Whole genome sequencing of Plasmodium falciparum from dried blood spots using selective whole genome amplification
Oyola S, Ariani C, Hamilton W, Kekre M, Amenga-Etego L, Ghansah A, Rutledge G, Redmond S, Manske M, Jyothi D, Jacob C, Otto T, Rockett K, Newbold C, Berriman M, Kwiatkowski D. Whole genome sequencing of Plasmodium falciparum from dried blood spots using selective whole genome amplification. Malaria Journal 2016, 15: 597. PMID: 27998271, PMCID: PMC5175302, DOI: 10.1186/s12936-016-1641-7.Peer-Reviewed Original ResearchMeSH KeywordsBloodDesiccationDNA PrimersDNA, ProtozoanGenome, ProtozoanHumansNucleic Acid Amplification TechniquesPlasmodium falciparumSequence Analysis, DNASpecimen HandlingConceptsVenous bloodSelective whole genome amplificationMalaria patientsDBS samplesBlood spotsPlasmodium falciparumClinical samplesSymptomatic malaria patientsHigh quality clinical samplesDried Blood SpotsP. falciparum DNAMalaria parasite Plasmodium falciparumHead comparisonParasite Plasmodium falciparumDrug resistance lociHealthcare applicationsPatientsWhole-genome sequencingGenome amplificationDBS extractsSNP concordanceWhole genome amplificationFalciparumCurrent dataScalable way