2014
CD4 T cell activation by B cells in human Leishmania (Viannia)infection
Rodriguez-Pinto D, Saravia NG, McMahon-Pratt D. CD4 T cell activation by B cells in human Leishmania (Viannia)infection. BMC Infectious Diseases 2014, 14: 108. PMID: 24568275, PMCID: PMC3937821, DOI: 10.1186/1471-2334-14-108.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedBiotinB-LymphocytesCD4-Positive T-LymphocytesColombiaFemaleFlow CytometryFluorescein-5-isothiocyanateGene Expression RegulationHumansImmunoglobulin MInterferon-gammaInterleukin-6Leishmania braziliensisLeishmaniasis, CutaneousLeukocytes, MononuclearLymphocyte ActivationMaleMiddle AgedOvalbuminTumor Necrosis Factor-alphaYoung AdultConceptsCD4 T cellsCutaneous leishmaniasis patientsT cellsB cellsLeishmaniasis patientsT cell activationLeishmania antigenImmune responseCell activationT-cell activation parametersSpecific CD4 T cellsEffective adaptive immune responseCD4 T cell activationB-cell activation markersCultures of PBMCUpregulation of CD86Cell activation markersCostimulatory molecule CD86Activation markers CD25Adaptive immune responsesHuman cutaneous leishmaniasisPurified B cellsT cell culturesHuman B cell linesHuman B cells
2007
Treponema pallidum Elicits Innate and Adaptive Cellular Immune Responses in Skin and Blood during Secondary Syphilis: A Flow-Cytometric Analysis
Salazar JC, Cruz AR, Pope CD, Valderrama L, Trujillo R, Saravia NG, Radolf JD. Treponema pallidum Elicits Innate and Adaptive Cellular Immune Responses in Skin and Blood during Secondary Syphilis: A Flow-Cytometric Analysis. The Journal Of Infectious Diseases 2007, 195: 879-887. PMID: 17299719, PMCID: PMC2131710, DOI: 10.1086/511822.Peer-Reviewed Original ResearchConceptsCellular immune responsesPeripheral bloodT cellsSecondary syphilisBlister fluidImmune responseControl subjectsDC-specific intercellular adhesion molecule 3DC-SIGNSystemic cellular immune responsesAdaptive cellular immune responsesEpidermal suction blistersIntercellular adhesion molecule 3Plasmacytoid dendritic cellsDermal T cellsHealthy control subjectsHuman leukocyte antigenActivation marker CD69Adaptive immune responsesMonocytes/macrophagesFlow cytometric analysisDermal leukocytesLeukocyte immunophenotypesHIV coreceptorsDendritic cells
2000
Distinct Innate and Acquired Immune Responses to Leishmania in Putative Susceptible and Resistant Human Populations Endemically Exposed to L. (Viannia) panamensis Infection
Bosque, Saravia, Valderrama, Milon. Distinct Innate and Acquired Immune Responses to Leishmania in Putative Susceptible and Resistant Human Populations Endemically Exposed to L. (Viannia) panamensis Infection. Scandinavian Journal Of Immunology 2000, 51: 533-541. PMID: 10792848, DOI: 10.1046/j.1365-3083.2000.00724.x.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAnimalsCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCells, CulturedDisease SusceptibilityEndemic DiseasesFemaleHumansHypersensitivity, DelayedImmunity, InnateInterferon-gammaInterleukin-10Leishmania guyanensisLeishmaniasis, MucocutaneousMacrophagesMaleMiddle AgedMonocytesRecombinant ProteinsRecurrenceRisk FactorsTritiumTumor Necrosis Factor-alphaConceptsPeripheral blood mononuclear cellsRecurrent diseaseL. panamensis infectionsCD8 T lymphocytesType hypersensitivity responseBlood mononuclear cellsAcquired Immune ResponsesTumor necrosis factorProduction of interferonEntry of parasitesLeishmania Viannia panamensisPanamensis infectionRecurrent leishmaniasisHypersensitivity responseImmunological basisSkin testMononuclear cellsNecrosis factorT lymphocytesImmune responseSubclinical infectionLinear regression analysisEvidence of differencesDistinct innateIntracellular amastigotes
1997
In Situ Characterization of the Human Host Response to Leishmania panamensis
Palma G, Saravia N. In Situ Characterization of the Human Host Response to Leishmania panamensis. American Journal Of Dermatopathology 1997, 19: 585-590. PMID: 9415615, DOI: 10.1097/00000372-199712000-00006.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAnimalsAntibodies, ProtozoanAntigen-Antibody ReactionsAntigens, ProtozoanBiomarkersBiopsyCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesEpidermisFluorescent Antibody Technique, IndirectGranulocytesGranulomaHumansImmunity, CellularImmunohistochemistryLeishmaniaLeishmaniasisLymphocyte ActivationMaleMiddle AgedNecrosisRegression AnalysisSkinConceptsHuman host responseHost responseLeishmania panamensisPresence of amastigotesT cell subpopulationsImmune response dataIndirect immunoperoxidase methodCD8 ratioDermal infiltrateMale patientsHistopathologic changesParticular cell populationPrognostic indicatorLeishmania infectionSkin biopsiesPlasma cellsT cellsDermal distributionCutaneous leishmaniasisProspective analysisB cellsB lymphocytesUpper dermisImmunoperoxidase methodDisease evolution