Noncoding Subgenomic Flavivirus RNA Is Processed by the Mosquito RNA Interference Machinery and Determines West Nile Virus Transmission by Culex pipiens Mosquitoes
Göertz GP, Fros JJ, Miesen P, Vogels CB, van der Bent ML, Geertsema C, Koenraadt CJ, van Rij RP, van Oers MM, Pijlman GP. Noncoding Subgenomic Flavivirus RNA Is Processed by the Mosquito RNA Interference Machinery and Determines West Nile Virus Transmission by Culex pipiens Mosquitoes. Journal Of Virology 2016, 90: 10145-10159. PMID: 27581979, PMCID: PMC5105652, DOI: 10.1128/jvi.00930-16.Peer-Reviewed Original ResearchConceptsSubgenomic flavivirus RNARNA interference machineryCulex pipiens mosquitoesWest Nile virusInterference machinerySfRNA productionPipiens mosquitoesBiological functionsUntranslated regionNile virusPivotal biological functionsIntrathoracic injectionWild-type WNVRNA deep sequencingKey biological functionsBlood mealFlavivirus RNAMosquito cell linesFlavivirus West Nile virusViral genomic RNAMosquito midgut barrierMutant West Nile virusTick-borne flavivirusesWNV-infected mosquitoesMammalian cells