2008
A Leishmania Ortholog of Macrophage Migration Inhibitory Factor Modulates Host Macrophage Responses
Kamir D, Zierow S, Leng L, Cho Y, Diaz Y, Griffith J, McDonald C, Merk M, Mitchell RA, Trent J, Chen Y, Kwong YK, Xiong H, Vermeire J, Cappello M, McMahon-Pratt D, Walker J, Bernhagen J, Lolis E, Bucala R. A Leishmania Ortholog of Macrophage Migration Inhibitory Factor Modulates Host Macrophage Responses. The Journal Of Immunology 2008, 180: 8250-8261. PMID: 18523291, PMCID: PMC2668862, DOI: 10.4049/jimmunol.180.12.8250.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, Differentiation, B-LymphocyteApoptosis Regulatory ProteinsCell LineCells, CulturedCrystallography, X-RayHistocompatibility Antigens Class IIHumansIntramolecular OxidoreductasesLeishmania majorMacrophage Migration-Inhibitory FactorsMacrophages, PeritonealMiceMice, Inbred BALB CMice, Inbred C3HMice, KnockoutMolecular Sequence DataRecombinant ProteinsStructural Homology, ProteinConceptsMacrophage migration inhibitory factorCD74-dependent mannerMigration inhibitory factorImmune defense mechanismsHuman macrophage migration inhibitory factorSmall molecule antagonistsActivation-induced apoptosisHost macrophage responseMIF receptorMIF proteinImmune destructionObligate intracellular parasitesMAP kinase activationERK1/2 MAP kinase activationInhibitory factorMacrophage responseLeishmania majorIntracellular parasitesHigh-resolution X-ray crystal structuresSpecies-specific inhibitionMacrophagesSignificant structural homologyKinase activationDefense mechanismsMammalian counterparts
2000
Evidence for a recent mutation giving rise to a truncated copy of a cysteine proteinase gene in Leishmania pifanoi
Boukai L, McMahon-Pratt D, Traub-Cseko Y. Evidence for a recent mutation giving rise to a truncated copy of a cysteine proteinase gene in Leishmania pifanoi. Parasitology International 2000, 49: 301-307. PMID: 11077264, DOI: 10.1016/s1383-5769(00)00060-x.Peer-Reviewed Original ResearchThe Immunologically Protective P-4 Antigen ofLeishmania Amastigotes A DEVELOPMENTALLY REGULATED SINGLE STRAND-SPECIFIC NUCLEASE ASSOCIATED WITH THE ENDOPLASMIC RETICULUM*
Kar S, Soong L, Colmenares M, Goldsmith-Pestana K, McMahon-Pratt D. The Immunologically Protective P-4 Antigen ofLeishmania Amastigotes A DEVELOPMENTALLY REGULATED SINGLE STRAND-SPECIFIC NUCLEASE ASSOCIATED WITH THE ENDOPLASMIC RETICULUM*. Journal Of Biological Chemistry 2000, 275: 37789-37797. PMID: 10969068, DOI: 10.1074/jbc.m002149200.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulumSpecific nuclease activityBlot analysisImmunofluorescence microscopic analysisIntracellular amastigote stageProtein sequence dataSingle-strand nucleasePeripheral blood mononuclear cellsSouthern blot analysisNorthern blot analysisBlood mononuclear cellsMembrane proteinsRNA stabilitySubcellular localizationFuture vaccine studiesDNA repairSequence dataTerminal domainAmerican cutaneous leishmaniasisSequence analysisNuclease activityAmastigote stagePromastigote stageSpecific nucleasesT helper
1994
The biosynthesis, processing, and immunolocalization of Leishmania pifanoi amastigote cysteine proteinases
Duboise S, Vannier-Santos M, Costa-Pinto D, Rivas L, Pan A, Traub-Cseko Y, De Souza W, McMahon-Pratt D. The biosynthesis, processing, and immunolocalization of Leishmania pifanoi amastigote cysteine proteinases. Molecular And Biochemical Parasitology 1994, 68: 119-132. PMID: 7891736, DOI: 10.1016/0166-6851(94)00157-x.Peer-Reviewed Original ResearchConceptsTerminal domainMature proteinaseFlagellar pocketCysteine proteinasesParasite cell divisionCOOH-terminal domainProtein sequence dataAmastigote cysteine proteinasesElectron microscopic immunolocalizationLow-level secretionCell divisionSequence dataPlasma membraneIntracellular targetingLeishmania pifanoiProteolytic processingPulse labelingAcid proteinase inhibitorsProteinase inhibitorsCultured amastigotesLevel secretionL. pifanoiExtension domainBiosynthesisEnzymatic processing
1993
Identification of two distinct cysteine proteinase genes of Leishmania pifanoi axenic amastigotes using the polymerase chain reaction
Traub-Cseko Y, Duboise M, Boukai L, McMahon-Pratt D. Identification of two distinct cysteine proteinase genes of Leishmania pifanoi axenic amastigotes using the polymerase chain reaction. Molecular And Biochemical Parasitology 1993, 57: 101-115. PMID: 8426606, DOI: 10.1016/0166-6851(93)90248-v.Peer-Reviewed Original ResearchConceptsC-terminal extensionCysteine proteinase geneComparative sequence analysisCysteine proteinasesActive site residuesAmastigote cysteine proteinasesMexicana complexChromosome locationGenomic arrangementDistinct genesCDNA sequenceSequence dataDNA sequencesTrypanosoma bruceiProtein sequencesBiological functionsProteinase geneSite residuesDegenerate primersSelective conservationLysosomal organellesGlycine residueGenomic copiesSequence analysisLeishmania mexicana complex
1990
Molecular cloning and characterization of the immunologically protective surface glycoprotein GP46/M-2 of Leishmania amazonensis.
Lohman K, Langer P, McMahon-Pratt D. Molecular cloning and characterization of the immunologically protective surface glycoprotein GP46/M-2 of Leishmania amazonensis. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 8393-8397. PMID: 2236047, PMCID: PMC54962, DOI: 10.1073/pnas.87.21.8393.Peer-Reviewed Original ResearchConceptsGP46/MDNA sequence dataHydrophobic leader peptideCarboxyl-terminal domainMetal-binding motifGlycosyl-phosphatidylinositol anchorAmino-terminal portionProtozoan Leishmania amazonensisSouthern blot analysisGene familyMature proteinPutative attachment siteMolecular cloningProtein comprisesRepetitive sequencesSequence dataCarboxyl terminusCysteine residuesProtein sequencesLeader peptideMembrane locationBinding motifLeishmania amazonensisMembrane glycoproteinsBlot analysis