Early Infant Human Immunodeficiency Virus Type 1 Detection Suitable for Resource-Limited Settings with Multiple Circulating Subtypes by Use of Nested Three-Monoplex DNA PCR and Dried Blood Spots▿
Zhang Q, Wang L, Jiang Y, Fang L, Pan P, Gong S, Yao J, Tang YW, Vermund SH, Jia Y. Early Infant Human Immunodeficiency Virus Type 1 Detection Suitable for Resource-Limited Settings with Multiple Circulating Subtypes by Use of Nested Three-Monoplex DNA PCR and Dried Blood Spots▿. Journal Of Clinical Microbiology 2007, 46: 721-726. PMID: 18077639, PMCID: PMC2238144, DOI: 10.1128/jcm.01539-07.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsBloodBlotting, WesternChinaDNA PrimersDNA, ViralEnv Gene Products, Human Immunodeficiency VirusGag Gene Products, Human Immunodeficiency VirusHIV AntibodiesHIV InfectionsHIV-1HumansInfantInfant, NewbornPol Gene Products, Human Immunodeficiency VirusPolymerase Chain ReactionReproducibility of ResultsSensitivity and SpecificitySpecimen HandlingConceptsHIV-1 subtypesMonths of ageDiverse HIV-1 subtypesEarly infant HIV-1 diagnosisHuman immunodeficiency virus type 1 (HIV-1) infectionHIV-1 DNA PCRVirus type 1 infectionDNA PCRType 1 infectionHIV-1 diagnosisDiverse HIV-1Resource limited settingsAntibody enzyme immunoassayBlood spot samplesInfected mothersMaternal antibodiesCRF01-AEDBS specimensEpidemiologic dataHIV-1Deceased infantsInfantsCRF07-BCMultiple subtypesBlood spots