2006
Molecular cloning and characterization of a C-type lectin from Ancylostoma ceylanicum: Evidence for a role in hookworm reproductive physiology
Brown AC, Harrison LM, Kapulkin W, Jones BF, Sinha A, Savage A, Villalon N, Cappello M. Molecular cloning and characterization of a C-type lectin from Ancylostoma ceylanicum: Evidence for a role in hookworm reproductive physiology. Molecular And Biochemical Parasitology 2006, 151: 141-147. PMID: 17129620, PMCID: PMC1831819, DOI: 10.1016/j.molbiopara.2006.10.017.Peer-Reviewed Original ResearchConceptsC-type lectinRecombinant proteinsAmino acid mature proteinDrosophila S2 cellsPutative C-type lectinReproductive physiologySecretory signal sequenceEssential cell functionsSoluble protein extractsEgg cell membraneS2 cellsMature proteinCDNA correspondingProtein familyCDNA correspondsSignal sequenceMolecular cloningSperm proteinsRelated proteinsReverse transcription-PCRProtein extractsCell membraneProteinTranscription-PCRCell function
2003
Isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult Ancylostoma caninum
Del Valle A, Jones BF, Harrison LM, Chadderdon RC, Cappello M. Isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult Ancylostoma caninum. Molecular And Biochemical Parasitology 2003, 129: 167-177. PMID: 12850261, DOI: 10.1016/s0166-6851(03)00121-x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAnimalsAntibodies, HelminthChromatography, High Pressure LiquidCloning, MolecularCollagenDNA, ComplementaryFibrinogenHelminth ProteinsImmunoglobulin GImmunohistochemistryIntegrin alpha2beta1Molecular Sequence DataPlatelet Aggregation InhibitorsPlatelet Glycoprotein GPIIb-IIIa ComplexRecombinant ProteinsSpectrometry, Mass, Electrospray IonizationConceptsIron deficiency anemiaCell surface integrin receptorsIdentical N-terminalAmino acid sequence homologyAmino acid sequenceSingle gene productSurface integrin receptorsDeficiency anemiaMammalian hemostasisCorresponding native proteinsCDNA correspondingSignificant homologyMolecular cloningSimilar molecular massSecreted proteinsGene productsSequence homologyAcid sequenceInhibitor proteinHPI proteinVaccine-based strategiesNative proteinN-terminalAdult Ancylostoma caninumAdult stage
2000
A Broad Spectrum Kunitz Type Serine Protease Inhibitor Secreted by the Hookworm Ancylostoma ceylanicum *
Milstone A, Harrison L, Bungiro R, Kuzmic̆ P, Cappello M. A Broad Spectrum Kunitz Type Serine Protease Inhibitor Secreted by the Hookworm Ancylostoma ceylanicum *. Journal Of Biological Chemistry 2000, 275: 29391-29399. PMID: 10893410, DOI: 10.1074/jbc.m002715200.Peer-Reviewed Original ResearchConceptsKunitz-type serine protease inhibitorType serine protease inhibitorSerine protease inhibitorSecretory signal sequenceBlood-feeding hookwormsPET-28a plasmidSoluble protein extractsTight binding inhibitorMature proteinCDNA correspondingSignal sequenceParasitic nematodesInfective third-stage larvaeProtease inhibitorsRecombinant proteinsParasite survivalMolecular mechanismsMolecular massProtein extractsEscherichia coliExcretory/secretory productsSerine proteasesThird-stage larvaeAdult A. ceylanicumIron deficiency anemia