2007
Borrelia burgdorferi basic membrane proteins A and B participate in the genesis of Lyme arthritis
Pal U, Wang P, Bao F, Yang X, Samanta S, Schoen R, Wormser GP, Schwartz I, Fikrig E. Borrelia burgdorferi basic membrane proteins A and B participate in the genesis of Lyme arthritis. Journal Of Experimental Medicine 2007, 205: 133-141. PMID: 18166585, PMCID: PMC2234379, DOI: 10.1084/jem.20070962.Peer-Reviewed Original ResearchConceptsLyme arthritisMouse jointsB. burgdorferi antigensBurgdorferi-infected miceSevere arthritisSpirochete numbersArthritisHost responseLyme diseaseAffinity-purified antibodiesBorrelia burgdorferiChain reactionMiceOriginal phenotypeBasic membrane proteinMutant spirochetesGene expressionJointsInflammationPathogenesisAntigenDiseaseB. burgdorferi gene expressionA Tick Antioxidant Facilitates the Lyme Disease Agent's Successful Migration from the Mammalian Host to the Arthropod Vector
Narasimhan S, Sukumaran B, Bozdogan U, Thomas V, Liang X, DePonte K, Marcantonio N, Koski RA, Anderson JF, Kantor F, Fikrig E. A Tick Antioxidant Facilitates the Lyme Disease Agent's Successful Migration from the Mammalian Host to the Arthropod Vector. Cell Host & Microbe 2007, 2: 7-18. PMID: 18005713, PMCID: PMC2699493, DOI: 10.1016/j.chom.2007.06.001.Peer-Reviewed Original ResearchConceptsMammalian hostsComplex feeding sitesLyme disease agent Borrelia burgdorferiSuccessful migrationMammalian responseTick Ixodes scapularisTick salivary glandsReactive oxygen speciesFeeding sitesArthropod vectorsTick proteinsBurgdorferi-infected miceOxygen speciesEfficient vectorCritical roleSpirochete acquisitionIxodes scapularisB. burgdorferiPathogensHostBorrelia burgdorferiI. scapularisInflammatory cellsImmune cellsSurvival advantage
1999
Selective Anti-Inflammatory Action of Interleukin-11 in Murine Lyme Disease: Arthritis Decreases while Carditis Persists
Anguita J, Barthold S, Samanta S, Ryan J, Fikrig E. Selective Anti-Inflammatory Action of Interleukin-11 in Murine Lyme Disease: Arthritis Decreases while Carditis Persists. The Journal Of Infectious Diseases 1999, 179: 734-737. PMID: 9952389, DOI: 10.1086/314613.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalAnti-Inflammatory AgentsArthritis, InfectiousFemaleHumansInflammationInterferon-gammaInterleukin-11Interleukin-12Interleukin-4Lyme DiseaseMiceMice, Inbred C3HMyocarditisNitric Oxide SynthaseNitric Oxide Synthase Type IIRecombinant ProteinsRNA, MessengerTranscription, GeneticConceptsMurine Lyme diseaseIL-11Potent anti-inflammatory propertiesInducible nitric oxide synthaseLyme diseaseMurine Lyme carditisAnti-inflammatory actionRole of interleukinAnti-inflammatory propertiesNitric oxide synthaseInnate immune responseB. burgdorferi-infected miceBurgdorferi-infected miceLyme carditisCardiac inflammationLyme arthritisIL-12Less arthritisIL-4Oxide synthaseBlocking antibodiesImmune responseControl animalsInterleukin-11Borrelia burgdorferiBorrelia burgdorferi erpT expression in the arthropod vector and murine host
Fikrig E, Chen M, Barthold S, Anguita J, Feng W, Telford S, Flavell R. Borrelia burgdorferi erpT expression in the arthropod vector and murine host. Molecular Microbiology 1999, 31: 281-290. PMID: 9987129, DOI: 10.1046/j.1365-2958.1999.01171.x.Peer-Reviewed Original ResearchConceptsRNA polymerase chain reactionPolymerase chain reactionB. burgdorferi-infected miceBurgdorferi-infected miceB. burgdorferiMurine infectionChain reactionMurine hostImmunization of miceExtracutaneous sitesDifferential antibodyPersistent infectionArthropod vectorsIndirect immunofluorescenceBSK II mediumMiceImmunofluorescence studiesInfectionBurgdorferiAntibodiesAntigenBorrelia burgdorferi genesSmall minorityMammalian hostsNorthern blot
1997
OspA antibodies inhibit the acquisition of Borrelia burgdorferi by Ixodes ticks
de Silva AM, Fish D, Burkot TR, Zhang Y, Fikrig E. OspA antibodies inhibit the acquisition of Borrelia burgdorferi by Ixodes ticks. Infection And Immunity 1997, 65: 3146-3150. PMID: 9234767, PMCID: PMC175444, DOI: 10.1128/iai.65.8.3146-3150.1997.Peer-Reviewed Original ResearchConceptsOspA antibodiesB. burgdorferiPassive antibody transferB. burgdorferi infectionIxodes ticksSurface protein ABurgdorferi-infected miceB. burgdorferi-infected miceLarval infection ratesOuter surface protein AAntibody transferAntibody responseBurgdorferi infectionInfection rateInfected ticksMiceInhibitory effectAntibodiesLarval infectionBorrelia burgdorferiBurgdorferiInfectionLarval ticksReservoir competenceSpirochetesTemporal pattern of Borrelia burgdorferi p21 expression in ticks and the mammalian host.
Das S, Barthold SW, Giles SS, Montgomery RR, Telford SR, Fikrig E. Temporal pattern of Borrelia burgdorferi p21 expression in ticks and the mammalian host. Journal Of Clinical Investigation 1997, 99: 987-995. PMID: 9062357, PMCID: PMC507907, DOI: 10.1172/jci119264.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, BacterialAntigens, BacterialAntigens, SurfaceArthritis, InfectiousBacterial Outer Membrane ProteinsBacterial ProteinsBacterial VaccinesBorrelia burgdorferi GroupElectrophoresis, Gel, Pulsed-FieldEnzyme-Linked Immunosorbent AssayFemaleFlagellinFluorescent Antibody Technique, IndirectGene Expression Regulation, BacterialHumansImmunizationImmunization, PassiveImmunoblottingIxodesLipoproteinsLyme DiseaseMiceMice, Inbred C3HPlasmidsPolymerase Chain ReactionRecombinant ProteinsRNA, MessengerTime FactorsConceptsInfected miceHumoral responseLate-stage Lyme diseaseMarkers of infectionCourse of diseaseMurine Lyme borreliosisB. burgdorferiB. burgdorferi infectionHuman humoral responseIxodes dammini ticksBurgdorferi-infected miceLyme arthritisActive immunizationMammalian hostsPassive transferBurgdorferi infectionC3H miceMurine infectionDay 14P21 antibodyP21 expressionLyme borreliosisLyme diseaseMiceInfection
1996
Characterization of a 30-kDa Borrelia burgdorferi substrate-binding protein homologue
Das S, Shraga D, Gannon C, Lam TT, Feng S, Brunet LR, Telford SR, Barthold SW, Flavell RA, Fikrig E. Characterization of a 30-kDa Borrelia burgdorferi substrate-binding protein homologue. Research In Microbiology 1996, 147: 739-751. PMID: 9296108, DOI: 10.1016/s0923-2508(97)85121-2.Peer-Reviewed Original ResearchConceptsSubstrate-binding proteinGram-negative bacteriaPeriplasmic substrate-binding proteinB. burgdorferiProtein homologueOligopeptide permeaseChromosomal genesConsiderable homologyP30 expressionB. burgdorferi-infected miceMolecular massSensu latoAmino acidsC3H/HeN miceLyme diseaseB. burgdorferi sensu latoBurgdorferi-infected miceSubset of patientsP30 geneMurine Lyme borreliosisGenesLate-stage infectionRegion 36Recombinant p30Anti-p30 serum