2015
Oxidative stress-responsive transcription factor NRF2 is not indispensable for the human hepatic Flavin-containing monooxygenase-3 (FMO3) gene expression in HepG2 cells
Rudraiah S, Gu X, Hines R, Manautou J. Oxidative stress-responsive transcription factor NRF2 is not indispensable for the human hepatic Flavin-containing monooxygenase-3 (FMO3) gene expression in HepG2 cells. Toxicology In Vitro 2015, 31: 54-59. PMID: 26616280, PMCID: PMC4695222, DOI: 10.1016/j.tiv.2015.11.016.Peer-Reviewed Original ResearchConceptsGene expressionFlavin-containing monooxygenasesStress-responsive transcription factor Nrf2Stress transcription factorsCytosolic regulatory proteinsHepG2 cellsPromoter-luciferase reporter constructsNrf2 target gene expressionGene regulation studiesCo-transfection studiesTarget gene expressionReporter gene activityHeme oxygenase-1Transcription factor Nrf2Luciferase reporter constructsTranscriptional regulationGene regulationKelch-like ECHGene activityTranscription factorsRegulatory proteinsRegulatory pathwaysReporter constructsExpression vectorRegulation studies
2007
Regulation of CYP3A4 Expression by PAR Transcription Factors
Mantei K, Le M, Klick D, Vyhlidal C, Hines R. Regulation of CYP3A4 Expression by PAR Transcription Factors. The FASEB Journal 2007, 21: a1183-a1183. DOI: 10.1096/fasebj.21.6.a1183-a.Peer-Reviewed Original ResearchD-element binding proteinCYP3A4 expressionHepatic leukemia factorCYP3A4 promoter activityAdult human liverAbundant cytochrome P450CYP3A4 promoterHuman liverPostnatal liverProtein 4DBP expressionPAR familyEmbryonic factorsHepG2 cellsTranscription factorsCytochrome P450LiverFunctional significanceCo-transfection experimentsBinding proteinPromoter activityExpressionReporter constructsFactorsFactor family
2006
Identification and functional analysis of Human FMO3 Genetic Variants
Hines R, Koukouritaki S, Poch M, Henderson M, Siddens L, Krueger S, VanDyke J, Romero A, Williams D. Identification and functional analysis of Human FMO3 Genetic Variants. The FASEB Journal 2006, 20: a264-a264. DOI: 10.1096/fasebj.20.4.a264-a.Peer-Reviewed Original ResearchPromoter activityFunctional analysisTransient expression analysisHaplotype AComplete lossHigher promoter activityNear complete lossTranscript splicingExpression analysisSynonymous SNPsReporter constructsIntron SNPSNP frequenciesStructural variantsObserved haplotypesMultiple substratesHaplotype DGenetic variantsExpression levelsSNPsEnzyme activityDNA samplesHaplotypesPromoter variantsVivo impact