorferi infectionCareful clinical assessmentCommon tick-borne diseaseAnti-lipid antibodiesProduction of antibodiesTick-borne diseaseHost lipidsAntibody titersLyme disease agent Borrelia burgdorferiClinical assessmentCurrent biomarkersMurine modelDiseaseSerum samplesLyme disease spirocheteAntibodiesBorrelia burgdorferiInfectionBiomarkersPatientsLipidsSyphilisPhosphatidic acid
2019
Borrelia miyamotoi infection leads to cross-reactive antibodies to the C6 peptide in mice and men
Koetsveld J, Platonov A, Kuleshov K, Wagemakers A, Hoornstra D, Ang W, Szekeres S, van Duijvendijk G, Fikrig E, Embers M, Sprong H, Hovius J. Borrelia miyamotoi infection leads to cross-reactive antibodies to the C6 peptide in mice and men. Clinical Microbiology And Infection 2019, 26: 513.e1-513.e6. PMID: 31404672, DOI: 10.1016/j.cmi.2019.07.026.Peer-Reviewed Original ResearchConceptsCross-reactive antibodiesC6 peptideEnzyme immunoassayLyme borreliosisBorrelia miyamotoi infectionBMD patientsExperimental murine infectionC6 enzyme immunoassayAvailable immunoblotNegative immunoblotMiyamotoi infectionTick bitePatient seraMurine infectionPatientsReactivity testingInfectionVlsE proteinBorrelia miyamotoiBorrelia burgdorferiMice
2018
Rev-erb Agonist Inhibits Chikungunya and O’nyong’nyong Virus Replication
Hwang J, Jiang A, Fikrig E. Rev-erb Agonist Inhibits Chikungunya and O’nyong’nyong Virus Replication. Open Forum Infectious Diseases 2018, 5: ofy315. PMID: 30568983, PMCID: PMC6293476, DOI: 10.1093/ofid/ofy315.Peer-Reviewed Original ResearchChikungunya virusSubset of patientsRecent global epidemicJoint painStructural protein synthesisSevere inflammationInflammatory responseMultiple alphavirusesViral RNA accumulationGlobal epidemicSynthetic agonistsVirus replicationCultured murine macrophagesMurine macrophagesSR9009AlphavirusesRelated virusesVirusProtein synthesisPainInflammationPatientsAgonistsMacrophagesRNA accumulation
2016
Variable Major Proteins as Targets for Specific Antibodies against Borrelia miyamotoi
Wagemakers A, Koetsveld J, Narasimhan S, Wickel M, Deponte K, Bleijlevens B, Jahfari S, Sprong H, Karan LS, Sarksyan DS, van der Poll T, Bockenstedt LK, Bins AD, Platonov AE, Fikrig E, Hovius JW. Variable Major Proteins as Targets for Specific Antibodies against Borrelia miyamotoi. The Journal Of Immunology 2016, 196: 4185-4195. PMID: 27076681, PMCID: PMC5008243, DOI: 10.4049/jimmunol.1600014.Peer-Reviewed Original ResearchConceptsVariable major proteinsB. miyamotoiSerologic testsC3H/HeN miceBorrelia miyamotoiHumoral immune responseInfected SCID miceTick-borne relapsing feverIgM reactivityHumoral immunityHeN miceIgM productionPassive transferSCID miceSerologic assaysCurrent serologic assaysImmune responseImmune serumRelapsing feverSpecific antibodiesProtein 1MiyamotoiIxodes ticksPatientsFever spirochetes
2011
Circadian rhythms influence disease severity in a mouse model of sepsis (110.9)
Walker W, Bozzi A, Fikrig E. Circadian rhythms influence disease severity in a mouse model of sepsis (110.9). The Journal Of Immunology 2011, 186: 110.9-110.9. DOI: 10.4049/jimmunol.186.supp.110.9.Peer-Reviewed Original ResearchInflammatory responseMouse modelCircadian rhythmSerum proinflammatory cytokinesPuncture (CLP) mouse modelRisk of mortalitySeptic patientsSepsis inductionCecal ligationSepsis phenotypesEarly mortalityProinflammatory cytokinesSevere hypothermiaDisease scoreCLP modelDisease severitySepsisCreatine kinaseMortalityPatientsMiceRhythmSeverityTime 19Mammalian physiology
2004
Preferential Presence of Decorin-Binding Protein B (BBA25) and BBA50 Antibodies in Cerebrospinal Fluid of Patients with Neurologic Lyme Disease
Fikrig E, Coyle PK, Schutzer SE, Chen M, Deng Z, Flavell RA. Preferential Presence of Decorin-Binding Protein B (BBA25) and BBA50 Antibodies in Cerebrospinal Fluid of Patients with Neurologic Lyme Disease. Journal Of Clinical Microbiology 2004, 42: 1243-1246. PMID: 15004083, PMCID: PMC356844, DOI: 10.1128/jcm.42.3.1243-1246.2004.Peer-Reviewed Original Research
2002
Superoxide Anion Production during Anaplasma phagocytophila Infection
Wang T, Malawista SE, Pal U, Grey M, Meek J, Akkoyunlu M, Thomas V, Fikrig E. Superoxide Anion Production during Anaplasma phagocytophila Infection. The Journal Of Infectious Diseases 2002, 186: 274-280. PMID: 12134266, DOI: 10.1086/341451.Peer-Reviewed Original ResearchConceptsChronic granulomatous diseaseRespiratory burstA. phagocytophilaRespiratory burst inhibitionNitroblue tetrazoliumPopulation of neutrophilsSuperoxide anion productionInfected miceGranulomatous diseaseMouse modelUse of assaysPolymorphonuclear leukocytesUninfected animalsAnaplasma phagocytophilaAnion productionNeutrophilsInfectionHL-60 cellsIndividual cell basisAssaysCell basisCellsPatientsLeukocytesDisease
2001
West Nile Virus Envelope Protein
WANG T, ANDERSON J, MAGNARELLI L, BUSHMICH S, WONG S, KOSKI R, FIKRIG E. West Nile Virus Envelope Protein. Annals Of The New York Academy Of Sciences 2001, 951: 325-327. PMID: 11797789, DOI: 10.1111/j.1749-6632.2001.tb02708.x.Peer-Reviewed Original ResearchConceptsWest Nile virus envelope proteinVirus envelope proteinWest Nile virus infectionRole of antibodiesE proteinEnvelope proteinWest Nile virusPassive immunizationHumoral responseWest Nile virus E proteinVirus infectionVirus E proteinNile virusProtein serumInfectionAntibodiesRecombinant formPatientsImmunizationProteinMiceDiagnosisImmunitySerumImmunization of Mice Against West Nile Virus with Recombinant Envelope Protein
Wang T, Anderson J, Magnarelli L, Wong S, Koski R, Fikrig E. Immunization of Mice Against West Nile Virus with Recombinant Envelope Protein. The Journal Of Immunology 2001, 167: 5273-5277. PMID: 11673542, DOI: 10.4049/jimmunol.167.9.5273.Peer-Reviewed Original ResearchConceptsWN virus infectionRecombinant E proteinWest Nile virusVirus infectionC3H/HeN miceE proteinNile virusImmunization of miceRecombinant envelope proteinsPassive administrationCandidate vaccinesHeN miceFatal encephalitisImmunization experimentsImmunoblot assayWN virusInfectionVirus isolatesEnvelope proteinProtein antiserumVirusMiceEncephalitisPatientsImmunization
1999
The Immunoglobulin (IgG) Antibody Response to OspA and OspB Correlates with Severe and Prolonged Lyme Arthritis and the IgG Response to P35 Correlates with Mild and Brief Arthritis
Akin E, McHugh G, Flavell R, Fikrig E, Steere A. The Immunoglobulin (IgG) Antibody Response to OspA and OspB Correlates with Severe and Prolonged Lyme Arthritis and the IgG Response to P35 Correlates with Mild and Brief Arthritis. Infection And Immunity 1999, 67: 173-181. PMID: 9864212, PMCID: PMC96293, DOI: 10.1128/iai.67.1.173-181.1999.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAntigens, BacterialAntigens, SurfaceArthritis, InfectiousBacterial Outer Membrane ProteinsBacterial ProteinsBacterial VaccinesBorrelia burgdorferi GroupChildChild, PreschoolFemaleHumansImmunoglobulin GLipoproteinsLongitudinal StudiesLyme DiseaseMaleMiddle AgedSeverity of Illness IndexConceptsAntibody responseIgG antibodiesEarly arthritisLyme arthritisIgG responsesEarly infectionDuration of arthritisChronic Lyme arthritisIgG antibody responseSerial serum samplesBorrelia burgdorferi proteinsSubsequent arthritisB. burgdorferi proteinsSurface protein CC-terminal epitopeImmune responseArthritisSubsequent severityNatural historyLyme diseasePatientsSerum samplesImmunoglobulin GProtein CInfection
1998
Reactivity of Human Sera to Different Strains of Granulocytic Ehrlichiae in Immunodiagnostic Assays
Magnarelli L, IJdo J, Dumler J, Heimer R, Fikrig E. Reactivity of Human Sera to Different Strains of Granulocytic Ehrlichiae in Immunodiagnostic Assays. The Journal Of Infectious Diseases 1998, 178: 1835-1838. PMID: 9815246, DOI: 10.1086/314516.Peer-Reviewed Original ResearchConceptsNCH-1 strainIndirect IFAHuman granulocytic ehrlichiosis (HGE) agentHuman granulocytic ehrlichiosisWestern blot analysisInfected neutrophilsPromyelocytic leukemia cellsHuman promyelocytic leukemia cellsAntibody detectionGranulocytic ehrlichiosisEhrlichia equiDifferent strainsHuman isolatesLeukemia cellsSerumImmunodiagnostic assaysBlot analysisGranulocytic ehrlichiaeIFA analysisHuman serumIFAEhrlichiaeAssaysSeropositivityPatientsHuman Exposure to a Granulocytic Ehrlichia and Other Tick-Borne Agents in Connecticut
Magnarelli L, Ijdo J, Anderson J, Padula S, Flavell R, Fikrig E. Human Exposure to a Granulocytic Ehrlichia and Other Tick-Borne Agents in Connecticut. Journal Of Clinical Microbiology 1998, 36: 2823-2827. PMID: 9738027, PMCID: PMC105071, DOI: 10.1128/jcm.36.10.2823-2827.1998.Peer-Reviewed Original ResearchConceptsHuman monocytic ehrlichiosisWestern blot analysisTick-borne agentsProbable human casesTick-borne infectionsHuman granulocytic ehrlichiosis (HGE) agentBlot analysisSerologic testingMarked leukopeniaSeropositive specimensPositive seraHGE agentLyme borreliosisBabesia microtiHuman casesMonocytic ehrlichiosisEhrlichia equiBorrelia burgdorferiGranulocytic EhrlichiaEhrlichia chaffeensisFrequent detectionHuman exposurePatientsInfectionAntibodiesDifferential Expression of Borrelia burgdorferi Genes during Erythema Migrans and Lyme Arthritis
Fikrig E, Feng W, Aversa J, Schoen R, Flavell R. Differential Expression of Borrelia burgdorferi Genes during Erythema Migrans and Lyme Arthritis. The Journal Of Infectious Diseases 1998, 178: 1198-1201. PMID: 9806060, DOI: 10.1086/515684.Peer-Reviewed Original ResearchConceptsLyme arthritisLyme diseaseHuman infectionsPhase III clinical trialsMurine Lyme borreliosisRNA polymerase chain reactionErythema migrans biopsiesErythema migransProtective immunityClinical trialsVaccine candidatesTissue specimensLyme borreliosisPatientsGene expressionBorrelia burgdorferiDiseaseFirst direct demonstrationB. burgdorferiChain reactionArthritisInfectionBorrelia burgdorferi genesOspAB. burgdorferi gene expressionCloning of the Gene Encoding the 44-Kilodalton Antigen of the Agent of Human Granulocytic Ehrlichiosis and Characterization of the Humoral Response
Ijdo J, Sun W, Zhang Y, Magnarelli L, Fikrig E. Cloning of the Gene Encoding the 44-Kilodalton Antigen of the Agent of Human Granulocytic Ehrlichiosis and Characterization of the Humoral Response. Infection And Immunity 1998, 66: 3264-3269. PMID: 9632594, PMCID: PMC108341, DOI: 10.1128/iai.66.7.3264-3269.1998.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisGranulocytic ehrlichiosisAgent of HGESera of patientsBorrelia burgdorferi infectionMsp-2 genesElicit specific antibodiesHumoral responseBurgdorferi infectionMurine infectionHealthy individualsPatientsSerumSpecific antibodiesHL-60 cellsInfectionAntibodiesEhrlichia chaffeensisAntigenMiceEhrlichiosisGenomic DNA expression libraryMultigene familyGlutathione transferase fusion proteinFusion proteinHeat shock protein 70 of the agent of human granulocytic ehrlichiosis binds to Borrelia burgdorferi antibodies.
Ijdo J, Zhang Y, Anderson M, Goldberg D, Fikrig E. Heat shock protein 70 of the agent of human granulocytic ehrlichiosis binds to Borrelia burgdorferi antibodies. MSphere 1998, 5: 118-20. PMID: 9455892, PMCID: PMC121403, DOI: 10.1128/cdli.5.1.118-120.1998.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisEhrlichial antigenHSP-70Borrelia burgdorferi antibodiesSerologic test resultsHeat shock protein 70Shock protein 70Burgdorferi antibodiesPatient seraGranulocytic ehrlichiosisLyme diseaseFirst weekHeat shock proteinsLyme disease spirochetePrior exposureImmunoglobulin GProtein 70Immunoblot analysisAntigenShock proteinsSerumAntibodiesPatientsIllnessIgG
1997
The Early Humoral Response in Human Granulocytic Ehrlichiosis
IJdo J, Zhang Y, Hodzic E, Magnarelli L, Wilson M, Telford S, Barthold S, Fikrig E. The Early Humoral Response in Human Granulocytic Ehrlichiosis. The Journal Of Infectious Diseases 1997, 176: 687-692. PMID: 9291316, DOI: 10.1086/514091.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisAntibody responseGranulocytic ehrlichiosisHGE agentC3H/HeJ miceEarly humoral responseEarly antibody responseStrong antibody responseHumoral responseHeJ miceClinical infectionPatientsAntigenHL-60 cellsMiceInfectionIgGEhrlichiosisSerumWeeksResponseIgMAgentsDiseaseDiagnosisBorrelia burgdorferi P35 and P37 Proteins, Expressed In Vivo, Elicit Protective Immunity
Fikrig E, Barthold S, Sun W, Feng W, Telford S, Flavell R. Borrelia burgdorferi P35 and P37 Proteins, Expressed In Vivo, Elicit Protective Immunity. Immunity 1997, 6: 531-539. PMID: 9175831, DOI: 10.1016/s1074-7613(00)80341-6.Peer-Reviewed Original ResearchConceptsElicit protective immunityB. burgdorferiIgG titersProtective immunityInfected miceImmune responseHost immunityLyme borreliosisRT-PCR analysisMiceVivo-expressed antigensBorrelia burgdorferi genesImmunityP35BurgdorferiP37 proteinVivoNorthern blotAntiserumP37PatientsAntigenELISATitersBorreliosis
1995
Safety and Immunogenicity of an Outer Surface Protein A Vaccine in Subjects with Previous Lyme Disease
Schoen R, Meurice F, Brunet C, Cretella S, Krause D, Craft J, Fikrig E. Safety and Immunogenicity of an Outer Surface Protein A Vaccine in Subjects with Previous Lyme Disease. The Journal Of Infectious Diseases 1995, 172: 1324-1329. PMID: 7594671, DOI: 10.1093/infdis/172.5.1324.Peer-Reviewed Original ResearchMeSH KeywordsAdultAntibodies, BacterialAntibody FormationAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesBorrelia burgdorferi GroupDose-Response Relationship, DrugEnzyme-Linked Immunosorbent AssayHumansImmunization ScheduleImmunoglobulin GLipoproteinsLyme DiseaseProspective StudiesSafetyVaccines, SyntheticConceptsSide effectsLyme diseaseOspA vaccinePrevious Lyme diseaseThree-dose scheduleLocal side effectsSystemic side effectsLyme vaccineMild arthralgiaVaccine doseThird doseProspective studyOspA antibodiesHealthy volunteersOuter surface proteinsVaccinePatientsDiseaseImmunogenicityDoseMonthsSubjectsSurface proteinsSafetyArthralgiaA 55-kilodalton antigen encoded by a gene on a Borrelia burgdorferi 49-kilobase plasmid is recognized by antibodies in sera from patients with Lyme disease
Feng S, Das S, Lam T, Flavell RA, Fikrig E. A 55-kilodalton antigen encoded by a gene on a Borrelia burgdorferi 49-kilobase plasmid is recognized by antibodies in sera from patients with Lyme disease. Infection And Immunity 1995, 63: 3459-3466. PMID: 7642278, PMCID: PMC173477, DOI: 10.1128/iai.63.9.3459-3466.1995.Peer-Reviewed Original ResearchConceptsLate-stage Lyme diseaseEarly stage Lyme diseaseLyme diseaseIgM antibodiesSubset of patientsBorrelia burgdorferiLyme disease patientsEnzyme-linked immunosorbent assayRecombinant S1Disease patientsIgG antibodiesPulsed-field gel electrophoresisS1 antigenPatientsAntigenDiseaseSerum samplesImmunosorbent assaySerumAntibodiesB. burgdorferiRabbit antiBurgdorferiCultured spirochetesArthritis
1994
Partial destruction of Borrelia burgdorferi within ticks that engorged on OspE- or OspF-immunized mice
Nguyen TP, Lam TT, Barthold SW, Telford SR, Flavell RA, Fikrig E. Partial destruction of Borrelia burgdorferi within ticks that engorged on OspE- or OspF-immunized mice. Infection And Immunity 1994, 62: 2079-2084. PMID: 8168973, PMCID: PMC186469, DOI: 10.1128/iai.62.5.2079-2084.1994.Peer-Reviewed Original ResearchConceptsEarly-stage disease patientsMajority of patientsLate-stage diseaseSerum of miceLyme disease vaccineB. burgdorferiB. burgdorferi organismsExamination of seraNumber of spirochetesOuter surface protein ESyringe challengeDisease patientsImmune responseDisease vaccinePatientsHigh titersLyme diseaseMiceAntibodiesDiseaseSerumBurgdorferiBorreliaDaysProtein E