2022
Comparative Genomics of Disease and Carriage Serotype 1 Pneumococci
Chaguza C, Ebruke C, Senghore M, Lo S, Tientcheu P, Gladstone R, Tonkin-Hill G, Cornick J, Yang M, Worwui A, McGee L, Breiman R, Klugman K, Kadioglu A, Everett D, Mackenzie G, Croucher N, Roca A, Kwambana-Adams B, Antonio M, Bentley S. Comparative Genomics of Disease and Carriage Serotype 1 Pneumococci. Genome Biology And Evolution 2022, 14: evac052. PMID: 35439297, PMCID: PMC9048925, DOI: 10.1093/gbe/evac052.Peer-Reviewed Original ResearchConceptsGenomic variationGenome-wide association studiesComparative genomicsGenetic variationLineage distributionAssociation studiesPhenotypic variabilityPathogen surveillanceSystemic tissuesIsolatesSerotype 1GenomicsLineagesGeneticsMinimal effectVariationInvasivenessSerotype 1 pneumococciIsolationInvasive disease
2021
Serotype 1 pneumococcus: epidemiology, genomics, and disease mechanisms
Chaguza C, Yang M, Jacques L, Bentley S, Kadioglu A. Serotype 1 pneumococcus: epidemiology, genomics, and disease mechanisms. Trends In Microbiology 2021, 30: 581-592. PMID: 34949516, PMCID: PMC7613904, DOI: 10.1016/j.tim.2021.11.007.Peer-Reviewed Original ResearchComplete Genome Sequence of Streptococcus pneumoniae Strain BVJ1JL, a Serotype 1 Carriage Isolate from Malawi
Betts M, Jarvis S, Jeffries A, Gori A, Chaguza C, Msefula J, Weight CM, Kwambana-Adams B, French N, Swarthout TD, Brown JS, Heyderman RS. Complete Genome Sequence of Streptococcus pneumoniae Strain BVJ1JL, a Serotype 1 Carriage Isolate from Malawi. Microbiology Resource Announcements 2021, 10: e00715-21. PMID: 34591678, PMCID: PMC8483660, DOI: 10.1128/mra.00715-21.Peer-Reviewed Original Research
2020
Lower Density and Shorter Duration of Nasopharyngeal Carriage by Pneumococcal Serotype 1 (ST217) May Explain Its Increased Invasiveness over Other Serotypes
Bricio-Moreno L, Chaguza C, Yahya R, Shears RK, Cornick JE, Hokamp K, Yang M, Neill DR, French N, Hinton JCD, Everett DB, Kadioglu A. Lower Density and Shorter Duration of Nasopharyngeal Carriage by Pneumococcal Serotype 1 (ST217) May Explain Its Increased Invasiveness over Other Serotypes. MBio 2020, 11: e00814-20. PMID: 33293378, PMCID: PMC7733939, DOI: 10.1128/mbio.00814-20.Peer-Reviewed Original ResearchConceptsInvasive pneumococcal diseaseNasopharyngeal carriageSerotype 1Invasive diseaseDisease progressionPneumococcal strainsInvasive pneumococcal disease casesQueen Elizabeth Central HospitalMajor causePneumococcal disease casesPneumococcal serotype 1Health care systemPneumococcal diseaseCentral HospitalVaccine efficacyInvasive infectionsInvasive pneumoniaCommon causeMurine modelMouse modelDisease casesCarrier stateHuman nasopharynxPneumoniaRNA sequencing analysisHypervirulent pneumococcal serotype 1 harbours two pneumolysin variants with differential haemolytic activity
Panagiotou S, Chaguza C, Yahya R, Audshasai T, Baltazar M, Ressel L, Khandaker S, Alsahag M, Mitchell TJ, Prudhomme M, Kadioglu A, Yang M. Hypervirulent pneumococcal serotype 1 harbours two pneumolysin variants with differential haemolytic activity. Scientific Reports 2020, 10: 17313. PMID: 33057054, PMCID: PMC7560715, DOI: 10.1038/s41598-020-73454-w.Peer-Reviewed Original ResearchConceptsPneumococcal serotype 1Invasive pneumococcal diseaseSerotype 1Haemolytic activityVivo mouse modelDetectable haemolytic activityNeutrophil-mediated phagocytosisPneumococcal diseaseCommon causeInfection doseHuman reservoirMouse modelRapid onsetStreptococcus pneumoniaeDisease symptomsLethal doseClonal typesVirulence propertiesGlobal pathogenST306IgA proteaseSymptomsSequence typesMiceDoseBacterial genome-wide association study of hyper-virulent pneumococcal serotype 1 identifies genetic variation associated with neurotropism
Chaguza C, Yang M, Cornick JE, du Plessis M, Gladstone RA, Kwambana-Adams BA, Lo SW, Ebruke C, Tonkin-Hill G, Peno C, Senghore M, Obaro SK, Ousmane S, Pluschke G, Collard JM, Sigaùque B, French N, Klugman KP, Heyderman RS, McGee L, Antonio M, Breiman RF, von Gottberg A, Everett DB, Kadioglu A, Bentley SD. Bacterial genome-wide association study of hyper-virulent pneumococcal serotype 1 identifies genetic variation associated with neurotropism. Communications Biology 2020, 3: 559. PMID: 33033372, PMCID: PMC7545184, DOI: 10.1038/s42003-020-01290-9.Peer-Reviewed Original ResearchConceptsGenome-wide association studiesPneumococcal serotype 1Genetic variationBacterial genome-wide association studiesAssociation studiesSerotype 1Central nervous system infectionSignificant genotype-phenotype associationsNervous system infectionGenotype-phenotype associationsHelicase proteinPopulation structureCNS infectionsSystem infectionNegligible heritabilityMeningitis outbreakCNS tissueCerebrospinal fluidImmune evasionSerotype 1 strainPneumococcal survivalPolygenic etiologySmall effect sizesMeningitisTropism
2017
The global distribution and diversity of protein vaccine candidate antigens in the highly virulent Streptococcus pnuemoniae serotype 1
Cornick JE, Bishop Ö, Yalcin F, Kiran AM, Kumwenda B, Chaguza C, Govindpershad S, Ousmane S, Senghore M, du Plessis M, Pluschke G, Ebruke C, McGee L, Sigaùque B, Collard JM, Bentley SD, Kadioglu A, Antonio M, von Gottberg A, French N, Klugman KP, Heyderman RS, Alderson M, Everett DB, consortium F. The global distribution and diversity of protein vaccine candidate antigens in the highly virulent Streptococcus pnuemoniae serotype 1. Vaccine 2017, 35: 972-980. PMID: 28081968, PMCID: PMC5287219, DOI: 10.1016/j.vaccine.2016.12.037.Peer-Reviewed Original ResearchConceptsProtein vaccine candidatePneumococcal protein vaccinesProtein vaccineVaccine candidatesSerotype 1Multi-valent vaccinesSerotype 1 pneumococciPneumococcal conjugate vaccineHuman immune responseImportant pneumococcal serotypesConjugate vaccinePneumococcal diseasePneumococcal serotypesVaccine interventionsCommon causeImmune responseStudy populationAllelic variantsTarget antigenVaccineS. pneumoniae TIGR4Pneumococcal populationIntervention strategiesPCPADifferent allelic variants
2016
Understanding pneumococcal serotype 1 biology through population genomic analysis
Chaguza C, Cornick JE, Harris SR, Andam CP, Bricio-Moreno L, Yang M, Yalcin F, Ousmane S, Govindpersad S, Senghore M, Ebruke C, Du Plessis M, Kiran AM, Pluschke G, Sigauque B, McGee L, Klugman KP, Turner P, Corander J, Parkhill J, Collard JM, Antonio M, von Gottberg A, Heyderman RS, French N, Kadioglu A, Hanage WP, Everett DB, Bentley SD, for the PAGe Consortium. Understanding pneumococcal serotype 1 biology through population genomic analysis. BMC Infectious Diseases 2016, 16: 649. PMID: 27821148, PMCID: PMC5100261, DOI: 10.1186/s12879-016-1987-z.Peer-Reviewed Original ResearchConceptsDisease burdenPneumococcal disease burdenSerotype 1 pneumococciSub-Saharan AfricaHigh prevalenceHuman nasopharynxAfrican isolatesLow-income countriesSerotype 1Longer durationFurther studiesTetracycline resistanceBiological mechanismsST217West African isolatesDifferent geographical regionsSuccessful clones