Enzyme-Mediated Protein Haptenation of Dapsone and Sulfamethoxazole in Human Keratinocytes: I. Expression and Role of Cytochromes P450
Vyas P, Roychowdhury S, Khan F, Prisinzano T, Lamba J, Schuetz E, Blaisdell J, Goldstein J, Munson K, Hines R, Svensson C. Enzyme-Mediated Protein Haptenation of Dapsone and Sulfamethoxazole in Human Keratinocytes: I. Expression and Role of Cytochromes P450. Journal Of Pharmacology And Experimental Therapeutics 2006, 319: 488-496. PMID: 16857728, DOI: 10.1124/jpet.106.105858.Peer-Reviewed Original ResearchConceptsCutaneous drug reactionsNormal human epidermal keratinocytesEnzyme-linked immunosorbent assayDrug reactionsProtein haptenationCommon adverse drug reactionsLife-threatening complicationsAdverse drug reactionsCytochrome P450Presence of CYP3A4Immunofluorescence confocal microscopyConfocal microscopyImmune responseHuman epidermal keratinocytesNumerous minorArylamine drugsImmunosorbent assayCellular proteinsCYP2C9 proteinEpidermal keratinocytesHaptenationHuman keratinocytesChemical inhibitorsProtein adductionCyclooxygenase