2000
Diflubenzuron, a Benzoyl-Urea Insecticide, Is a Potent Inhibitor of TCDD-Induced CYP1A1 Expression in HepG2 Cells
Ledirac N, Delescluse C, Lesca P, Piechocki M, Hines R, de Sousa G, Pralavorio M, Rahmani R. Diflubenzuron, a Benzoyl-Urea Insecticide, Is a Potent Inhibitor of TCDD-Induced CYP1A1 Expression in HepG2 Cells. Toxicology And Applied Pharmacology 2000, 164: 273-279. PMID: 10799337, DOI: 10.1006/taap.2000.8920.Peer-Reviewed Original ResearchConceptsCYP1A1 expressionEthoxyresorufin-O-deethylase (EROD) activityHepG2 cellsStrong dose-dependent decreaseDose-dependent decreaseB16 murine melanomaHuman hepatoma cells HepG2Receptor antagonismTreatment of HepG2Hepatoma cells HepG2Human CYP1A1 promoterParental HepG2 cellsCYP1A1 inducerNoncytotoxic concentrationsMurine melanomaChloramphenicol acetyltransferase (CAT) reporter geneAdditional studiesCYP1A1 inductionNorthern blot analysisCell linesBlot analysisSynthesis inhibitorH exposurePotent inhibitorAh receptor
1993
Expression of human cytochrome P450 1A1 in DNA repair deficient and proficient human fibroblasts stably transformed with an inducible expression vector
States J, Quan T, Hines R, Novak R, Runge-Morris M. Expression of human cytochrome P450 1A1 in DNA repair deficient and proficient human fibroblasts stably transformed with an inducible expression vector. Carcinogenesis 1993, 14: 1643-1649. PMID: 8353849, DOI: 10.1093/carcin/14.8.1643.Peer-Reviewed Original ResearchMeSH Keywords7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxideAnimalsBase SequenceBenzo(a)pyreneBenzoflavonesBiotransformationCadmiumCadmium CompoundsChimeraCytochrome P-450 Enzyme SystemDNA RepairEnzyme InductionFibroblastsGene Expression Regulation, EnzymologicGenetic VectorsHumansIsoenzymesMiceMolecular Sequence DataPlasmidsRNA, MessengerSulfatesTransfectionTransformation, GeneticXeroderma PigmentosumConceptsCytochrome P450 1A1P450 1A1Inducible CYP1A1Inducible mouse metallothionein-I promoterLow dosesHuman skin fibroblastsProficient cellsPotent mutagenic agentCYP1A1 expressionCYP1A1 activityHuman cytochrome P450 1A1DNA repairCYP1A1Mouse metallothionein-I promoterCytotoxic effectsMetallothionein-I promoterSkin fibroblastsDifferential cytotoxicityDNA repair-proficient cellsToxic effectsMore cytotoxicCytochrome P450Normal cellsRepairHuman CYP1A1