2019
Developmental Expression of the Cytosolic Sulfotransferases in Human Liver
Dubaisi S, Caruso J, Gaedigk R, Vyhlidal C, Smith P, Hines R, Kocarek T, Runge-Morris M. Developmental Expression of the Cytosolic Sulfotransferases in Human Liver. Drug Metabolism And Disposition 2019, 47: dmd.119.086363. PMID: 30885913, PMCID: PMC6505379, DOI: 10.1124/dmd.119.086363.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultCytosolFemaleHumansInfantInfant, NewbornLiverMaleMiddle AgedRNA, MessengerSulfotransferasesYoung AdultConceptsMRNA levelsLiver specimensHuman liverReverse transcription-quantitative polymerase chain reactionTranscription-quantitative polymerase chain reactionProtein levelsRT-qPCR analysisHuman liver cytosolHuman liver samplesQuantitative polymerase chain reactionCytosolic sulfotransferasesRNA sequencingHepatic sulfotransferasesPolymerase chain reactionDrug eliminationPredominant organInfant liverLiverLiver samplesChain reactionLiver cytosolForeign chemicalsImportant metabolic roleInfantsAdditional findings
2015
Oxidative stress-responsive transcription factor NRF2 is not indispensable for the human hepatic Flavin-containing monooxygenase-3 (FMO3) gene expression in HepG2 cells
Rudraiah S, Gu X, Hines R, Manautou J. Oxidative stress-responsive transcription factor NRF2 is not indispensable for the human hepatic Flavin-containing monooxygenase-3 (FMO3) gene expression in HepG2 cells. Toxicology In Vitro 2015, 31: 54-59. PMID: 26616280, PMCID: PMC4695222, DOI: 10.1016/j.tiv.2015.11.016.Peer-Reviewed Original ResearchConceptsGene expressionFlavin-containing monooxygenasesStress-responsive transcription factor Nrf2Stress transcription factorsCytosolic regulatory proteinsHepG2 cellsPromoter-luciferase reporter constructsNrf2 target gene expressionGene regulation studiesCo-transfection studiesTarget gene expressionReporter gene activityHeme oxygenase-1Transcription factor Nrf2Luciferase reporter constructsTranscriptional regulationGene regulationKelch-like ECHGene activityTranscription factorsRegulatory proteinsRegulatory pathwaysReporter constructsExpression vectorRegulation studies
2012
Hepatobiliary Disposition of 17-OHPC and Taurocholate in Fetal Human Hepatocytes: A Comparison with Adult Human Hepatocytes
Sharma S, Ellis E, Gramignoli R, Dorko K, Tahan V, Hansel M, Mattison D, Caritis S, Hines R, Venkataramanan R, Strom S. Hepatobiliary Disposition of 17-OHPC and Taurocholate in Fetal Human Hepatocytes: A Comparison with Adult Human Hepatocytes. Drug Metabolism And Disposition 2012, 41: 296-304. PMID: 23129211, PMCID: PMC3558857, DOI: 10.1124/dmd.112.044891.Peer-Reviewed Original ResearchMeSH Keywords17 alpha-Hydroxyprogesterone CaproateAdultAge FactorsAgedBiological TransportCells, CulturedCold TemperatureCyclosporineFemaleGestational AgeHepatocytesHumansHydroxyprogesteronesKineticsMaleMembrane Transport ProteinsMiddle AgedMultidrug Resistance-Associated Protein 2RifampinRNA, MessengerTaurocholic AcidVerapamilYoung AdultConceptsHuman hepatocytesFetal human hepatocytesFetal human liverConcentration-dependent inhibitionAdult human hepatocytesBile acid transporterFetal circulationPlacental barrierRecurrent miscarriageSpontaneous abortionProgesterone metabolitesTaurocholate effluxAdult hepatocytesTherapeutic levelsLower mRNA levelsHepatobiliary dispositionHepatic transportersActivity of transportersActive efflux mechanismHuman liverHuman fetalAdverse effectsRole of transportersEfflux mechanismMRNA levels
2000
Ethnic Differences in Human Flavin-Containing Monooxygenase 2 (FMO2) Polymorphisms: Detection of Expressed Protein in African-Americans
Whetstine J, Yueh M, Hopp K, McCarver D, Williams D, Park C, Kang J, Cha Y, Dolphin C, Shephard E, Phillips I, Hines R. Ethnic Differences in Human Flavin-Containing Monooxygenase 2 (FMO2) Polymorphisms: Detection of Expressed Protein in African-Americans. Toxicology And Applied Pharmacology 2000, 168: 216-224. PMID: 11042094, DOI: 10.1006/taap.2000.9050.Peer-Reviewed Original ResearchRegulation of DHP receptor expression by elements in the 5′-flanking sequence
Liu L, Fan Q, El-Zaru M, Vanderpool K, Hines R, Marsh J. Regulation of DHP receptor expression by elements in the 5′-flanking sequence. AJP Heart And Circulatory Physiology 2000, 278: h1153-h1162. PMID: 10749709, DOI: 10.1152/ajpheart.2000.278.4.h1153.Peer-Reviewed Original ResearchMeSH Keywords5' Untranslated Regions8-Bromo Cyclic Adenosine MonophosphateAdrenergic alpha-AgonistsAdrenergic beta-AgonistsAnimalsBase SequenceCalcium Channels, L-TypeCells, CulturedElectrophoresisGene ExpressionGenes, ReporterGenetic Complementation TestGonadal Steroid HormonesIn Vitro TechniquesIsoproterenolLuciferasesMolecular Sequence DataMuscle Fibers, SkeletalMuscle, Smooth, VascularMyocardiumPhenylephrinePromoter Regions, GeneticRatsRNA, MessengerTestosteroneTranscription, GeneticTransfectionConceptsNeonatal rat cardiac ventricular myocytesVascular smooth muscle cellsRat cardiac ventricular myocytesSmooth muscle cellsCardiac ventricular myocytesCardiac-specific transcription factor Nkx2.5Receptor expressionResponse elementTranscription factor Nkx2.5Hormone responsivenessVentricular myocytesMuscle cellsSubunit gene promoterModulating activityHormone response elementsCAMP response elementPromoter-luciferase reporter gene constructsGene promoterTransient transfection experimentsRatsFusion geneSubunit geneAP-1 siteDevelopmental regulation of the L-type calcium channel α1C subunit expression in heart
Leu L, O'Hara D, Cala S, Poornima I, Hines R, Marsh J. Developmental regulation of the L-type calcium channel α1C subunit expression in heart. Molecular And Cellular Biochemistry 2000, 205: 101-109. PMID: 10821427, DOI: 10.1023/a:1007013900827.Peer-Reviewed Original ResearchConceptsRNase protection assaysProtection assaysNorthern analysisΑ1C subunitSize of proteinsAlternative splicingAbundance of mRNADevelopmental regulationSmaller transcriptTranscript sizeNative proteinKD proteinMolecular massDevelopmental stagesProteinSubunitsAdult ventriclesTranscriptsPostnatal developmentAbundanceExpressionSubunit expressionCalcium channel propertiesChannel propertiesReceptor message
1999
Flavin‐containing monooxygenase isoform 2: Developmental expression in fetal and neonatal rabbit lung
Larsen‐Su S, Krueger S, Yueh M, Lee M, Shehin S, Hines R, Williams D. Flavin‐containing monooxygenase isoform 2: Developmental expression in fetal and neonatal rabbit lung. Journal Of Biochemical And Molecular Toxicology 1999, 13: 187-193. PMID: 10098904, DOI: 10.1002/(sici)1099-0461(1999)13:3/4<187::aid-jbt9>3.0.co;2-6.Peer-Reviewed Original ResearchConceptsEarly developmental appearanceRabbit lungsNeonatal rabbit lungConstitutive cytochrome P450Developmental appearanceNeonatal lungToxic insultsLungMonooxygenase isoformsNumerous xenobioticsForeign chemicalsMonooxygenase functionCytochrome P450Major isoformsTissue-specific fashionDevelopmental expressionMammalian flavinIsoform 2Expression patternsIsoformsFMO2Individual isoformsNeonatesFetuses
1996
Identification of Multiple Rabbit Flavin-Containing Monooxygenase Form 1 (FMO1) Gene Promoters and Observation of Tissue-Specific DNase I Hypersensitive Sites
Luo Z, Hines R. Identification of Multiple Rabbit Flavin-Containing Monooxygenase Form 1 (FMO1) Gene Promoters and Observation of Tissue-Specific DNase I Hypersensitive Sites. Archives Of Biochemistry And Biophysics 1996, 336: 251-260. PMID: 8954572, DOI: 10.1006/abbi.1996.0555.Peer-Reviewed Original Research
1995
Developmental regulation of flavin-containing monooxygenase (FMO) isoforms 1 and 2 in pregnant rabbit
Lee M, Smiley S, Kadkhodayan S, Hines R, Williams D. Developmental regulation of flavin-containing monooxygenase (FMO) isoforms 1 and 2 in pregnant rabbit. Chemico-Biological Interactions 1995, 96: 75-85. PMID: 7720106, DOI: 10.1016/0009-2797(94)03584-u.Peer-Reviewed Original ResearchConceptsCortisol levelsRabbit lungsClassical cytochrome P450 inducersPeaks of progesteroneCytochrome P450 inducersPlasma cortisol levelsMaternal kidneyPregnant rabbitsPlasma progesteroneP450 inducersProgesteroneLungMRNA levelsHormonal regulationGestationMacrolide antibioticsTime courseKidneyFMO activityLiverAdministrationFMO2Mammalian flavinDiverse xenobioticsNumber of laboratoriesTissue-specific expression of flavin-containing monooxygenase (FMO) forms 1 and 2 in the rabbit.
Shehin-Johnson S, Williams D, Larsen-Su S, Stresser D, Hines R. Tissue-specific expression of flavin-containing monooxygenase (FMO) forms 1 and 2 in the rabbit. Journal Of Pharmacology And Experimental Therapeutics 1995, 272: 1293-9. PMID: 7891346.Peer-Reviewed Original Research
1993
Expression of human cytochrome P450 1A1 in DNA repair deficient and proficient human fibroblasts stably transformed with an inducible expression vector
States J, Quan T, Hines R, Novak R, Runge-Morris M. Expression of human cytochrome P450 1A1 in DNA repair deficient and proficient human fibroblasts stably transformed with an inducible expression vector. Carcinogenesis 1993, 14: 1643-1649. PMID: 8353849, DOI: 10.1093/carcin/14.8.1643.Peer-Reviewed Original ResearchMeSH Keywords7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxideAnimalsBase SequenceBenzo(a)pyreneBenzoflavonesBiotransformationCadmiumCadmium CompoundsChimeraCytochrome P-450 Enzyme SystemDNA RepairEnzyme InductionFibroblastsGene Expression Regulation, EnzymologicGenetic VectorsHumansIsoenzymesMiceMolecular Sequence DataPlasmidsRNA, MessengerSulfatesTransfectionTransformation, GeneticXeroderma PigmentosumConceptsCytochrome P450 1A1P450 1A1Inducible CYP1A1Inducible mouse metallothionein-I promoterLow dosesHuman skin fibroblastsProficient cellsPotent mutagenic agentCYP1A1 expressionCYP1A1 activityHuman cytochrome P450 1A1DNA repairCYP1A1Mouse metallothionein-I promoterCytotoxic effectsMetallothionein-I promoterSkin fibroblastsDifferential cytotoxicityDNA repair-proficient cellsToxic effectsMore cytotoxicCytochrome P450Normal cellsRepairHuman CYP1A1
1990
Expression of CYP1A1 Gene in Patients With Lung Cancer: Evidence for Cigarette Smoke-Induced Gene Expression in Normal Lung Tissue and for Altered Gene Regulation in Primary Pulmonary Carcinomas
McLemore T, Adelberg S, Liu M, McMahon N, Yu S, Hubbard W, Czerwinski M, Wood T, Storeng R, Lubet R, Eggleston J, Boyd M, Hines R. Expression of CYP1A1 Gene in Patients With Lung Cancer: Evidence for Cigarette Smoke-Induced Gene Expression in Normal Lung Tissue and for Altered Gene Regulation in Primary Pulmonary Carcinomas. Journal Of The National Cancer Institute 1990, 82: 1333-1339. PMID: 2380990, DOI: 10.1093/jnci/82.16.1333.Peer-Reviewed Original ResearchConceptsNormal lung tissuesLung tissueLung cancerFormer smokersCigarette smokersCYP1A1 gene expressionCYP1A1 geneActive cigarette smokersPrimary pulmonary carcinomasHuman pulmonary tissueMessenger RNA expressionPulmonary carcinogenesisCigarette smokingPulmonary carcinomaTime-dependent decreasePulmonary tissueGene expressionSmokersCarcinoma tissuesPatientsCancerTumorsRNA expressionMRNA levelsCYP1A1 mRNA
1989
Altered Regulation of the Cytochrome P4501A1 Gene: Novel Inducer-Independent Gene Expression in Pulmonary Carcinoma Cell Lines
McLemore T, Adelberg S, Czerwinski M, Hubbard W, Yu S, Storeng R, Wood T, Hines R, Boyd M. Altered Regulation of the Cytochrome P4501A1 Gene: Novel Inducer-Independent Gene Expression in Pulmonary Carcinoma Cell Lines. Journal Of The National Cancer Institute 1989, 81: 1787-1794. PMID: 2555530, DOI: 10.1093/jnci/81.23.1787.Peer-Reviewed Original ResearchConceptsPulmonary carcinoma cell linesLung cancer cell linesCarcinoma cell linesCancer cell linesLung cancerCell linesCYP1A1 gene expressionNon-small cell linesSmall cell lung carcinomaHuman lung cancer cell linesHeavy cigarette smokersHuman pulmonary carcinoma cell linesNon-small cell lung carcinoma cell lineSmall cell lung cancer linesNon-small cellCell lung carcinomaNormal human lungCell lung cancer linesHuman lung cancerCYP1A1 mRNACell lung carcinoma cell lineLung cancer cellsAryl hydrocarbon hydroxylase activityCYP1A1 geneLung carcinoma cell lineGlucocorticoid regulation of the rat cytochrome P450c (P450IA1) gene: Receptor binding within intron I
Mathis J, Houser W, Bresnick E, Cidlowski J, Hines R, Prough R, Simpson E. Glucocorticoid regulation of the rat cytochrome P450c (P450IA1) gene: Receptor binding within intron I. Archives Of Biochemistry And Biophysics 1989, 269: 93-105. PMID: 2916850, DOI: 10.1016/0003-9861(89)90090-8.Peer-Reviewed Original ResearchConceptsGlucocorticoid receptorConcentrations of dexamethasoneUntreated cellsMRNA contentFetal rat hepatocytesHalf-maximal increaseAddition of dexamethasonePresence of dexamethasoneCultured fetal rat hepatocytesGlucocorticoid-responsive elementRat hepatocytesDexamethasoneCytochrome P450cGlucocorticoid regulationHepG2 hepatoma cellsGlucocorticoidsAcetyltransferase activityHepatic cytochromeChloramphenicol acetyltransferase activityReceptorsNorthern blot analysisBlot analysisHepatoma cellsReceptor fraction
1987
Structural details of the human cytochrome P-450c gene
Iversen P, Heiger W, Bresnick E, Hines R. Structural details of the human cytochrome P-450c gene. Archives Of Biochemistry And Biophysics 1987, 256: 397-401. PMID: 3606130, DOI: 10.1016/0003-9861(87)90460-7.Peer-Reviewed Original ResearchAflatoxin B1-4-hydroxylase is associated with cytochrome P3-450 in C57BL6 mouse liver
Koser P, Faletto M, Bansal S, Caballes L, Bresnick E, Hines R, Gurtoo H. Aflatoxin B1-4-hydroxylase is associated with cytochrome P3-450 in C57BL6 mouse liver. Biochemical And Biophysical Research Communications 1987, 142: 872-878. PMID: 3103616, DOI: 10.1016/0006-291x(87)91494-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAroclorsAryl Hydrocarbon HydroxylasesBenzoflavonesBeta-NaphthoflavoneChlorodiphenyl (54% Chlorine)Cytochrome P-450 Enzyme SystemDNAEnzyme InductionFemaleLiverMethylcholanthreneMiceMice, Inbred C57BLMixed Function OxygenasesNucleic Acid HybridizationPolychlorinated BiphenylsRNA, Messenger
1986
Regulation of cytochrome P-450c by glucocorticoids and polycyclic aromatic hydrocarbons in cultured fetal rat hepatocytes
Mathis J, Prough R, Hines R, Bresnick E, Simpson E. Regulation of cytochrome P-450c by glucocorticoids and polycyclic aromatic hydrocarbons in cultured fetal rat hepatocytes. Archives Of Biochemistry And Biophysics 1986, 246: 439-448. PMID: 2870681, DOI: 10.1016/0003-9861(86)90490-x.Peer-Reviewed Original Research
1985
3-Methylcholanthrene-induced expression of the cytochrome P-450c gene
Foldes R, Hines R, Ho K, Shen M, Nagel K, Bresnick E. 3-Methylcholanthrene-induced expression of the cytochrome P-450c gene. Archives Of Biochemistry And Biophysics 1985, 239: 137-146. PMID: 4004253, DOI: 10.1016/0003-9861(85)90820-3.Peer-Reviewed Original ResearchConceptsNuclear RNA speciesCytochrome P-450c mRNARNA speciesCytochrome P-450c geneP-450c geneClone 46Nuclear transcription systemNuclear RNA precursorsNuclear RNA transportRat serum albumin geneDot hybridization experimentsSerum albumin geneNorthern blot analysisTranscriptional controlRNA transportRNA precursorsNuclear RNARegulation of inductionHybridization experimentsTranscription systemM13 subclonesAlbumin geneCytochrome P-450cRNA inductionSpecies
1984
Induction of cytochrome P450 by xenobiotics.
Bresnick E, Foldes R, Hines R. Induction of cytochrome P450 by xenobiotics. Pharmacological Reviews 1984, 36: 43s-51s. PMID: 6433366.Peer-Reviewed Original Research
1983
Molecular induction by phenobarbital of a rat hepatic form of cytochrome P-450: Expression of a 4-kilobase messenger RNA
Omiecinski C, Hines R, Foldes R, Levy J, Bresnick E. Molecular induction by phenobarbital of a rat hepatic form of cytochrome P-450: Expression of a 4-kilobase messenger RNA. Archives Of Biochemistry And Biophysics 1983, 227: 478-493. PMID: 6141764, DOI: 10.1016/0003-9861(83)90478-2.Peer-Reviewed Original Research