2009
Inhibition of Neutrophil Function by Two Tick Salivary Proteins
Guo X, Booth CJ, Paley MA, Wang X, DePonte K, Fikrig E, Narasimhan S, Montgomery RR. Inhibition of Neutrophil Function by Two Tick Salivary Proteins. Infection And Immunity 2009, 77: 2320-2329. PMID: 19332533, PMCID: PMC2687334, DOI: 10.1128/iai.01507-08.Peer-Reviewed Original ResearchConceptsPolymorphonuclear leukocytesPMN functionNumber of PMNPMN integrinsPMN adherenceNeutrophil functionSpirochete burdenTick salivary proteinsTick salivaLyme diseaseTick attachmentSalivary glandsBorrelia burgdorferiTick feedingCausative agentReduced levelsInhibitory proteinSalivaBlood mealAntihemostatic activityInfectionInhibitionSalivary proteinsHematophagous arthropodsTick Ixodes scapularis
2006
Human Neutrophil Calprotectin Reduces the Susceptibility of Borrelia burgdorferi to Penicillin
Montgomery RR, Schreck K, Wang X, Malawista SE. Human Neutrophil Calprotectin Reduces the Susceptibility of Borrelia burgdorferi to Penicillin. Infection And Immunity 2006, 74: 2468-2472. PMID: 16552081, PMCID: PMC1418918, DOI: 10.1128/iai.74.4.2468-2472.2006.Peer-Reviewed Original ResearchConceptsClearance of spirochetesB. burgdorferiSites of inflammationTherapeutic dosesCalprotectinGrowth of spirochetesLeukocyte componentsNeutrophil calprotectinAntibiotic sensitivitySpirochetal agentLyme diseaseTherapeutic antibioticsBorrelia burgdorferiPenicillin GProtein calprotectinInflammationBurgdorferiSpirochetesDiseaseDoxycyclineDosesClearance
2004
TROSPA, an Ixodes scapularis Receptor for Borrelia burgdorferi
Pal U, Li X, Wang T, Montgomery RR, Ramamoorthi N, deSilva AM, Bao F, Yang X, Pypaert M, Pradhan D, Kantor FS, Telford S, Anderson JF, Fikrig E. TROSPA, an Ixodes scapularis Receptor for Borrelia burgdorferi. Cell 2004, 119: 457-468. PMID: 15537536, DOI: 10.1016/j.cell.2004.10.027.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, BacterialAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesBase SequenceBorrelia burgdorferiCloning, MolecularGene Expression RegulationHost-Parasite InteractionsIntestinesIxodesLipoproteinsMiceMice, Inbred C3HMolecular Sequence DataReceptors, Cell SurfaceRecombinant ProteinsConceptsLyme disease agent Borrelia burgdorferiSurvival of spirochetesTick receptorMammalian hostsRNA interferenceEfficient colonizationTROSPAOuter surface protein ABorrelia burgdorferiPathogen adherencePathogen transmissionProtein AB. burgdorferi outer surface protein AMRNA levelsIxodes scapularisB. burgdorferiColonizationSurface protein AReceptorsArthropodsBurgdorferiMammalsRepressionSpirochetesTicksMyeloid Differentiation Antigen 88 Deficiency Impairs Pathogen Clearance but Does Not Alter Inflammation in Borrelia burgdorferi-Infected Mice
Liu N, Montgomery RR, Barthold SW, Bockenstedt LK. Myeloid Differentiation Antigen 88 Deficiency Impairs Pathogen Clearance but Does Not Alter Inflammation in Borrelia burgdorferi-Infected Mice. Infection And Immunity 2004, 72: 3195-3203. PMID: 15155621, PMCID: PMC415708, DOI: 10.1128/iai.72.6.3195-3203.2004.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsAntibodies, BacterialAntigens, DifferentiationArthritisBorrelia burgdorferiDNA, BacterialInflammationLyme DiseaseMacrophages, PeritonealMiceMice, Inbred C57BLMice, KnockoutMyeloid Differentiation Factor 88MyocarditisOpsonin ProteinsPhagocytosisReceptors, ImmunologicUrineConceptsToll-like receptor 2Days of infectionPathogen burdenWT miceAcute inflammationB. burgdorferi-specific antibodyPathogen-specific adaptive immunityMyD88-dependent signaling pathwaysTumor necrosis factor alphaBurgdorferi-specific antibodiesImmunoglobulin G1 responsesTLR2-deficient miceInnate immune cellsBorrelia burgdorferiNecrosis factor alphaWild-type miceIgM titersImmune cellsInflammatory responseFactor alphaAdaptive immunitySpirochete Borrelia burgdorferiWT macrophagesReceptor 2Pathogen clearanceTick Saliva Reduces Adherence and Area of Human Neutrophils
Montgomery RR, Lusitani D, de Boisfleury Chevance A, Malawista SE. Tick Saliva Reduces Adherence and Area of Human Neutrophils. Infection And Immunity 2004, 72: 2989-2994. PMID: 15102811, PMCID: PMC387908, DOI: 10.1128/iai.72.5.2989-2994.2004.Peer-Reviewed Original ResearchDisruption of Ixodes scapularis anticoagulation by using RNA interference
Narasimhan S, Montgomery RR, DePonte K, Tschudi C, Marcantonio N, Anderson JF, Sauer JR, Cappello M, Kantor FS, Fikrig E. Disruption of Ixodes scapularis anticoagulation by using RNA interference. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 1141-1146. PMID: 14745044, PMCID: PMC337020, DOI: 10.1073/pnas.0307669100.Peer-Reviewed Original ResearchConceptsAnti-factor Xa activityNumerous infectious diseasesIxodes scapularis ticksRNA interferenceMultiple anticoagulantsReduction of mRNAXa activityVaccine candidatesInfectious diseasesTick salivaAnticoagulantsBabesia microtiPhysiologic functionI. scapularis salivaSalivary glandsBorrelia burgdorferiTick feedingScapularis ticksAnticoagulant activityAnaplasma phagocytophilumTick engorgementSuch interventionsEngorgement weightEngorgementKey target
2003
Calprotectin, an Abundant Cytosolic Protein from Human Polymorphonuclear Leukocytes, Inhibits the Growth of Borrelia burgdorferi
Lusitani D, Malawista SE, Montgomery RR. Calprotectin, an Abundant Cytosolic Protein from Human Polymorphonuclear Leukocytes, Inhibits the Growth of Borrelia burgdorferi. Infection And Immunity 2003, 71: 4711-4716. PMID: 12874352, PMCID: PMC166021, DOI: 10.1128/iai.71.8.4711-4716.2003.Peer-Reviewed Original ResearchConceptsPolymorphonuclear leukocytesB. burgdorferiGranule-poor cytoplastsBorrelia burgdorferiBlood polymorphonuclear leukocytesHuman blood polymorphonuclear leukocytesNumerous polymorphonuclear leukocytesPotent bacteriostatic agentHuman polymorphonuclear leukocytesU-CytAcute inflammationCalprotectinModulatory roleLyme spirocheteLyme diseaseSpecific antibodiesProtein calprotectinBurgdorferiLeukocytesAbundant cytosolic proteinOxidative mechanismsBacteriostatic agentRegrowth assaysQuantitative microscopicInflammation
2002
Human phagocytic cells in the early innate immune response to Borrelia burgdorferi
Montgomery RR, Lusitani D, de Boisfleury Chevance A, Malawista SE. Human phagocytic cells in the early innate immune response to Borrelia burgdorferi. The Journal Of Infectious Diseases 2002, 185: 1773-1779. PMID: 12085324, DOI: 10.1086/340826.Peer-Reviewed Original ResearchConceptsPolymorphonuclear leukocytesImmune responseEarly innate immune responseKilling of spirochetesSpecific antibodiesBorrelia burgdorferiSecondary immune responseInnate immune responseInnate immune systemHuman phagocytic cellsSpirochete clearanceMononuclear cellsImmune systemLyme diseasePhagocytic cellsNatural infectionMature macrophagesSpirochetesIntracellular colocalizationAntibodiesBurgdorferiFirst cellsLimited uptakeCellsMonocytesBorrelia burgdorferi Are Susceptible to Killing by a Variety of Human Polymorphonuclear Leukocyte Components
Lusitani D, Malawista SE, Montgomery RR. Borrelia burgdorferi Are Susceptible to Killing by a Variety of Human Polymorphonuclear Leukocyte Components. The Journal Of Infectious Diseases 2002, 185: 797-804. PMID: 11920297, DOI: 10.1086/339341.Peer-Reviewed Original ResearchConceptsIntact polymorphonuclear leukocytesPolymorphonuclear leukocytesB. burgdorferiKilling of BorreliaHuman neutrophil peptides 1Neutrophil peptide-1Bactericidal/permeability-increasing proteinIntact human polymorphonuclear leukocytesHuman polymorphonuclear leukocytesPermeability-increasing proteinProteinase 3Peptide-1Leukocyte componentsNitric oxideLL-37Specific antibodiesBorrelia burgdorferiBurgdorferiGranule contentsEfficient killingSpirochetesCritical roleKillingLeukocytes
2000
Geographic clustering of an outer surface protein A mutant of Borrelia burgdorferi. Possible implications of multiple variants for Lyme disease persistence
Malawista SE, Montgomery RR, Wang X, Fu LL, Giles SS. Geographic clustering of an outer surface protein A mutant of Borrelia burgdorferi. Possible implications of multiple variants for Lyme disease persistence. Rheumatology 2000, 39: 537-541. PMID: 10852986, DOI: 10.1093/rheumatology/39.5.537.Peer-Reviewed Original ResearchConceptsLyme arthritisIndex patientsDisease persistenceChronic Lyme arthritisJoint fluid samplesSurface protein AOuter surface protein ANovel epitopesImmune systemPatientsOuter surface proteinsWestern blotLyme diseasePatients' jointsMixed infectionsBorrelia burgdorferiFluid samplesSerumArthritisSurface proteinsGeographic clusteringOspAProtein AFusion protein
1997
Borrelia burgdorferi strain-specific Osp C-mediated immunity in mice
Bockenstedt LK, Hodzic E, Feng S, Bourrel KW, de Silva A, Montgomery RR, Fikrig E, Radolf JD, Barthold SW. Borrelia burgdorferi strain-specific Osp C-mediated immunity in mice. Infection And Immunity 1997, 65: 4661-4667. PMID: 9353047, PMCID: PMC175668, DOI: 10.1128/iai.65.11.4661-4667.1997.Peer-Reviewed Original ResearchConceptsChallenge infectionC antiserumImmunization studiesStrain-specific immunityAntibody-mediated immunityPassive immunization studiesImmune mouse serumSurface protein AOuter surface protein ADisease regressionInfected miceBorreliacidal activityC antibodySpirochete Borrelia burgdorferiAnimal modelsImmune serumMouse serumInfectionLyme borreliosisMiceHyperimmune serumOsp AImmunofluorescence studiesBorrelia burgdorferiSurface expression
1994
Fc- And Non-Fc-Mediated Phagocytosis Of Borrelia Burgdorferi By Maerophages
Montgomery R, Nathanson M, Malawista S. Fc- And Non-Fc-Mediated Phagocytosis Of Borrelia Burgdorferi By Maerophages. The Journal Of Infectious Diseases 1994, 170: 890-893. PMID: 7930732, DOI: 10.1093/infdis/170.4.890.Peer-Reviewed Original ResearchConceptsConfocal fluorescence microscopyLyme disease spirocheteB. burgdorferiFluorescence microscopyBacterial surface antigensFc receptorsSurface antigenMacrophagesBorrelia burgdorferiSpirochetesBurgdorferiMajor roleSimilar lackComparable efficiencyUptakeColocalizationPhagocytosisInfectionAntigenLocalizationOuter surface proteins E and F of Borrelia burgdorferi, the agent of Lyme disease
Lam TT, Nguyen TP, Montgomery RR, Kantor FS, Fikrig E, Flavell RA. Outer surface proteins E and F of Borrelia burgdorferi, the agent of Lyme disease. Infection And Immunity 1994, 62: 290-298. PMID: 8262642, PMCID: PMC186099, DOI: 10.1128/iai.62.1.290-298.1994.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAntigens, BacterialBacterial Outer Membrane ProteinsBacterial ProteinsBase SequenceBlotting, WesternBorrelia burgdorferi GroupCloning, MolecularCodonFluorescent Antibody TechniqueGenes, BacterialHumansLipoproteinsLyme DiseaseMolecular Sequence DataMolecular WeightOperonRegulatory Sequences, Nucleic AcidRestriction MappingSequence AlignmentSequence Homology, Nucleic AcidSolubilityConceptsOspE genesMolecular massSignal peptidase IIConsensus cleavage sequenceTranscriptional unitsLeader sequenceCommon promoterBp downstreamOuter surface proteinsProtein EStop codonSurface lipoproteinsLabeling showBorrelia burgdorferiGenesHydrophobic domainCleavage sequenceSurface proteinsAmino acidsPeptidase IIProteinOuter surface protein EGel electrophoresisNucleotidesImmunofluorescence studiesBorrelia burgdorferi and the macrophage: Routine annihilation but occasional haven?
Montgomery RR, Malawista SE. Borrelia burgdorferi and the macrophage: Routine annihilation but occasional haven? Trends In Parasitology 1994, 10: 154-157. PMID: 15275485, DOI: 10.1016/0169-4758(94)90268-2.Peer-Reviewed Original Research
1993
The fate of Borrelia burgdorferi, the agent for Lyme disease, in mouse macrophages. Destruction, survival, recovery.
Montgomery RR, Nathanson MH, Malawista SE. The fate of Borrelia burgdorferi, the agent for Lyme disease, in mouse macrophages. Destruction, survival, recovery. The Journal Of Immunology 1993, 150: 909-15. PMID: 8423346, DOI: 10.4049/jimmunol.150.3.909.Peer-Reviewed Original ResearchConceptsUptake of spirochetesConfocal fluorescence microscopyLikely candidate siteEndocytic pathwayB. burgdorferiBorrelia burgdorferiFluorescence microscopyIntracellular persistenceLyme diseaseInfected cellsPersistence of spirochetesMouse macrophagesIntracellular organismsPositive compartmentsPossible pathogenetic mechanismsExtracellular organismsOrganismsAcridine orangeCellsMultiple time pointsPathogenetic mechanismsSpirochetesInfectious agentsCompartmentsLyme spirochete