2023
Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages
Oliveira G, Vogels C, Zolfaghari A, Saraf S, Klitting R, Weger-Lucarelli J, P Leon K, Ontiveros C, Agarwal R, Tsetsarkin K, Harris E, Ebel G, Wohl S, Grubaugh N, Andersen K. Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages. PLOS Neglected Tropical Diseases 2023, 17: e0011055. PMID: 36753510, PMCID: PMC9907835, DOI: 10.1371/journal.pntd.0011055.Peer-Reviewed Original ResearchConceptsHuman primary cellsFitness differencesVirus lineagesRapid molecular evolutionPrimary cellsShort generation timeAmino acid sitesFitness changesHigh mutation ratePhenotypic evolutionMolecular evolutionPositive selectionMutation rateLineagesPhenotypic analysisPhenotypic changesRNA virusesGeneration timeRecombinant virusesAedes aegypti mosquitoesReplicative fitnessFitnessAegypti mosquitoesMosquitoesZika virus
2021
Reproducing the Rift Valley fever virus mosquito-lamb-mosquito transmission cycle
Wichgers Schreur PJ, Vloet RPM, Kant J, van Keulen L, Gonzales JL, Visser TM, Koenraadt CJM, Vogels CBF, Kortekaas J. Reproducing the Rift Valley fever virus mosquito-lamb-mosquito transmission cycle. Scientific Reports 2021, 11: 1477. PMID: 33446733, PMCID: PMC7809480, DOI: 10.1038/s41598-020-79267-1.Peer-Reviewed Original ResearchConceptsRift Valley fever virusAnimal modelsFeeding of mosquitoesNatural target speciesMortality of newbornsEfficacy of vaccinesHigher infection rateViremia levelsIntravenous inoculationTransmission of mosquitoVector competence experimentsAedes aegypti mosquitoesBlood-feeding behaviorRVFV infectionInfected mosquitoesExtrinsic incubation periodInfection rateMembrane feedingFever virusRVFV transmissionAbortion stormsAegypti mosquitoesBlood mealVirusTransmission cycle
2020
Impact of Gut Bacteria on the Infection and Transmission of Pathogenic Arboviruses by Biting Midges and Mosquitoes
Möhlmann TWR, Vogels CBF, Göertz GP, Pijlman GP, ter Braak CJF, te Beest DE, Hendriks M, Nijhuis EH, Warris S, Drolet BS, van Overbeek L, Koenraadt CJM. Impact of Gut Bacteria on the Infection and Transmission of Pathogenic Arboviruses by Biting Midges and Mosquitoes. Microbial Ecology 2020, 80: 703-717. PMID: 32462391, PMCID: PMC7476999, DOI: 10.1007/s00248-020-01517-6.Peer-Reviewed Original ResearchConceptsAntibiotic treatmentGut bacteriaChikungunya virusInfection ratePathogenic arbovirusesInfectious blood mealAegypti mosquitoesGut bacterial communitiesResident gut bacteriaGut bacterial compositionSchmallenberg virusAedes aegypti mosquitoesArbovirus infectionViral pathogensVirusTreatmentBlood mealInfectionMidgut bacteriaArbovirus transmissionHealth of animalsMosquitoesArbovirusesZikaBacterial compositionThe invasive Asian bush mosquito Aedes japonicus found in the Netherlands can experimentally transmit Zika virus and Usutu virus
Abbo SR, Visser TM, Wang H, Göertz GP, Fros JJ, Abma-Henkens MHC, Geertsema C, Vogels CBF, Koopmans MPG, Reusken CBEM, Hall-Mendelin S, Hall RA, van Oers MM, Koenraadt CJM, Pijlman GP. The invasive Asian bush mosquito Aedes japonicus found in the Netherlands can experimentally transmit Zika virus and Usutu virus. PLOS Neglected Tropical Diseases 2020, 14: e0008217. PMID: 32282830, PMCID: PMC7153878, DOI: 10.1371/journal.pntd.0008217.Peer-Reviewed Original ResearchConceptsZika virusUsutu virusFed mosquitoesMidgut barrierMosquito midgut barrierGuillain-Barré syndromeInfectious blood mealSevere neurological impairmentSevere congenital microcephalyField-collected AeWest Nile virusClinical manifestationsMild diseaseUSUV infectionNeurological impairmentCongenital microcephalyRNA responseVirus disseminationArboviral diseasesNile virusVirus transmissionDroplet feedingVirusFemale mosquitoesBlood mealA satellite repeat-derived piRNA controls embryonic development of Aedes
Halbach R, Miesen P, Joosten J, Taşköprü E, Rondeel I, Pennings B, Vogels C, Merkling S, Koenraadt C, Lambrechts L, van Rij R. A satellite repeat-derived piRNA controls embryonic development of Aedes. Nature 2020, 580: 274-277. PMID: 32269344, PMCID: PMC7145458, DOI: 10.1038/s41586-020-2159-2.Peer-Reviewed Original ResearchConceptsPIWI-interacting RNAsSatellite repeatsEmbryonic developmentGene expressionAbundant piwi-interacting RNAsLocal chromatin structureGlobal gene expressionTandem repeat elementsSequence-specific geneMosquito Aedes aegyptiPiRNA productionEukaryotic chromosomesChromatin structureEuchromatic regionsPiRNA sequencesMosquito biologyRepeat elementsDevelopmental arrestRepeatsDiverse classAedes aegyptiGenesTranscriptsCentral functionExpression
2019
Subgenomic flavivirus RNA binds the mosquito DEAD/H-box helicase ME31B and determines Zika virus transmission by Aedes aegypti
Göertz GP, van Bree JWM, Hiralal A, Fernhout BM, Steffens C, Boeren S, Visser TM, Vogels CBF, Abbo SR, Fros JJ, Koenraadt CJM, van Oers MM, Pijlman GP. Subgenomic flavivirus RNA binds the mosquito DEAD/H-box helicase ME31B and determines Zika virus transmission by Aedes aegypti. Proceedings Of The National Academy Of Sciences Of The United States Of America 2019, 116: 19136-19144. PMID: 31488709, PMCID: PMC6754610, DOI: 10.1073/pnas.1905617116.Peer-Reviewed Original ResearchConceptsSubgenomic flavivirus RNAInfectious blood mealZika virusMosquito infectionWild-type Zika virusesFlavivirus RNAMosquito midgut barrierArthropod-borne flavivirusZika virus transmissionBlood mealGlobal human health threatMosquito cell culturesZIKV infectionMosquito salivaIntrathoracic injectionViral Small Interfering RNAsInfected mosquitoesViral titersAntiviral activityFlavivirus replicationFlavivirus transmissionMidgut barrierHealth threatInfectionInfected cellsTravel Surveillance and Genomics Uncover a Hidden Zika Outbreak during the Waning Epidemic
Grubaugh ND, Saraf S, Gangavarapu K, Watts A, Tan AL, Oidtman RJ, Ladner JT, Oliveira G, Matteson NL, Kraemer MUG, Vogels CBF, Hentoff A, Bhatia D, Stanek D, Scott B, Landis V, Stryker I, Cone MR, Kopp EW, Cannons AC, Heberlein-Larson L, White S, Gillis LD, Ricciardi MJ, Kwal J, Lichtenberger PK, Magnani DM, Watkins DI, Palacios G, Hamer DH, Network G, Gardner LM, Perkins TA, Baele G, Khan K, Morrison A, Isern S, Michael SF, Andersen KG. Travel Surveillance and Genomics Uncover a Hidden Zika Outbreak during the Waning Epidemic. Cell 2019, 178: 1057-1071.e11. PMID: 31442400, PMCID: PMC6716374, DOI: 10.1016/j.cell.2019.07.018.Peer-Reviewed Original ResearchArbovirus coinfection and co-transmission: A neglected public health concern?
Vogels CBF, Rückert C, Cavany SM, Perkins TA, Ebel GD, Grubaugh ND. Arbovirus coinfection and co-transmission: A neglected public health concern? PLOS Biology 2019, 17: e3000130. PMID: 30668574, PMCID: PMC6358106, DOI: 10.1371/journal.pbio.3000130.Peer-Reviewed Original ResearchConceptsImpact of coinfectionPublic health concernPublic healthEpidemiological synergyVirus coinfectionAedes aegypti mosquitoesClinical diseaseSuch coinfectionsZika virusCoinfectionOutbreaks of virusesHealth concernAegypti mosquitoesMultiple virusesTransmission dynamicsVirusHealthHumansMosquitoesDiseaseDengue
2018
Vector competence of biting midges and mosquitoes for Shuni virus
Möhlmann TWR, Oymans J, Schreur P, Koenraadt CJM, Kortekaas J, Vogels CBF. Vector competence of biting midges and mosquitoes for Shuni virus. PLOS Neglected Tropical Diseases 2018, 12: e0006993. PMID: 30532189, PMCID: PMC6285265, DOI: 10.1371/journal.pntd.0006993.Peer-Reviewed Original ResearchConceptsShuni virusInfection rateInfectious blood mealEvidence of transmissionHigher infection rateFebrile childrenCongenital malformationsNeurological diseasesPotential transmissionPotential involvementHigh pathogenicityZoonotic potentialSimbu serogroupBlood mealVector competenceVirusMosquitoesRiskBroad tropismArthropod vectorsAedes aegyptiAnimalsCulex pipiens pipiensFurther researchFuture epizooticsConserved motifs in the hypervariable domain of chikungunya virus nsP3 required for transmission by Aedes aegypti mosquitoes
Göertz GP, Lingemann M, Geertsema C, Abma-Henkens MHC, Vogels CBF, Koenraadt CJM, van Oers MM, Pijlman GP. Conserved motifs in the hypervariable domain of chikungunya virus nsP3 required for transmission by Aedes aegypti mosquitoes. PLOS Neglected Tropical Diseases 2018, 12: e0006958. PMID: 30412583, PMCID: PMC6249005, DOI: 10.1371/journal.pntd.0006958.Peer-Reviewed Original ResearchConceptsCHIKV replicationChikungunya virusAedes aegypti mosquitoesMosquito cellsAegypti mosquitoesTransmission of CHIKVCHIKV infectionHypervariable domainMosquito salivaHost proteinsInfectionCHIKV mutantsChikungunya Virus nsP3Non-structural proteinsCHIKV nsP3Arthropod-borneEfficient infectionIntervention strategiesViral RNATransmission cycleMosquitoesFGDF motifsCellsMammalian cellsRich motif
2017
Mosquito co-infection with Zika and chikungunya virus allows simultaneous transmission without affecting vector competence of Aedes aegypti
Göertz GP, Vogels CBF, Geertsema C, Koenraadt CJM, Pijlman GP. Mosquito co-infection with Zika and chikungunya virus allows simultaneous transmission without affecting vector competence of Aedes aegypti. PLOS Neglected Tropical Diseases 2017, 11: e0005654. PMID: 28570693, PMCID: PMC5469501, DOI: 10.1371/journal.pntd.0005654.Peer-Reviewed Original ResearchConceptsChikungunya virusZika virusAegypti mosquitoesIntrathoracic injectionPresence of ZIKVVector competenceInfectious blood mealSerious health burdenDual-color immunofluorescenceBlood mealMultiple mosquito bitesArthropod-borne virusZIKV productionSalivary gland barrierCase reportAedes aegypti mosquitoesHealth burdenOral exposureMosquito bitesViral titersPositive mosquitoesMosquito cellsBiteProfound barrierVirus