2015
Streptococcus pneumoniae biofilm formation and dispersion during colonization and disease
Chao Y, Marks LR, Pettigrew MM, Hakansson AP. Streptococcus pneumoniae biofilm formation and dispersion during colonization and disease. Frontiers In Cellular And Infection Microbiology 2015, 4: 194. PMID: 25629011, PMCID: PMC4292784, DOI: 10.3389/fcimb.2014.00194.Peer-Reviewed Original ResearchConceptsNasopharyngeal environmentConcomitant virus infectionPneumococcal biofilmsMiddle ear infectionRespiratory epithelial cellsMillions of infectionsPneumococcal biofilm formationPneumococcal diseaseEar infectionsSterile sitesInvasive diseasePneumococcal colonizationHigh prevalenceVirus infectionDisease processDistinct phenotypic propertiesMucosal surfacesStreptococcus pneumoniaeVaccine escapeHuman nasopharynxDiseaseEpithelial cellsInfectionPneumococciAntibiotic resistance
2014
Dynamic Changes in the Streptococcus pneumoniae Transcriptome during Transition from Biofilm Formation to Invasive Disease upon Influenza A Virus Infection
Pettigrew MM, Marks LR, Kong Y, Gent JF, Roche-Hakansson H, Hakansson AP. Dynamic Changes in the Streptococcus pneumoniae Transcriptome during Transition from Biofilm Formation to Invasive Disease upon Influenza A Virus Infection. Infection And Immunity 2014, 82: 4607-4619. PMID: 25135685, PMCID: PMC4249342, DOI: 10.1128/iai.02225-14.Peer-Reviewed Original ResearchConceptsWhole transcriptome shotgun sequencingPneumococcal transcriptomeTransparent colony phenotypeVirus infectionTranscriptional differencesShotgun sequencingUpregulated genesFebrile range temperaturesColony phenotypeInfluenza A Virus InfectionNutrient availabilityTranscriptomeS. pneumoniae pathogenesisA virus infectionProduction of bacteriocinsHost immune responsePlanktonic cellsBiofilm formationGenesPlanktonic bacteriaBiofilm bacteriaExtracellular ATPCarbohydrate metabolismBacteriaEnzymatic assayDetection of livestock-associated methicillin-resistant Staphylococcus aureus among swine workers in Romania
Huang E, Gurzau AE, Hanson BM, Kates AE, Smith TC, Pettigrew MM, Spinu M, Rabinowitz PM. Detection of livestock-associated methicillin-resistant Staphylococcus aureus among swine workers in Romania. Journal Of Infection And Public Health 2014, 7: 323-332. PMID: 24821273, DOI: 10.1016/j.jiph.2014.03.008.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgricultureAnimal HusbandryAnimalsCarrier StateFemaleHumansLivestockMaleMethicillin-Resistant Staphylococcus aureusMiddle AgedMolecular TypingNasal MucosaOccupational ExposureOropharynxPrevalenceRomaniaStaphylococcal InfectionsStaphylococcal Protein ASurveys and QuestionnairesSwineConceptsLivestock-associated methicillin-resistant Staphylococcus aureusMethicillin-resistant Staphylococcus aureusLA-MRSASwine workersLivestock workersSpa type t034Commercial pig farmsCommunity-associated MRSA strainsPig farmsDevastating pathogenStaphylococcus aureusFarmsCommercial movementsSubstantial health risksMRSA carriersMRSA colonizationHigh morbidityRisk factorsMRSA strainsHealth statusBiosafety practicesCommunity contactsProduction facilitiesPathogensEpidemiology
2012
Virulence of Streptococcus pneumoniae serotype 6C in experimental otitis media
Sabharwal V, Figueira M, Pelton SI, Pettigrew MM. Virulence of Streptococcus pneumoniae serotype 6C in experimental otitis media. Microbes And Infection 2012, 14: 712-718. PMID: 22414497, PMCID: PMC3382049, DOI: 10.1016/j.micinf.2012.02.008.Peer-Reviewed Original ResearchConceptsAcute otitis mediaExperimental otitis mediaPneumococcal conjugate vaccineOtitis mediaSerotype 6CInvasive pneumococcal diseaseOverall carriage rateLow-density infectionsMiddle ear infectionStreptococcus pneumoniae serotype 6CReplacement serotypesPneumococcal diseaseVaccine serotypesConjugate vaccineNonvaccine serotypesCarriage rateEar infectionsNasopharyngeal colonizationChinchilla modelStreptococcus pneumoniaeHost defenseSerotypesDisease potentialInfectionDisease
2011
Streptococcus pneumoniae Clonal Complex 199: Genetic Diversity and Tissue-Specific Virulence
Thomas JC, Figueira M, Fennie KP, Laufer AS, Kong Y, Pichichero ME, Pelton SI, Pettigrew MM. Streptococcus pneumoniae Clonal Complex 199: Genetic Diversity and Tissue-Specific Virulence. PLOS ONE 2011, 6: e18649. PMID: 21533186, PMCID: PMC3077395, DOI: 10.1371/journal.pone.0018649.Peer-Reviewed Original ResearchConceptsChinchilla modelHeptavalent pneumococcal conjugate vaccinePneumococcal conjugate vaccineSerotype 19A isolatesMiddle ear isolatesS. pneumoniae isolatesLong-term effectivenessConjugate vaccinePneumococcal diseaseOtitis mediaVaccinated populationReplacement diseaseBlood isolatesInvasive diseaseCarriage isolatesPneumoniae isolatesCerebrospinal fluidEar isolatesFuture vaccinesImportant causeStreptococcus pneumoniaeMiddle earDiseaseVirulence potentialVaccine
2010
Capacity of serotype 19A and 15B/C Streptococcus pneumoniae isolates for experimental otitis media: Implications for the conjugate vaccine
Laufer AS, Thomas JC, Figueira M, Gent JF, Pelton SI, Pettigrew MM. Capacity of serotype 19A and 15B/C Streptococcus pneumoniae isolates for experimental otitis media: Implications for the conjugate vaccine. Vaccine 2010, 28: 2450-2457. PMID: 20067753, PMCID: PMC2851619, DOI: 10.1016/j.vaccine.2009.12.078.Peer-Reviewed Original ResearchConceptsSerotype 19ASerotype 15B/CChinchilla otitis media modelMiddle ear infectionOtitis media modelDisease prevention effortsStreptococcus pneumoniae isolatesExperimental otitis mediaStreptococcus pneumoniae serotypesQuantitative PCRConjugate vaccineOtitis mediaEar infectionsPneumoniae isolatesVaccine formulationsPneumoniae serotypesSame sequence typeVaccine targetsPrevention effortsPathogenic potentialDifferent serotypesSequence typesSerotypesIsolatesVaccine
2000
Wolbachia neither induces nor suppresses transcripts encoding antimicrobial peptides
Bourtzis K, Pettigrew M, O’Neill S. Wolbachia neither induces nor suppresses transcripts encoding antimicrobial peptides. Insect Molecular Biology 2000, 9: 635-639. PMID: 11122472, DOI: 10.1046/j.1365-2583.2000.00224.x.Peer-Reviewed Original ResearchConceptsInsect speciesCytoplasmic incompatibilityDifferent tissue tropismsSingle WolbachiaDefensin genesAntibacterial genesArthropod speciesAnopheles gambiaeWolbachiaExpression patternsMosquito speciesSpeciesGenesMosquito defensinUbiquitous parasiteHost immune systemImmune systemTissue tropismAntimicrobial peptidesTranscriptionAedes aegyptiAedes albopictusReproductive abnormalitiesDrosophilaParthenogenesis
1999
Semliki Forest virus as an expression vector in insect cell lines
Pettigrew M, O'neill S. Semliki Forest virus as an expression vector in insect cell lines. Insect Molecular Biology 1999, 8: 409-414. PMID: 10469258, DOI: 10.1046/j.1365-2583.1999.83132.x.Peer-Reviewed Original ResearchConceptsInsect cell linesGreen fluorescent proteinCell linesExpression vectorSemliki Forest virus (SFV) expression vectorAedes albopictus cell lineVirus expression vectorNovel transcriptsGFP proteinGene constructsSemliki Forest virusNorthern analysisFluorescent proteinViral transcriptionInitial characterizationInfection levelsTranscriptsProteinTranscriptionLinesVirusC6/36
1997
In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line
O'Neill S, Pettigrew M, Sinkins S, Braig H, Andreadis T, Tesh R. In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line. Insect Molecular Biology 1997, 6: 33-39. PMID: 9013253, DOI: 10.1046/j.1365-2583.1997.00157.x.Peer-Reviewed Original Research