2001
Unusual diversity in α-amanitin sensitivity of RNA polymerases in trichomonads
Vaňáčová Š, Tachezy J, Ullu E, Tschudi C. Unusual diversity in α-amanitin sensitivity of RNA polymerases in trichomonads. Molecular And Biochemical Parasitology 2001, 115: 239-247. PMID: 11420110, DOI: 10.1016/s0166-6851(01)00294-8.Peer-Reviewed Original ResearchConceptsUnusual diversityResistant RNA polymerase IIProtein coding genesParasitic protist Trichomonas vaginalisRNA polymerase IIAlpha-amanitin sensitivityProtist Trichomonas vaginalisGenus-specific patternsTritrichomonas foetusLysolecithin-permeabilized cellsUnicellular eukaryotesCoding genesPolymerase IIRNAP IIRNAP IIIRNA polymeraseTrichomonad speciesAlpha-amanitinT. vaginalisRNA synthesisΑ-amanitin sensitivityDiversityTrichomonadsRelated groupsEukaryotes
1995
Accurate Transcription of the Trypanosoma brucei U2 Small Nuclear RNA Gene in a Homologous Extract *
Günzl A, Tschudi C, Nakaar V, Ullu E. Accurate Transcription of the Trypanosoma brucei U2 Small Nuclear RNA Gene in a Homologous Extract *. Journal Of Biological Chemistry 1995, 270: 17287-17291. PMID: 7542235, DOI: 10.1074/jbc.270.29.17287.Peer-Reviewed Original ResearchConceptsIntragenic control regionRNA polymerase IIIControl regionPolymerase IIIU2 small nuclear RNA genesTranscription systemSmall nuclear RNA genesGene expressionInsect form trypanosomesNuclear RNA genesU2 snRNA genesSnRNA gene promotersB-box elementsFull transcriptional activitySeries of mutationsSnRNA genesRNA genesInsect formsRNA polymeraseGene promoterTrypanosoma bruceiCell-free extractsTransient expressionBox elementTranscriptional activityAn unusual liaison: Small nuclear and cytoplasmis RNA genes team up with tRNA genes in trypanosomatid protozoa
Nakaar V, Tschudi C, Ullu E. An unusual liaison: Small nuclear and cytoplasmis RNA genes team up with tRNA genes in trypanosomatid protozoa. Trends In Parasitology 1995, 11: 225-228. DOI: 10.1016/0169-4758(95)80085-9.Peer-Reviewed Original ResearchTrypanosomatid protozoaRNA polymerase III transcription unitSequence-specific DNA-binding proteinsCis-acting DNA sequencesBasal transcriptional apparatusDNA-binding proteinsTrans-acting factorsStudy of transcriptionTRNA genesTranscriptional apparatusTranscription unitPromoter structureAccessory factorsRNA polymeraseSmall nuclearDNA sequencesGene teamsCytoplasmic RNAGene expressionEnhancer elementsBiochemical interactionsTransfection technologyRecent insightsProtozoaDiverse set
1994
RNA polymerase III-mediated transcription of the trypanosome U2 small nuclear RNA gene is controlled by both intragenic and extragenic regulatory elements.
Fantoni A, Dare A, Tschudi C. RNA polymerase III-mediated transcription of the trypanosome U2 small nuclear RNA gene is controlled by both intragenic and extragenic regulatory elements. Molecular And Cellular Biology 1994, 14: 2021-2028. PMID: 8114733, PMCID: PMC358562, DOI: 10.1128/mcb.14.3.2021.Peer-Reviewed Original ResearchMeSH KeywordsAmanitinsAnimalsBase SequenceConsensus SequenceDNA, ProtozoanGenes, ProtozoanMolecular Sequence DataPromoter Regions, GeneticRegulatory Sequences, Nucleic AcidRNA Polymerase IIIRNA, Small NuclearSequence AlignmentSequence DeletionSequence Homology, Nucleic AcidTranscription, GeneticTrypanosomaConceptsU2 small nuclear RNA genesSmall nuclear RNA genesNuclear RNA genesU2 snRNA genesSnRNA genesRNA genesCis-acting regulatory sequencesRNA polymerase III transcriptsInsect form trypanosomesSnRNA gene expressionRNA polymerase IIInternal control regionRNA polymerase IIIPolymerase III transcriptsTranscription start siteIII-like enzymeTRNA genesPolymerase IIRNA polymeraseRegulatory sequencesU2 snRNAControl regionPolymerase IIIStart siteRegulatory elementsRNA Polymerase III-Mediated Transcription of the Trypanosome U2 Small Nuclear RNA Gene Is Controlled by both Intragenic and Extragenic Regulatory Elements
Fantoni A, Dare A, Tschudi C. RNA Polymerase III-Mediated Transcription of the Trypanosome U2 Small Nuclear RNA Gene Is Controlled by both Intragenic and Extragenic Regulatory Elements. Molecular And Cellular Biology 1994, 14: 2021-2028. DOI: 10.1128/mcb.14.3.2021-2028.1994.Peer-Reviewed Original ResearchU2 small nuclear RNA genesSmall nuclear RNA genesNuclear RNA genesU2 snRNA genesSnRNA genesRNA genesRNA polymeraseCis-acting regulatory sequencesRNA polymerase III transcriptsInsect form trypanosomesSnRNA gene expressionRNA polymerase IIInternal control regionPolymerase III transcriptsTranscription start siteIII-like enzymeTRNA genesPolymerase IIRegulatory sequencesU2 snRNAControl regionStart siteRegulatory elementsPromoter elementsMutant constructs