2021
Reproducing the Rift Valley fever virus mosquito-lamb-mosquito transmission cycle
Wichgers Schreur PJ, Vloet RPM, Kant J, van Keulen L, Gonzales JL, Visser TM, Koenraadt CJM, Vogels CBF, Kortekaas J. Reproducing the Rift Valley fever virus mosquito-lamb-mosquito transmission cycle. Scientific Reports 2021, 11: 1477. PMID: 33446733, PMCID: PMC7809480, DOI: 10.1038/s41598-020-79267-1.Peer-Reviewed Original ResearchConceptsRift Valley fever virusAnimal modelsFeeding of mosquitoesNatural target speciesMortality of newbornsEfficacy of vaccinesHigher infection rateViremia levelsIntravenous inoculationTransmission of mosquitoVector competence experimentsAedes aegypti mosquitoesBlood-feeding behaviorRVFV infectionInfected mosquitoesExtrinsic incubation periodInfection rateMembrane feedingFever virusRVFV transmissionAbortion stormsAegypti mosquitoesBlood mealVirusTransmission cycle
2020
Forced Zika Virus Infection of Culex pipiens Leads to Limited Virus Accumulation in Mosquito Saliva
Abbo SR, Vogels CBF, Visser TM, Geertsema C, van Oers MM, Koenraadt CJM, Pijlman GP. Forced Zika Virus Infection of Culex pipiens Leads to Limited Virus Accumulation in Mosquito Saliva. Viruses 2020, 12: 659. PMID: 32575394, PMCID: PMC7354520, DOI: 10.3390/v12060659.Peer-Reviewed Original ResearchConceptsZika virusGuillain-Barré syndromeZika virus infectionInfectious blood mealUrban transmission cyclesMosquito-borne pathogenMosquito salivaZIKV transmissionOral exposureVirus infectionCongenital microcephalySevere diseaseIntrathoracic injectionLarge outbreakVirus disseminationUsutu virusMidgut barrierBarrier functionMosquito midgutVirus transmissionSalivaBlood mealYellow fever mosquitoVector competenceVirusImpact of Gut Bacteria on the Infection and Transmission of Pathogenic Arboviruses by Biting Midges and Mosquitoes
Möhlmann TWR, Vogels CBF, Göertz GP, Pijlman GP, ter Braak CJF, te Beest DE, Hendriks M, Nijhuis EH, Warris S, Drolet BS, van Overbeek L, Koenraadt CJM. Impact of Gut Bacteria on the Infection and Transmission of Pathogenic Arboviruses by Biting Midges and Mosquitoes. Microbial Ecology 2020, 80: 703-717. PMID: 32462391, PMCID: PMC7476999, DOI: 10.1007/s00248-020-01517-6.Peer-Reviewed Original ResearchConceptsAntibiotic treatmentGut bacteriaChikungunya virusInfection ratePathogenic arbovirusesInfectious blood mealAegypti mosquitoesGut bacterial communitiesResident gut bacteriaGut bacterial compositionSchmallenberg virusAedes aegypti mosquitoesArbovirus infectionViral pathogensVirusTreatmentBlood mealInfectionMidgut bacteriaArbovirus transmissionHealth of animalsMosquitoesArbovirusesZikaBacterial compositionThe invasive Asian bush mosquito Aedes japonicus found in the Netherlands can experimentally transmit Zika virus and Usutu virus
Abbo SR, Visser TM, Wang H, Göertz GP, Fros JJ, Abma-Henkens MHC, Geertsema C, Vogels CBF, Koopmans MPG, Reusken CBEM, Hall-Mendelin S, Hall RA, van Oers MM, Koenraadt CJM, Pijlman GP. The invasive Asian bush mosquito Aedes japonicus found in the Netherlands can experimentally transmit Zika virus and Usutu virus. PLOS Neglected Tropical Diseases 2020, 14: e0008217. PMID: 32282830, PMCID: PMC7153878, DOI: 10.1371/journal.pntd.0008217.Peer-Reviewed Original ResearchConceptsZika virusUsutu virusFed mosquitoesMidgut barrierMosquito midgut barrierGuillain-Barré syndromeInfectious blood mealSevere neurological impairmentSevere congenital microcephalyField-collected AeWest Nile virusClinical manifestationsMild diseaseUSUV infectionNeurological impairmentCongenital microcephalyRNA responseVirus disseminationArboviral diseasesNile virusVirus transmissionDroplet feedingVirusFemale mosquitoesBlood meal
2019
Subgenomic flavivirus RNA binds the mosquito DEAD/H-box helicase ME31B and determines Zika virus transmission by Aedes aegypti
Göertz GP, van Bree JWM, Hiralal A, Fernhout BM, Steffens C, Boeren S, Visser TM, Vogels CBF, Abbo SR, Fros JJ, Koenraadt CJM, van Oers MM, Pijlman GP. Subgenomic flavivirus RNA binds the mosquito DEAD/H-box helicase ME31B and determines Zika virus transmission by Aedes aegypti. Proceedings Of The National Academy Of Sciences Of The United States Of America 2019, 116: 19136-19144. PMID: 31488709, PMCID: PMC6754610, DOI: 10.1073/pnas.1905617116.Peer-Reviewed Original ResearchConceptsSubgenomic flavivirus RNAInfectious blood mealZika virusMosquito infectionWild-type Zika virusesFlavivirus RNAMosquito midgut barrierArthropod-borne flavivirusZika virus transmissionBlood mealGlobal human health threatMosquito cell culturesZIKV infectionMosquito salivaIntrathoracic injectionViral Small Interfering RNAsInfected mosquitoesViral titersAntiviral activityFlavivirus replicationFlavivirus transmissionMidgut barrierHealth threatInfectionInfected cells
2018
Vector competence of biting midges and mosquitoes for Shuni virus
Möhlmann TWR, Oymans J, Schreur P, Koenraadt CJM, Kortekaas J, Vogels CBF. Vector competence of biting midges and mosquitoes for Shuni virus. PLOS Neglected Tropical Diseases 2018, 12: e0006993. PMID: 30532189, PMCID: PMC6285265, DOI: 10.1371/journal.pntd.0006993.Peer-Reviewed Original ResearchConceptsShuni virusInfection rateInfectious blood mealEvidence of transmissionHigher infection rateFebrile childrenCongenital malformationsNeurological diseasesPotential transmissionPotential involvementHigh pathogenicityZoonotic potentialSimbu serogroupBlood mealVector competenceVirusMosquitoesRiskBroad tropismArthropod vectorsAedes aegyptiAnimalsCulex pipiens pipiensFurther researchFuture epizootics
2017
Mosquito co-infection with Zika and chikungunya virus allows simultaneous transmission without affecting vector competence of Aedes aegypti
Göertz GP, Vogels CBF, Geertsema C, Koenraadt CJM, Pijlman GP. Mosquito co-infection with Zika and chikungunya virus allows simultaneous transmission without affecting vector competence of Aedes aegypti. PLOS Neglected Tropical Diseases 2017, 11: e0005654. PMID: 28570693, PMCID: PMC5469501, DOI: 10.1371/journal.pntd.0005654.Peer-Reviewed Original ResearchConceptsChikungunya virusZika virusAegypti mosquitoesIntrathoracic injectionPresence of ZIKVVector competenceInfectious blood mealSerious health burdenDual-color immunofluorescenceBlood mealMultiple mosquito bitesArthropod-borne virusZIKV productionSalivary gland barrierCase reportAedes aegypti mosquitoesHealth burdenOral exposureMosquito bitesViral titersPositive mosquitoesMosquito cellsBiteProfound barrierVirus
2016
Noncoding Subgenomic Flavivirus RNA Is Processed by the Mosquito RNA Interference Machinery and Determines West Nile Virus Transmission by Culex pipiens Mosquitoes
Göertz GP, Fros JJ, Miesen P, Vogels CB, van der Bent ML, Geertsema C, Koenraadt CJ, van Rij RP, van Oers MM, Pijlman GP. Noncoding Subgenomic Flavivirus RNA Is Processed by the Mosquito RNA Interference Machinery and Determines West Nile Virus Transmission by Culex pipiens Mosquitoes. Journal Of Virology 2016, 90: 10145-10159. PMID: 27581979, PMCID: PMC5105652, DOI: 10.1128/jvi.00930-16.Peer-Reviewed Original ResearchConceptsSubgenomic flavivirus RNARNA interference machineryCulex pipiens mosquitoesWest Nile virusInterference machinerySfRNA productionPipiens mosquitoesBiological functionsUntranslated regionNile virusPivotal biological functionsIntrathoracic injectionWild-type WNVRNA deep sequencingKey biological functionsBlood mealFlavivirus RNAMosquito cell linesFlavivirus West Nile virusViral genomic RNAMosquito midgut barrierMutant West Nile virusTick-borne flavivirusesWNV-infected mosquitoesMammalian cellsVector competence of northern European Culex pipiens biotypes and hybrids for West Nile virus is differentially affected by temperature
Vogels CB, Fros JJ, Göertz GP, Pijlman GP, Koenraadt CJ. Vector competence of northern European Culex pipiens biotypes and hybrids for West Nile virus is differentially affected by temperature. Parasites & Vectors 2016, 9: 393. PMID: 27388451, PMCID: PMC4937539, DOI: 10.1186/s13071-016-1677-0.Peer-Reviewed Original ResearchConceptsWest Nile virusVector competence studiesVector competenceMosquito midgut barrierNile virusInfectious blood mealCompetence studiesPipiens biotypesCulex pipiens biotypesViral titersSaliva samplesMidgut barrierInfectionBiotype pipiensBlood mealWNV presenceTitersOverall transmission rateVirusMosquitoesPipiens mosquitoesBiotype levelRisk assessmentPipiensMethodsWe
2015
West Nile Virus: High Transmission Rate in North-Western European Mosquitoes Indicates Its Epidemic Potential and Warrants Increased Surveillance
Fros JJ, Geertsema C, Vogels CB, Roosjen PP, Failloux AB, Vlak JM, Koenraadt CJ, Takken W, Pijlman GP. West Nile Virus: High Transmission Rate in North-Western European Mosquitoes Indicates Its Epidemic Potential and Warrants Increased Surveillance. PLOS Neglected Tropical Diseases 2015, 9: e0003956. PMID: 26225555, PMCID: PMC4520649, DOI: 10.1371/journal.pntd.0003956.Peer-Reviewed Original ResearchConceptsWest Nile virusEpidemic potentialLineage 1 West Nile VirusInfectious blood mealFurther epidemic spreadLineage 2 West Nile virusNeuroinvasive diseaseIntrathoracic injectionEuropean mosquitoesPathogenic flavivirusesInfection rateVirus activityLarge outbreakVirus disseminationWNV infection ratesNile virusWNV activityWNV lineagesBlood mealVector competenceInfectionCulex pipiens mosquitoesWNV transmissionEpidemic spreadDisease-free regions