Comparison of test performance of a conventional PCR and two field-friendly tests to detect Coxiella burnetii DNA in ticks using Bayesian latent class analysis
Kamau M, Witte C, Goosen W, Mutinda M, Villinger J, Getange D, Khogali R, von Fricken M, Fèvre E, Zimmerman D, Linton Y, Miller M. Comparison of test performance of a conventional PCR and two field-friendly tests to detect Coxiella burnetii DNA in ticks using Bayesian latent class analysis. Frontiers In Veterinary Science 2024, 11: 1396714. PMID: 38962707, PMCID: PMC11220323, DOI: 10.3389/fvets.2024.1396714.Peer-Reviewed Original ResearchBayesian latent class analysisC. burnetii DNAEpidemiological roleHuman Q fever outbreakBayesian latent class analysis modelWild animalsDetect Coxiella burnetii DNADetect C. burnetii DNACoxiella burnetii DNAWild animal samplesPCR-HRMQ fever outbreakField-friendly techniquePositive ticksPCR high-resolution meltingTicksPCR-HRM assayZoonotic threatAnimal samplesWild animal habitatsQ feverHigh-resolution meltingMolecular techniquesCPCREstimate SECoxiella burnetii serostatus in dromedary camels (Camelus dromedarius) is associated with the presence of C. burnetii DNA in attached ticks in Laikipia County, Kenya
Rooney T, Fèvre E, Villinger J, Brenn‐White M, Cummings C, Chai D, Kamau J, Kiyong'a A, Getange D, Ochieng D, Kivali V, Zimmerman D, Rosenbaum M, Nutter F, Deem S. Coxiella burnetii serostatus in dromedary camels (Camelus dromedarius) is associated with the presence of C. burnetii DNA in attached ticks in Laikipia County, Kenya. Zoonoses And Public Health 2024, 71: 503-514. PMID: 38627945, DOI: 10.1111/zph.13127.Peer-Reviewed Original ResearchPCR-positive ticksCoxiella-like endosymbiontsTick poolsPresence of C. burnetii DNADromedary camelsCamelus dromedariusPrevalence of C. burnetiiEpidemiology of Q feverZoonotic diseaseIncreased total solidsQ feverAntibody test kitBacterium Coxiella burnetiiLivestock speciesTotal solidsTicksC. burnetiiReproductive lossesSubclinical infectionCamelsCoxiella burnetiiAge classesLivestockPublic health importanceDromedarius