2009
Late Manifestations of Lyme Borreliosis
Hovius J, van Dam A, Fikrig E. Late Manifestations of Lyme Borreliosis. 2009, 9-25. DOI: 10.1128/9781555815486.ch2.Peer-Reviewed Original ResearchLyme borreliosisObjective clinical manifestationsFrequent clinical signsB. burgdorferiB. burgdorferi infectionHost immune responseCommon vector-borne diseaseClinical manifestationsErythema migransAntibiotic treatmentClinical signsBurgdorferi infectionImmune responsePositive culturesLate manifestationTick-borne pathogensLyme diseaseBorreliosisBorrelia burgdorferiDiseaseCausative agentB. afzeliiB. gariniiInfected tissuesBurgdorferi
2002
Comparative reactivity of human sera to recombinant VlsE and other Borrelia burgdorferi antigens in class-specific enzyme-linked immunosorbent assays for Lyme borreliosis
Magnarelli LA, Lawrenz M, Norris SJ, Fikrig E. Comparative reactivity of human sera to recombinant VlsE and other Borrelia burgdorferi antigens in class-specific enzyme-linked immunosorbent assays for Lyme borreliosis. Journal Of Medical Microbiology 2002, 51: 649-655. PMID: 12171295, DOI: 10.1099/0022-1317-51-8-649.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayIgG antibodiesErythema migransIgM antibodiesLyme borreliosisPhysician-diagnosed erythema migransOnset of illnessVlsE antigenB. burgdorferi infectionBorrelia burgdorferi antigensEnzyme-linked immunosorbentHuman granulocytic ehrlichiosisHuman serumOuter surface protein CRheumatoid arthritisBorrelia burgdorferi sensu strictoOral infectionBurgdorferi infectionFalse-positive reactionsBurgdorferi sensu strictoSera crossLouse-borneLaboratory diagnosisNormal serumGranulocytic ehrlichiosis
2000
Serologic Diagnosis of Lyme Borreliosis by Using Enzyme-Linked Immunosorbent Assays with Recombinant Antigens
Magnarelli L, Ijdo J, Padula S, Flavell R, Fikrig E. Serologic Diagnosis of Lyme Borreliosis by Using Enzyme-Linked Immunosorbent Assays with Recombinant Antigens. Journal Of Clinical Microbiology 2000, 38: 1735-1739. PMID: 10790090, PMCID: PMC86574, DOI: 10.1128/jcm.38.5.1735-1739.2000.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayHuman granulocytic ehrlichiosisRecombinant antigensClass-specific enzyme-linked immunosorbent assaysIgG antibodiesAntibody reactivityLyme borreliosisSerum samplesImmunoblotting of seraImmunoglobulin M antibodiesLow antibody titersB. burgdorferiEnzyme-linked immunosorbentWhole cell antigenAnalysis of serumWestern blot analysisOuter surface protein CErythema migransRheumatoid arthritisAntibody titersIgM antibodiesM antibodiesBorrelia burgdorferi sensu strictoOral infectionSurface protein C
1998
Differential Expression of Borrelia burgdorferi Genes during Erythema Migrans and Lyme Arthritis
Fikrig E, Feng W, Aversa J, Schoen R, Flavell R. Differential Expression of Borrelia burgdorferi Genes during Erythema Migrans and Lyme Arthritis. The Journal Of Infectious Diseases 1998, 178: 1198-1201. PMID: 9806060, DOI: 10.1086/515684.Peer-Reviewed Original ResearchConceptsLyme arthritisLyme diseaseHuman infectionsPhase III clinical trialsMurine Lyme borreliosisRNA polymerase chain reactionErythema migrans biopsiesErythema migransProtective immunityClinical trialsVaccine candidatesTissue specimensLyme borreliosisPatientsGene expressionBorrelia burgdorferiDiseaseFirst direct demonstrationB. burgdorferiChain reactionArthritisInfectionBorrelia burgdorferi genesOspAB. burgdorferi gene expression
1996
Use of recombinant antigens of Borrelia burgdorferi in serologic tests for diagnosis of lyme borreliosis
Magnarelli LA, Fikrig E, Padula SJ, Anderson JF, Flavell RA. Use of recombinant antigens of Borrelia burgdorferi in serologic tests for diagnosis of lyme borreliosis. Journal Of Clinical Microbiology 1996, 34: 237-240. PMID: 8788993, PMCID: PMC228775, DOI: 10.1128/jcm.34.2.237-240.1996.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assaySerum samplesRecombinant antigensPolyvalent enzyme-linked immunosorbent assaysLyme borreliosisClass-specific enzyme-linked immunosorbent assaysWhole-cell B. burgdorferiImmunoglobulin M antibodiesB. burgdorferi infectionOspC antigenErythema migransRheumatoid arthritisM antibodiesBorrelia burgdorferi sensu strictoIgG antibodiesOral infectionSerologic testsBurgdorferi infectionBurgdorferi sensu strictoPredominant reactivityAntigenOuter surface proteins OspAImmunosorbent assayBorrelia burgdorferiB. burgdorferi
1995
Coexistence of antibodies to tick-borne pathogens of babesiosis, ehrlichiosis, and Lyme borreliosis in human sera
Magnarelli LA, Dumler JS, Anderson JF, Johnson RC, Fikrig E. Coexistence of antibodies to tick-borne pathogens of babesiosis, ehrlichiosis, and Lyme borreliosis in human sera. Journal Of Clinical Microbiology 1995, 33: 3054-3057. PMID: 8576376, PMCID: PMC228637, DOI: 10.1128/jcm.33.11.3054-3057.1995.Peer-Reviewed Original ResearchConceptsLyme borreliosisTick-borne agentsTick-borne pathogensSerum samplesCoexistence of antibodiesB. microti infectionIndirect fluorescent antibodyMaximal antibody titersConnecticut patientsErythema migransSerologic evidenceSerologic testingClinical pictureAntibody titersTotal immunoglobulinMicroti infectionClinical diagnosisEnd pointBabesia microtiBorrelia burgdorferiEhrlichia chaffeensisBorreliosisFluorescent antibodyAntibodiesE. chaffeensis
1994
A chromosomal Borrelia burgdorferi gene encodes a 22-kilodalton lipoprotein, P22, that is serologically recognized in Lyme disease
Lam TT, Nguyen TP, Fikrig E, Flavell RA. A chromosomal Borrelia burgdorferi gene encodes a 22-kilodalton lipoprotein, P22, that is serologically recognized in Lyme disease. Journal Of Clinical Microbiology 1994, 32: 876-883. PMID: 8027338, PMCID: PMC263156, DOI: 10.1128/jcm.32.4.876-883.1994.Peer-Reviewed Original ResearchConceptsLate-stage diseaseLyme diseaseOuter surface proteinsEarly-stage diseaseExamination of seraErythema migransSurface proteinsPulsed-field gel electrophoresisPatientsEtiologic agentDiseaseBorrelia burgdorferiB. burgdorferiAntibodiesLipoproteinBorrelia burgdorferi genesSerumBurgdorferiShort amino terminus
1992
Comparison of whole-cell antibodies and an antigenic flagellar epitope of Borrelia burgdorferi in serologic tests for diagnosis of Lyme borreliosis
Magnarelli LA, Fikrig E, Berland R, Anderson JF, Flavell RA. Comparison of whole-cell antibodies and an antigenic flagellar epitope of Borrelia burgdorferi in serologic tests for diagnosis of Lyme borreliosis. Journal Of Clinical Microbiology 1992, 30: 3158-3162. PMID: 1280650, PMCID: PMC270607, DOI: 10.1128/jcm.30.12.3158-3162.1992.Peer-Reviewed Original ResearchConceptsWhole-cell B. burgdorferiEnzyme-linked immunosorbent assayLyme borreliosisB. burgdorferiRecombinant flagellar proteinsBorrelia burgdorferiSecond study groupFlagellar epitopesErythema migransDetectable antibodiesSerologic testsTreponemal antibodiesRecombinant proteinsStudy groupImmunosorbent assayAntibodiesAntigenCausative agentAntigenic regionsBorreliosisBurgdorferiComparable sensitivityHuman serumMigransSerum