2010
Allosteric inhibition of macrophage migration inhibitory factor revealed by ibudilast
Cho Y, Crichlow GV, Vermeire JJ, Leng L, Du X, Hodsdon ME, Bucala R, Cappello M, Gross M, Gaeta F, Johnson K, Lolis EJ. Allosteric inhibition of macrophage migration inhibitory factor revealed by ibudilast. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 11313-11318. PMID: 20534506, PMCID: PMC2895110, DOI: 10.1073/pnas.1002716107.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorMigration inhibitory factorInhibitory factorPeripheral blood mononuclear cellsBlood mononuclear cellsNeuropathic painOpioid withdrawalMIF receptorBronchial asthmaOcular indicationsMononuclear cellsProinflammatory proteinsAntiinflammatory drugsGlial cellsPossible therapeuticsAntiinflammatory activityChemotactic functionNonselective inhibitorAV411Major receptorTautomerase activityReceptorsAllosteric binding siteAntibody experimentsTreatment
2005
Discovery and pre-clinical development of antithrombotics from hematophagous invertebrates.
Ledizet M, Harrison LM, Koskia RA, Cappello M. Discovery and pre-clinical development of antithrombotics from hematophagous invertebrates. Cardiovascular & Hematological Agents In Medicinal Chemistry 2005, 3: 1-10. PMID: 15638739, DOI: 10.2174/1568016052773315.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsNumerous invertebrate speciesHematophagous invertebratesInvertebrate speciesSpecific inhibitorUnique specificityInvertebratesFunctional strategiesCritical roleImpressive arrayActivation of thrombosisUseful therapeutic agentPre-clinical developmentNatural productsInhibitorsPlatelet inhibitorsHeart diseaseHuman illnessPlatelet functionClinical developmentSpeciesTherapeutic agentsAntithromboticsParasitesBloodmealThrombosis
2003
Isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult Ancylostoma caninum
Del Valle A, Jones BF, Harrison LM, Chadderdon RC, Cappello M. Isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult Ancylostoma caninum. Molecular And Biochemical Parasitology 2003, 129: 167-177. PMID: 12850261, DOI: 10.1016/s0166-6851(03)00121-x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAnimalsAntibodies, HelminthChromatography, High Pressure LiquidCloning, MolecularCollagenDNA, ComplementaryFibrinogenHelminth ProteinsImmunoglobulin GImmunohistochemistryIntegrin alpha2beta1Molecular Sequence DataPlatelet Aggregation InhibitorsPlatelet Glycoprotein GPIIb-IIIa ComplexRecombinant ProteinsSpectrometry, Mass, Electrospray IonizationConceptsIron deficiency anemiaCell surface integrin receptorsIdentical N-terminalAmino acid sequence homologyAmino acid sequenceSingle gene productSurface integrin receptorsDeficiency anemiaMammalian hemostasisCorresponding native proteinsCDNA correspondingSignificant homologyMolecular cloningSimilar molecular massSecreted proteinsGene productsSequence homologyAcid sequenceInhibitor proteinHPI proteinVaccine-based strategiesNative proteinN-terminalAdult Ancylostoma caninumAdult stage
1999
The Hookworm Platelet Inhibitor: Functional Blockade of Integrins GPIIb/IIIa (αIIbβ3) and GPIa/IIa (α2β1) Inhibits Platelet Aggregation and Adhesion In Vitro
Chadderdon R, Cappello M. The Hookworm Platelet Inhibitor: Functional Blockade of Integrins GPIIb/IIIa (αIIbβ3) and GPIa/IIa (α2β1) Inhibits Platelet Aggregation and Adhesion In Vitro. The Journal Of Infectious Diseases 1999, 179: 1235-1241. PMID: 10191228, DOI: 10.1086/314724.Peer-Reviewed Original ResearchMeSH KeywordsAncylostomaAnimalsBlood PlateletsHumansIntegrinsPlatelet AdhesivenessPlatelet AggregationPlatelet Aggregation InhibitorsPlatelet Glycoprotein GPIIb-IIIa ComplexReceptors, CollagenConceptsIntegrin GPIIb/IIIaPlatelet inhibitorsSoluble protein extractsGPIIb/IIIaPlatelet aggregationMolecular massProtein extractsIron deficiency anemiaGPIa/IIaInhibitory monoclonal antibodiesVariety of agonistsAnti-platelet activityAdult Ancylostoma caninum hookwormsInhibits platelet aggregationPlatelet receptorsDeficiency anemiaFunctional blockadeSpecific blockadeBiologic roleSize exclusion chromatographyIntestinal nematodesSecretory productsMonoclonal antibodiesInhibitorsAdult worms