2001
Unusual diversity in α-amanitin sensitivity of RNA polymerases in trichomonads
Vaňáčová Š, Tachezy J, Ullu E, Tschudi C. Unusual diversity in α-amanitin sensitivity of RNA polymerases in trichomonads. Molecular And Biochemical Parasitology 2001, 115: 239-247. PMID: 11420110, DOI: 10.1016/s0166-6851(01)00294-8.Peer-Reviewed Original ResearchConceptsUnusual diversityResistant RNA polymerase IIProtein coding genesParasitic protist Trichomonas vaginalisRNA polymerase IIAlpha-amanitin sensitivityProtist Trichomonas vaginalisGenus-specific patternsTritrichomonas foetusLysolecithin-permeabilized cellsUnicellular eukaryotesCoding genesPolymerase IIRNAP IIRNAP IIIRNA polymeraseTrichomonad speciesAlpha-amanitinT. vaginalisRNA synthesisΑ-amanitin sensitivityDiversityTrichomonadsRelated groupsEukaryotes
1997
Transcription of the Trypanosoma brucei spliced leader RNA gene is dependent only on the presence of upstream regulatory elements
Günzl A, Ullu E, Dörner M, Fragoso S, Hoffmann K, Milner J, Morita Y, Nguu E, Vanacova S, Wünsch S, Dare A, Kwon H, Tschudi C. Transcription of the Trypanosoma brucei spliced leader RNA gene is dependent only on the presence of upstream regulatory elements. Molecular And Biochemical Parasitology 1997, 85: 67-76. PMID: 9108549, DOI: 10.1016/s0166-6851(96)02816-2.Peer-Reviewed Original Research
1994
RNA polymerase III-mediated transcription of the trypanosome U2 small nuclear RNA gene is controlled by both intragenic and extragenic regulatory elements.
Fantoni A, Dare A, Tschudi C. RNA polymerase III-mediated transcription of the trypanosome U2 small nuclear RNA gene is controlled by both intragenic and extragenic regulatory elements. Molecular And Cellular Biology 1994, 14: 2021-2028. PMID: 8114733, PMCID: PMC358562, DOI: 10.1128/mcb.14.3.2021.Peer-Reviewed Original ResearchMeSH KeywordsAmanitinsAnimalsBase SequenceConsensus SequenceDNA, ProtozoanGenes, ProtozoanMolecular Sequence DataPromoter Regions, GeneticRegulatory Sequences, Nucleic AcidRNA Polymerase IIIRNA, Small NuclearSequence AlignmentSequence DeletionSequence Homology, Nucleic AcidTranscription, GeneticTrypanosomaConceptsU2 small nuclear RNA genesSmall nuclear RNA genesNuclear RNA genesU2 snRNA genesSnRNA genesRNA genesCis-acting regulatory sequencesRNA polymerase III transcriptsInsect form trypanosomesSnRNA gene expressionRNA polymerase IIInternal control regionRNA polymerase IIIPolymerase III transcriptsTranscription start siteIII-like enzymeTRNA genesPolymerase IIRNA polymeraseRegulatory sequencesU2 snRNAControl regionPolymerase IIIStart siteRegulatory elements
1990
Permeable trypanosome cells as a model system for transceiption and trans-splicing
Ullu E, Tschudi C. Permeable trypanosome cells as a model system for transceiption and trans-splicing. Nucleic Acids Research 1990, 18: 3319-3326. PMID: 2356121, PMCID: PMC330939, DOI: 10.1093/nar/18.11.3319.Peer-Reviewed Original ResearchConceptsAlpha-tubulin RNASL RNAFree linear moleculesHigh molecular weight RNAMolecular weight RNARNA speciesLeader RNATrypanosome cellsDifferent genesWeight RNARNATranscriptionModel systemCritical rolePermeable cellsConcentrations of monovalentIntronsCellsGenesLesser extentHigh potassium concentrationSpeciesTrypanosomaMgCl2 concentrationLinear molecules