2020
Reverse Transcription-Polymerase Chain Reaction Testing on Filter Paper-Dried Serum for Laboratory-Based Dengue Surveillance-American Samoa, 2018.
Curren E, Tufa A, Hancock W, Biggerstaff B, Vaifanua-Leo J, Montalbo C, Sharp T, Fischer M, Hills S, Gould C. Reverse Transcription-Polymerase Chain Reaction Testing on Filter Paper-Dried Serum for Laboratory-Based Dengue Surveillance-American Samoa, 2018. American Journal Of Tropical Medicine And Hygiene 2020, 102: 622-624. PMID: 31933466, PMCID: PMC7056409, DOI: 10.4269/ajtmh.19-0800.Peer-Reviewed Original ResearchConceptsReal-time reverse transcription-polymerase chain reactionResource-limited settingsTranscription polymerase chain reaction testingArboviral diseasesReverse transcription-polymerase chain reactionLaboratory-based surveillanceTranscription-polymerase chain reactionDengue virus RNADENV RNAReaction testingDiagnostic testingDengue surveillanceReference laboratorySerumSurveillance of dengueVirus RNAChain reactionDiseaseSurveillanceDengueTravel-Associated and Locally Acquired Dengue Cases — United States, 2010–2017
Rivera A, Adams L, Sharp T, Lehman J, Waterman S, Paz-Bailey G. Travel-Associated and Locally Acquired Dengue Cases — United States, 2010–2017. MMWR Morbidity And Mortality Weekly Report 2020, 69: 149-154. PMID: 32053577, PMCID: PMC7017959, DOI: 10.15585/mmwr.mm6906a1.Peer-Reviewed Original ResearchConceptsReverse transcription-polymerase chain reactionDengue casesDENV nonstructural protein 1 (NS1) antigenNonstructural protein 1 antigenMosquito-transmitted dengue virusAcute febrile illnessIgM positive casesAnti-DENV antibodiesTranscription-polymerase chain reactionProbable dengue casesCompetent mosquito vectorsFebrile illnessMedian ageDENV infectionFatal casesDENV antigenEpidemiologic trendsTravel-associatedDengue virusFamily FlaviviridaeVirus RNADistrict of ColumbiaChain reactionMosquito vectorsAntigen
2017
Persistence of Zika Virus in Body Fluids — Final Report
Paz-Bailey G, Rosenberg ES, Doyle K, Munoz-Jordan J, Santiago GA, Klein L, Perez-Padilla J, Medina FA, Waterman SH, Adams L, Lozier M, Bertrán-Pasarell J, Garcia Gubern C, Alvarado L, Sharp T. Persistence of Zika Virus in Body Fluids — Final Report. New England Journal Of Medicine 2017, 379: 1234-1243. PMID: 28195756, PMCID: PMC5831142, DOI: 10.1056/nejmoa1613108.Peer-Reviewed Original ResearchZIKV RNAZIKV RNA detectionVaginal secretionsIgM enzyme-linked immunosorbentDetectable ZIKV RNARNA detectionZika virus RNAEnzyme-linked immunosorbentZIKV exposureZIKV infectionWeek 4RNA clearanceZika virusSurveillance sitesFirst monthRT-PCRSerumVirus RNAMonthsBody fluidsUrineSecretionDaysMedianRegression models